Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil

Hegger Fritsch; Jaqueline Lima; Lenisa Santos; Luciana Silva; Vanessa Nardy; Marcela Kelly Gómez Astete; Beatriz Senra Álvares da Silva Santos; Nágila Rocha Aguilar; Maria Isabel Maldonado Coelho Guedes; Guilherme Canhestro de Faria; Ronaldo Furtini; Felicidade Mota Pereira; Safira Rachel Milanez Drumond; Gabriel Muricy Cunha; Marcia São Pedro Leal Souza; Ronaldo de Jesus; Sara A. Franco Guimarães; Italo Coelho Nuno; Ian Carlos Brito de Santana; José Eduardo Ungar de sá; George Roma Santos; Willadesmon Santos Silva; Erica Azevedo Costa; Thiago Ferreira Guedes; Emerson Luiz Lima Araújo; Rodrigo Fabiano do Carmo Said; Carlos Frederico Campelo de Albuquerque; Cassio Roberto Leonel Peterka; Alessandro Pecego Martins Romano; Rivaldo Venâncio da Cunha; Ana Maria Bispo de Filippis; Arabela Leal e Silva de Mello; Marta Giovanetti; Vagner Fonseca; Luiz Carlos Junior Alcantara; Stephane Tosta; Joilson Xavier; Flavia Levy; Carla de Oliveira; Gabriela Menezes

Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil

settingsOrder Article Reprints Open AccessBrief Report Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil by Hegger Fritsch 1,†,Felicidade Mota Pereira 2,†,Erica Azevedo Costa 1,†ORCID,Vagner Fonseca 3,†ORCID,Stephane Tosta 1,†,Joilson...

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Autores:	Hegger Fritsch, Jaqueline Lima, Lenisa Santos, Luciana Silva, Vanessa Nardy, Marcela Kelly Gómez Astete, Beatriz Senra Álvares da Silva Santos, Nágila Rocha Aguilar, Maria Isabel Maldonado Coelho Guedes, Guilherme Canhestro de Faria, Ronaldo Furtini, Felicidade Mota Pereira, Safira Rachel Milanez Drumond, Gabriel Muricy Cunha, Marcia São Pedro Leal Souza, Ronaldo de Jesus, Sara A. Franco Guimarães, Italo Coelho Nuno, Ian Carlos Brito de Santana, José Eduardo Ungar de sá, George Roma Santos, Willadesmon Santos Silva, Erica Azevedo Costa, Thiago Ferreira Guedes, Emerson Luiz Lima Araújo, Rodrigo Fabiano do Carmo Said, Carlos Frederico Campelo de Albuquerque, Cassio Roberto Leonel Peterka, Alessandro Pecego Martins Romano, Rivaldo Venâncio da Cunha, Ana Maria Bispo de Filippis, Arabela Leal e Silva de Mello, Marta Giovanetti, Vagner Fonseca, Luiz Carlos Junior Alcantara, Stephane Tosta, Joilson Xavier, Flavia Levy, Carla de Oliveira, Gabriela Menezes
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2022
País:	Brasil
Institución:	Universidade Federal de Minas Gerais (UFMG)
Repositorio:	Repositório Institucional da UFMG
Idioma:	inglés
OAI Identifier:	oai:repositorio.ufmg.br:1843/67591
Acceso en línea:	https://doi.org/10.3390/v14071540 http://hdl.handle.net/1843/67591 https://orcid.org/0000-0002-5396-3818 https://orcid.org/0000-0001-5521-6448 https://orcid.org/0000-0003-4426-6989 https://orcid.org/0000-0003-3916-2018 https://orcid.org/0000-0002-1497-4397 https://orcid.org/0000-0002-6734-0216 https://orcid.org/0000-0002-7684-2883 https://orcid.org/0000-0002-5849-7326 https://orcid.org/0000-0002-6769-9931
Access Level:	acceso abierto
Palabra clave:	WNV Nanopore sequencing Genomic monitoring Brazil Genoma Brasil Equino Vírus do Nilo Ocidental

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dc.title.none.fl_str_mv	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
title	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
spellingShingle	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil Hegger Fritsch WNV Nanopore sequencing Genomic monitoring Brazil Genoma Brasil Equino Vírus do Nilo Ocidental
