Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions

Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression...

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Autores: Costa, Maurício B., Mimura, Kallyne K.O. [UNESP], Freitas, Aline A., Hungria, Emerith M., Sousa, Ana Lúcia O.M., Oliani, Sonia M. [UNESP], Stefani, Mariane M.A.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/176099
Acesso em linha:http://dx.doi.org/10.1016/j.micpath.2018.03.050
http://hdl.handle.net/11449/176099
Access Level:acceso abierto
Palavra-chave:Annexin A1
Chymase
Leprosy
Mast cell
Skin diseases
Tryptase
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spelling Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesionsAnnexin A1ChymaseLeprosyMast cellSkin diseasesTryptaseMast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try+ and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm2 were evaluated. Try+/chy+ MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try+ MCs outnumbered chy+ in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try+/chy+ subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Tropical Pathology and Public Health Institute Federal University of GoiásDepartment of Biology Instituto de Biociências Letras e Ciências Exatas Sao Paulo State University UNESPDepartment of Biology Instituto de Biociências Letras e Ciências Exatas Sao Paulo State University UNESPCAPES: #1054292Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)2018-12-11T17:19:03Z2018-12-11T17:19:03Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article277-284application/pdfhttp://dx.doi.org/10.1016/j.micpath.2018.03.050Microbial Pathogenesis, v. 118, p. 277-284.1096-12080882-4010http://hdl.handle.net/11449/17609910.1016/j.micpath.2018.03.0502-s2.0-850445899522-s2.0-85044589952.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,7510,751info:eu-repo/semantics/openAccessCosta, Maurício B.Mimura, Kallyne K.O. [UNESP]Freitas, Aline A.Hungria, Emerith M.Sousa, Ana Lúcia O.M.Oliani, Sonia M. [UNESP]Stefani, Mariane M.A.2024-10-25T14:11:19Zoai:repositorio.unesp.br:11449/176099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-25T14:11:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
spellingShingle Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
Costa, Maurício B.
Annexin A1
Chymase
Leprosy
Mast cell
Skin diseases
Tryptase
title_short Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_full Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_fullStr Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_full_unstemmed Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_sort Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
dc.creator.none.fl_str_mv Costa, Maurício B.
Mimura, Kallyne K.O. [UNESP]
Freitas, Aline A.
Hungria, Emerith M.
Sousa, Ana Lúcia O.M.
Oliani, Sonia M. [UNESP]
Stefani, Mariane M.A.
author Costa, Maurício B.
author_facet Costa, Maurício B.
Mimura, Kallyne K.O. [UNESP]
Freitas, Aline A.
Hungria, Emerith M.
Sousa, Ana Lúcia O.M.
Oliani, Sonia M. [UNESP]
Stefani, Mariane M.A.
author_role author
author2 Mimura, Kallyne K.O. [UNESP]
Freitas, Aline A.
Hungria, Emerith M.
Sousa, Ana Lúcia O.M.
Oliani, Sonia M. [UNESP]
Stefani, Mariane M.A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv Annexin A1
Chymase
Leprosy
Mast cell
Skin diseases
Tryptase
topic Annexin A1
Chymase
Leprosy
Mast cell
Skin diseases
Tryptase
description Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try+ and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm2 were evaluated. Try+/chy+ MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try+ MCs outnumbered chy+ in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try+/chy+ subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:19:03Z
2018-12-11T17:19:03Z
2018-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.micpath.2018.03.050
Microbial Pathogenesis, v. 118, p. 277-284.
1096-1208
0882-4010
http://hdl.handle.net/11449/176099
10.1016/j.micpath.2018.03.050
2-s2.0-85044589952
2-s2.0-85044589952.pdf
url http://dx.doi.org/10.1016/j.micpath.2018.03.050
http://hdl.handle.net/11449/176099
identifier_str_mv Microbial Pathogenesis, v. 118, p. 277-284.
1096-1208
0882-4010
10.1016/j.micpath.2018.03.050
2-s2.0-85044589952
2-s2.0-85044589952.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbial Pathogenesis
0,751
0,751
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 277-284
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15.198674

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