Avaliação da atividade antiviral do BromAc® para o tratamento da COVID-19
COVID-19 is one of the most significant challenges facing modern civilization and has not yet been eradicated. SARS-CoV-2 is a virus that can mutate and evade the antibodies generated by viral exposure or vaccination and, as a result, SARS-CoV-2 remains an imminent threat to public health. Currently...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | Brasil |
| Institución: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/65077 |
| Acceso en línea: | http://hdl.handle.net/1843/65077 |
| Access Level: | acceso abierto |
| Palabra clave: | COVID-19 N-acetilcisteína Bromelina BromAc® SARS-CoV-2 Microbiologia Covid-19 Betacoronavirus Acetilcisteína Bromelaínas |
| Sumario: | COVID-19 is one of the most significant challenges facing modern civilization and has not yet been eradicated. SARS-CoV-2 is a virus that can mutate and evade the antibodies generated by viral exposure or vaccination and, as a result, SARS-CoV-2 remains an imminent threat to public health. Currently, there is no 100% effective antiviral treatment for the severe form of COVID-19. Although treatment with dexamethasone and mechanical ventilation is the standard procedure for treating the disease, some patients still succumb to the infection. In this regard, BromAc® is a combination of bromelain and N-acetylcysteine, which has shown mucolytic effect and robust anti-inflammatory properties. Therefore, in the present study, we performed in vitro and ex vivo analyses to evaluate the effect of bromelain in combination with N-acetylcysteine on virus containment at different levels. The results indicated virucidal and dose-dependent activity of the combined compounds in vitro on the Vero-ACE2/TMPRSS2 cell line infected with the ômicron variant. In addition, the combined compounds reduced the number of genomic copies of SARS-CoV-2 RNA in tracheal aspirate samples from severely ill COVID-19 patients. BromAc® also promoted the cleavage of the S1 subunit of the Spike protein in tracheal aspirate samples, which brings new evidence of antiviral activity in samples from COVID-19 patients. In summary, these results bring to light evidence of ex vivo antiviral activity of the combination of bromelain and N-acetylcysteine that suggests its potential as a nebulization approach for treating COVID-19. |
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