title_short	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
title_full	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
title_fullStr	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
title_full_unstemmed	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
title_sort	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil
dc.creator.none.fl_str_mv	Hegger Fritsch Jaqueline Lima Lenisa Santos Luciana Silva Vanessa Nardy Marcela Kelly Gómez Astete Beatriz Senra Álvares da Silva Santos Nágila Rocha Aguilar Maria Isabel Maldonado Coelho Guedes Guilherme Canhestro de Faria Ronaldo Furtini Felicidade Mota Pereira Safira Rachel Milanez Drumond Gabriel Muricy Cunha Marcia São Pedro Leal Souza Ronaldo de Jesus Sara A. Franco Guimarães Italo Coelho Nuno Ian Carlos Brito de Santana José Eduardo Ungar de sá George Roma Santos Willadesmon Santos Silva Erica Azevedo Costa Thiago Ferreira Guedes Emerson Luiz Lima Araújo Rodrigo Fabiano do Carmo Said Carlos Frederico Campelo de Albuquerque Cassio Roberto Leonel Peterka Alessandro Pecego Martins Romano Rivaldo Venâncio da Cunha Ana Maria Bispo de Filippis Arabela Leal e Silva de Mello Marta Giovanetti Vagner Fonseca Luiz Carlos Junior Alcantara Stephane Tosta Joilson Xavier Flavia Levy Carla de Oliveira Gabriela Menezes
author	Hegger Fritsch
author_facet	Hegger Fritsch Jaqueline Lima Lenisa Santos Luciana Silva Vanessa Nardy Marcela Kelly Gómez Astete Beatriz Senra Álvares da Silva Santos Nágila Rocha Aguilar Maria Isabel Maldonado Coelho Guedes Guilherme Canhestro de Faria Ronaldo Furtini Felicidade Mota Pereira Safira Rachel Milanez Drumond Gabriel Muricy Cunha Marcia São Pedro Leal Souza Ronaldo de Jesus Sara A. Franco Guimarães Italo Coelho Nuno Ian Carlos Brito de Santana José Eduardo Ungar de sá George Roma Santos Willadesmon Santos Silva Erica Azevedo Costa Thiago Ferreira Guedes Emerson Luiz Lima Araújo Rodrigo Fabiano do Carmo Said Carlos Frederico Campelo de Albuquerque Cassio Roberto Leonel Peterka Alessandro Pecego Martins Romano Rivaldo Venâncio da Cunha Ana Maria Bispo de Filippis Arabela Leal e Silva de Mello Marta Giovanetti Vagner Fonseca Luiz Carlos Junior Alcantara Stephane Tosta Joilson Xavier Flavia Levy Carla de Oliveira Gabriela Menezes
author_role	author
author2	Jaqueline Lima Lenisa Santos Luciana Silva Vanessa Nardy Marcela Kelly Gómez Astete Beatriz Senra Álvares da Silva Santos Nágila Rocha Aguilar Maria Isabel Maldonado Coelho Guedes Guilherme Canhestro de Faria Ronaldo Furtini Felicidade Mota Pereira Safira Rachel Milanez Drumond Gabriel Muricy Cunha Marcia São Pedro Leal Souza Ronaldo de Jesus Sara A. Franco Guimarães Italo Coelho Nuno Ian Carlos Brito de Santana José Eduardo Ungar de sá George Roma Santos Willadesmon Santos Silva Erica Azevedo Costa Thiago Ferreira Guedes Emerson Luiz Lima Araújo Rodrigo Fabiano do Carmo Said Carlos Frederico Campelo de Albuquerque Cassio Roberto Leonel Peterka Alessandro Pecego Martins Romano Rivaldo Venâncio da Cunha Ana Maria Bispo de Filippis Arabela Leal e Silva de Mello Marta Giovanetti Vagner Fonseca Luiz Carlos Junior Alcantara Stephane Tosta Joilson Xavier Flavia Levy Carla de Oliveira Gabriela Menezes
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.por.fl_str_mv	WNV Nanopore sequencing Genomic monitoring Brazil Genoma Brasil Equino Vírus do Nilo Ocidental
