Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil

Between September 11 and 20, 1996, an outbreak of bacteremia occurred in patients undergoing hemodialysis at one dialysis center in Campinas, São Paulo, Brazil. Water and dialysate samples as well as blood samples from patients with bacteremia were collected for bacteriological analysis. All Pseudom...

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Autores: Pisani, Beatriz, Simões, Marise, Prandi, Maria Angela G., Rocha, Marilu M. M., Gonçalves, Célia R., Vaz, Tânia M. I., Irino, Kinue
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Brasil
Recursos:Instituto Adolfo Lutz
Repositorio:Revista do Instituto Adolfo Lutz (Online)
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.saude.sp.gov.br:article/35054
Acesso em linha:https://periodicos.saude.sp.gov.br/RIAL/article/view/35054
Access Level:acceso abierto
Palavra-chave:Hemodialysis
bacteremia
Pseudomonas aeruginosa
serotyping
pyocintyping
ribotyping
Hemodiálise
água
bacteriemia
sorotipagem
piocinotipagem
ribotipagem
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spelling Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, BrazilSurto de bacteriemia por Pseudomonas aeruginosa na unidade de hemodiálise de um hospital de Campinas, São Paulo, BrasilHemodialysisbacteremiaPseudomonas aeruginosaserotypingpyocintypingribotypingHemodiáliseáguabacteriemiaPseudomonas aeruginosasorotipagempiocinotipagemribotipagemBetween September 11 and 20, 1996, an outbreak of bacteremia occurred in patients undergoing hemodialysis at one dialysis center in Campinas, São Paulo, Brazil. Water and dialysate samples as well as blood samples from patients with bacteremia were collected for bacteriological analysis. All Pseudomonasaeruginosa strains were serotyped and pyocin-typed. P. aeruginosa strains belonging to the same serotype (O15) and pyocin type (P10) were submitted to antimicrobial susceptibility testing and to ribotyping using two restriction enzymes, EcoRI and BamHI. The high concentrations of bacteria detected in all hemodialyzersand their heavy rate of contamination (86.7%) by Pseudomonas aeruginosa or Burkholderia cepacia showed that this dialysis center was operating in inadequate conditions. One strain of P. aeruginosa isolated from hemodialyzer and the strains recovered from blood cultures of patients showed similar phenotypical and genetic traits which suggest a common source of infection in this oubreak. We report the potential risk for patients undergoing hemodialysis to acquire infections due to the inadequate practice of reusing disposable dialyzers during the reprocessing procedures.Foram analisadas amostras de água e de dializados coletados de diferentes pontos do sistema de hemodiálise de um Centro de Hemodiálise de um hospital de Campinas, São Paulo, Brasil, após um surto de bacteriemia ocorrido em setembro de 1996. As amostras foram submetidas à contagem de bactérias heterotróficas e pesquisa de coliformes totais, assim como foram realizadas as hemoculturas dos pacientes com bacteriemia. As cepas isoladas e identificadas como Pseudomonas aeruginosa foram submetidas à sorotipagem e à piocinotipagem e, naquelas pertencentes ao mesmo sorotipo e piocinotipo, procurou-se determinar o perfil de sensibilidade aos agentes antimicrobianos e o ribotipo. Quanto à pesquisa de Pseudomonas, 80% das amostras  correspondentes à segunda coleta foram positivas e em todas as amostras referentes à terceira coleta foram isoladas Pseudomonas aeruginosa ou Bulkholderia cepacia. Apenas uma cepa de P.aeruginosa de água pertencia aos mesmos sorotipo (O15) e piocinotipo (P10) daqueles obtidos dos pacientes. A compatibilidade genética das amostras das duas origens, verificada pela ribotipagem, sugere um veículo comum na propagação da infecção.Instituto Adolfo Lutz2000-12-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3505410.53393/rial.2000.59.35054Instituto Adolfo Lutz Journal - RIAL; Vol. 59 No. 1-2 (2000); 51-6Revista del Instituto Adolfo Lutz - RIAL; Vol. 59 Núm. 1-2 (2000); 51-6Revista do Instituto Adolfo Lutz; v. 59 n. 1-2 (2000); 51-61983-38140073-985510.18241/rial.v59i1/2reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/35054/33532https://periodicos.saude.sp.gov.br/RIAL/article/view/35054/36807Copyright (c) 2000 Beatriz Pisani, Marise Simões, Maria Angela G. Prandi, Marilu M. M. Rocha, Célia R. Gonçalves, Tânia M. I. Vaz, Kinue Irinoinfo:eu-repo/semantics/openAccessPisani, Beatriz Simões, Marise Prandi, Maria Angela G. Rocha, Marilu M. M. Gonçalves, Célia R. Vaz, Tânia M. I. Irino, Kinue 2024-01-24T17:57:26Zoai:ojs.periodicos.saude.sp.gov.br:article/35054Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2024-01-24T17:57:26Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
