Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant klebsiella pneumoniae

We characterized six drug-resistant nosocomial isolates of Klebsiella pneumoniae obtained in a hospital located in northern Minas Gerais State, Brazil, by determining their antibiotic sensitivity profiles, detecting the blaKPC genetic marker and examining their clonal relationships. All isolates wer...

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Detalles Bibliográficos
Autores: Emerson Ribeiro Lima, L. L. S. Cardoso, Alessandra Rejane Ericsson de Oliveira Xavier, Adriana Amaral Carvalho, Mauro Aparecido de Sousa Xavier, Hadison Santos Nogueira Curzio, Higor Rabelo Guedes, Luciano Freitas Fernandes, Anna Christina de Almeida, Elytania Veiga Menezes, Nair Amélia Prates Barreto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Institución:Universidade Federal de Minas Gerais (UFMG)
Repositorio:Repositório Institucional da UFMG
Idioma:inglés
OAI Identifier:oai:repositorio.ufmg.br:1843/42345
Acceso en línea:http://doi.org/10.4238/GMR18172
http://hdl.handle.net/1843/42345
https://orcid.org/ 0000-0001-9836-4117
Access Level:acceso abierto
Palabra clave:Bacterias gram-negativas
Infecções por Bactérias Gram-Negativas
Descripción
Sumario:We characterized six drug-resistant nosocomial isolates of Klebsiella pneumoniae obtained in a hospital located in northern Minas Gerais State, Brazil, by determining their antibiotic sensitivity profiles, detecting the blaKPC genetic marker and examining their clonal relationships. All isolates were found to be extensively drug resistant. A PCR assay was used to confirm the identity of the isolates as K. pneumoniae and assess the blaKPC gene. All isolates tested positive for the blaKPC gene, which is related to carbapenem resistance. The genetic profiles and clonal relationships among the isolates were evaluated by ERIC-PCR. All the isolates were in a single group with two distinct subgroups. Analysis of the genetic diversity among the isolates revealed that five of the six were clones, which suggests cross-transmission in this hospital environment. Five of the patients died from infection. We describe the first detection of KPC-producing K. pneumoniae isolates from a hospital in northern Minas Gerais state.

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