topic	WNV Nanopore sequencing Genomic monitoring Brazil Genoma Brasil Equino Vírus do Nilo Ocidental
description	settingsOrder Article Reprints Open AccessBrief Report Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil by Hegger Fritsch 1,†,Felicidade Mota Pereira 2,†,Erica Azevedo Costa 1,†ORCID,Vagner Fonseca 3,†ORCID,Stephane Tosta 1,†,Joilson Xavier 1ORCID,Flavia Levy 4,Carla de Oliveira 4,Gabriela Menezes 2,Jaqueline Lima 2,Lenisa Santos 2,Luciana Silva 2,Vanessa Nardy 2,Marcela Kelly Gómez Astete 2ORCID,Beatriz Senra Álvares da Silva Santos 1ORCID,Nágila Rocha Aguiar 1,Maria Isabel Maldonado Coelho Guedes 1ORCID,Guilherme Canhestro de Faria 5,Ronaldo Furtini 5,Safira Rachel Milanez Drumond 5,add Show full author list 1 Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil 2 Laboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, Brazil 3 Organização Pan-Americana de Saúde/Organização Mundial de Saúde, Brasilia 37650-000, Brazil 4 Laboratorio de Flavivirus, lnstituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil 5 Laboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, Brazil 6 Secretary of Health of the State of Bahia (SESAB), Salvador 40301-110, Brazil 7 Coordenação Geral dos Laboratórios de Saúde Pública, Secretaria de Vigilância em Saúde-Brazil, Brasília 70719-040, Brazil 8 Coordenação Geral das Arboviroses, Secretaria de Vigilância em Saúde (CGARB/SVS-MS), Brasilia 37650-000, Brazil 9 Fundação Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro 21040-360, Brazil 10 Department of Science and Technology for Humans and the Environment, University of Campus Bio-Medico, 00128 Rome, Italy * Authors to whom correspondence should be addressed. † These authors contributed equally to this work. Viruses 2022, 14(7), 1540; https://doi.org/10.3390/v14071540 Submission received: 17 June 2022 / Revised: 10 July 2022 / Accepted: 11 July 2022 / Published: 14 July 2022 (This article belongs to the Special Issue Usutu, West Nile and Tickborne Encephalitis Viruses) Downloadkeyboard_arrow_down Browse Figure Versions Notes Abstract During these past years, several studies have provided serological evidence regarding the circulation of West Nile virus (WNV) in Brazil. Despite some reports, much is still unknown regarding the genomic diversity and transmission dynamics of this virus in the country. Recently, genomic monitoring activities in horses revealed the circulation of WNV in several Brazilian regions. These findings on the paucity of genomic data reinforce the need for prompt investigation of WNV infection in horses, which may precede human cases of encephalitis in Brazil. Thus, in this study, we retrospectively screened 54 suspicious WNV samples collected between 2017 and 2020 from the spinal cord and brain of horses with encephalitis and generated three new WNV genomes from the Ceará and Bahia states, located in the northeastern region of Brazil. The Bayesian reconstruction revealed that at least two independent introduction events occurred in Brazil. The first introduction event appears to be likely related to the North American outbreak, and was estimated to have occurred in March 2013.The second introduction event appears to have occurred in September 2017 and appears to be likely related to the South American outbreak. Together, our results reinforce the importance of increasing the priority of WNV genomic monitoring in equines with encephalitis in order to track the dispersion of this emerging pathogen through the country.
