Biocomposite-based fibrous scaffolds of natural rubber/polyhydroxybutyrate blend reinforced with 45S5 bioglass aiming at biomedical applications

The solution blow spinning technique was used to fabricate a new biocomposite fibrous mat consisting of natural rubber (NR) and polyhydroxybutyrate (PHB) bioblend, with various loads of 45S5 bioglass (BG) particles. According to SEM analysis, NR fibers exhibited ribbon-like morphologies, whereas the...

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Detalles Bibliográficos
Autores: Silva, Michael J. [UNESP], Dias, Yasmin J., Zaszczyńska, Angelika, Robles, Jaqueline Rojas, Abiade, Jeremiah, Kowalczyk, Tomasz, Kołbuk, Dorota, Sajkiewicz, Paweł Ł., Yarin, Alexander L.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/306988
Acceso en línea:http://dx.doi.org/10.1002/pc.27839
https://hdl.handle.net/11449/306988
Access Level:acceso abierto
Palabra clave:45S5 bioglass
biocomposite fibrous mat
biomedical applications
natural rubber
polyhydroxybutyrate
solution blow spinning
Descripción
Sumario:The solution blow spinning technique was used to fabricate a new biocomposite fibrous mat consisting of natural rubber (NR) and polyhydroxybutyrate (PHB) bioblend, with various loads of 45S5 bioglass (BG) particles. According to SEM analysis, NR fibers exhibited ribbon-like morphologies, whereas the addition of PHB resulted in improved fiber formation and a reduction in their diameter. In NR-PHB/BG biocomposites with varying BG loadings, typical thermal degradation events of PHB (stage i) and NR (stage ii) were observed. In comparison with pure PHB, the TG/DTG curves of NR-PHB/BG specimens revealed a lower stage i degradation peak. Such an outcome is possibly due to the fact that PHB in the NR-PHB fibers is located predominantly at the surface, that is, PHB is more susceptible to thermal degradation. The NR-PHB/BG biocomposite possessed an increased stiffness due to the addition of PHB and BG, resulting in an increased stress and a decreased strain at rupture compared to the pure NR and NR-PHB mats. DMA analysis revealed two well-defined regions, above and below the glass transition temperature (Tg), for the storage modulus (E') of the NR-PHB/BG specimens. The values of E' were in both regions for NR-PHB/BG specimens increased at higher BG content. The measured tanδ = E″/E' was used to determine the Tg value for all specimens, with Tg found to be in the −49 to −46°C range. Finally, NR-PHB/BG biocomposite fibrous were proven noncytotoxic by in-vitro testing on fibroblasts. These biocomposites enhanced cell growth, holding great promise for tissue engineering applications. Highlights: Solution blow spinning technique was used to produce three-phase biocomposite specimens. NR-PHB/BG fibrous mat specimens with a diameter of 9–10 μm were obtained. Although high BG loads are applied to the NR-PHB/BG specimens, they remain elastic and flexible. Fibrous biocomposite mats enhance cell growth and possess great potential for tissue engineering.