Rt-qpcr-based pool testing for the diagnosis of covid-19

This study proposes a strategy for large-scale testing among a large number of people for the early diagnosis of COVID-19 to elucidate the epidemiological situation. Pool testing involves the analysis of pooled samples. This study aimed to discuss a reverse transcription technique followed by quanti...

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Bibliographic Details
Authors: Hugo Itarusato, Santuza Maria Ribeiro Teixeira, Murilo Soares Costa, Ricardo Hiroshi Caldeira Takahashi, Karine Lima Lourenço, Nathalia Sernizon Guimarães, Claudia Regina Lindgren Alves, Elaine Leandro Machado, Unaí Tupinambás, Flávio Guimarães da Fonseca
Format: article
Status:Published version
Publication Date:2023
Country:Brasil
Institution:Universidade Federal de Minas Gerais (UFMG)
Repository:Repositório Institucional da UFMG
Language:English
OAI Identifier:oai:repositorio.ufmg.br:1843/78639
Online Access:http://hdl.handle.net/1843/78639
Access Level:Open access
Keyword:COVID-19
Coronavirus Infections
SARS-CoV-2
Pandemics
Reverse Transcriptase Polymerase Chain Reaction
Description
Summary:This study proposes a strategy for large-scale testing among a large number of people for the early diagnosis of COVID-19 to elucidate the epidemiological situation. Pool testing involves the analysis of pooled samples. This study aimed to discuss a reverse transcription technique followed by quantitativereal-time polymerase chain reaction using pool testing to detect SARS-CoV-2 in nasopharyngeal swab samples. The study proposes an innovative diagnostic strategy that contributes to resource optimization, cost reduction, and improved agility of feedback from results.

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