Surto de bacteriemia por Pseudomonas aeruginosa na unidade de hemodiálise de um hospital de Campinas, São Paulo, Brasil
title Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
spellingShingle Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
Pisani, Beatriz
Hemodialysis
bacteremia
Pseudomonas aeruginosa
serotyping
pyocintyping
ribotyping
Hemodiálise
água
bacteriemia
Pseudomonas aeruginosa
sorotipagem
piocinotipagem
ribotipagem
title_short Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
title_full Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
title_fullStr Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
title_full_unstemmed Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
title_sort Outbreak of Pseudomonas aeruginosa bacteremia in a Hemodialysis Center in Campinas, São Paulo, Brazil
dc.creator.none.fl_str_mv Pisani, Beatriz
Simões, Marise
Prandi, Maria Angela G.
Rocha, Marilu M. M.
Gonçalves, Célia R.
Vaz, Tânia M. I.
Irino, Kinue
author Pisani, Beatriz
author_facet Pisani, Beatriz
Simões, Marise
Prandi, Maria Angela G.
Rocha, Marilu M. M.
Gonçalves, Célia R.
Vaz, Tânia M. I.
Irino, Kinue
author_role author
author2 Simões, Marise
Prandi, Maria Angela G.
Rocha, Marilu M. M.
Gonçalves, Célia R.
Vaz, Tânia M. I.
Irino, Kinue
author2_role author
author
author
author
author
author
dc.subject.por.fl_str_mv Hemodialysis
bacteremia
Pseudomonas aeruginosa
serotyping
pyocintyping
ribotyping
Hemodiálise
água
bacteriemia
Pseudomonas aeruginosa
sorotipagem
piocinotipagem
ribotipagem
topic Hemodialysis
bacteremia
Pseudomonas aeruginosa
serotyping
pyocintyping
ribotyping
Hemodiálise
água
bacteriemia
Pseudomonas aeruginosa
sorotipagem
piocinotipagem
ribotipagem
description Between September 11 and 20, 1996, an outbreak of bacteremia occurred in patients undergoing hemodialysis at one dialysis center in Campinas, São Paulo, Brazil. Water and dialysate samples as well as blood samples from patients with bacteremia were collected for bacteriological analysis. All Pseudomonasaeruginosa strains were serotyped and pyocin-typed. P. aeruginosa strains belonging to the same serotype (O15) and pyocin type (P10) were submitted to antimicrobial susceptibility testing and to ribotyping using two restriction enzymes, EcoRI and BamHI. The high concentrations of bacteria detected in all hemodialyzersand their heavy rate of contamination (86.7%) by Pseudomonas aeruginosa or Burkholderia cepacia showed that this dialysis center was operating in inadequate conditions. One strain of P. aeruginosa isolated from hemodialyzer and the strains recovered from blood cultures of patients showed similar phenotypical and genetic traits which suggest a common source of infection in this oubreak. We report the potential risk for patients undergoing hemodialysis to acquire infections due to the inadequate practice of reusing disposable dialyzers during the reprocessing procedures.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/35054
10.53393/rial.2000.59.35054
url https://periodicos.saude.sp.gov.br/RIAL/article/view/35054
identifier_str_mv 10.53393/rial.2000.59.35054
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/35054/33532
https://periodicos.saude.sp.gov.br/RIAL/article/view/35054/36807
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 59 No. 1-2 (2000); 51-6
Revista del Instituto Adolfo Lutz - RIAL; Vol. 59 Núm. 1-2 (2000); 51-6
Revista do Instituto Adolfo Lutz; v. 59 n. 1-2 (2000); 51-6
1983-3814
0073-9855
10.18241/rial.v59i1/2
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
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