publishDate	2022
dc.date.none.fl_str_mv	2022-07-14 2024-04-23T14:42:46Z 2024-04-23T14:42:46Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	https://doi.org/10.3390/v14071540 1999-4915 http://hdl.handle.net/1843/67591 https://orcid.org/0000-0002-5396-3818 https://orcid.org/0000-0001-5521-6448 https://orcid.org/0000-0003-4426-6989 https://orcid.org/0000-0003-3916-2018 https://orcid.org/0000-0002-1497-4397 https://orcid.org/0000-0002-6734-0216 https://orcid.org/0000-0002-7684-2883 https://orcid.org/0000-0002-5849-7326 https://orcid.org/0000-0002-6769-9931
url	https://doi.org/10.3390/v14071540 http://hdl.handle.net/1843/67591 https://orcid.org/0000-0002-5396-3818 https://orcid.org/0000-0001-5521-6448 https://orcid.org/0000-0003-4426-6989 https://orcid.org/0000-0003-3916-2018 https://orcid.org/0000-0002-1497-4397 https://orcid.org/0000-0002-6734-0216 https://orcid.org/0000-0002-7684-2883 https://orcid.org/0000-0002-5849-7326 https://orcid.org/0000-0002-6769-9931
identifier_str_mv	1999-4915
dc.language.iso.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	Viruses
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	pdf application/pdf
dc.publisher.none.fl_str_mv	Universidade Federal de Minas Gerais Brasil VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA VETER - ESCOLA DE VETERINARIA UFMG
publisher.none.fl_str_mv	Universidade Federal de Minas Gerais Brasil VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA VETER - ESCOLA DE VETERINARIA UFMG
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repository.mail.fl_str_mv	repositorio@ufmg.br
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spelling	Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in BrazilWNVNanopore sequencingGenomic monitoringBrazilGenomaBrasilEquinoVírus do Nilo OcidentalsettingsOrder Article Reprints Open AccessBrief Report Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil by Hegger Fritsch 1,†,Felicidade Mota Pereira 2,†,Erica Azevedo Costa 1,†ORCID,Vagner Fonseca 3,†ORCID,Stephane Tosta 1,†,Joilson Xavier 1ORCID,Flavia Levy 4,Carla de Oliveira 4,Gabriela Menezes 2,Jaqueline Lima 2,Lenisa Santos 2,Luciana Silva 2,Vanessa Nardy 2,Marcela Kelly Gómez Astete 2ORCID,Beatriz Senra Álvares da Silva Santos 1ORCID,Nágila Rocha Aguiar 1,Maria Isabel Maldonado Coelho Guedes 1ORCID,Guilherme Canhestro de Faria 5,Ronaldo Furtini 5,Safira Rachel Milanez Drumond 5,add Show full author list 1 Laboratorio de Pesquisa em Virologia Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil 2 Laboratório Central de Saúde Pública Prof Goncalo Moniz, Salvador 41745-900, Brazil 3 Organização Pan-Americana de Saúde/Organização Mundial de Saúde, Brasilia 37650-000, Brazil 4 Laboratorio de Flavivirus, lnstituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil 5 Laboratório de Saúde Animal, Instituto Mineiro de Agropecuária, Belo Horizonte 30110-005, Brazil 6 Secretary of Health of the State of Bahia (SESAB), Salvador 40301-110, Brazil 7 Coordenação Geral dos Laboratórios de Saúde Pública, Secretaria de Vigilância em Saúde-Brazil, Brasília 70719-040, Brazil 8 Coordenação Geral das Arboviroses, Secretaria de Vigilância em Saúde (CGARB/SVS-MS), Brasilia 37650-000, Brazil 9 Fundação Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro 21040-360, Brazil 10 Department of Science and Technology for Humans and the Environment, University of Campus Bio-Medico, 00128 Rome, Italy * Authors to whom correspondence should be addressed. † These authors contributed equally to this work. Viruses 2022, 14(7), 1540; https://doi.org/10.3390/v14071540 Submission received: 17 June 2022 / Revised: 10 July 2022 / Accepted: 11 July 2022 / Published: 14 July 2022 (This article belongs to the Special Issue Usutu, West Nile and Tickborne Encephalitis Viruses) Downloadkeyboard_arrow_down Browse Figure Versions Notes Abstract During these past years, several studies have provided serological evidence regarding the circulation of West Nile virus (WNV) in Brazil. Despite some reports, much is still unknown regarding the genomic diversity and transmission dynamics of this virus in the country. Recently, genomic monitoring activities in horses revealed the circulation of WNV in several Brazilian regions. These findings on the paucity of genomic data reinforce the need for prompt investigation of WNV infection in horses, which may precede human cases of encephalitis in Brazil. Thus, in this study, we retrospectively screened 54 suspicious WNV samples collected between 2017 and 2020 from the spinal cord and brain of horses with encephalitis and generated three new WNV genomes from the Ceará and Bahia states, located in the northeastern region of Brazil. The Bayesian reconstruction revealed that at least two independent introduction events occurred in Brazil. The first introduction event appears to be likely related to the North American outbreak, and was estimated to have occurred in March 2013.The second introduction event appears to have occurred in September 2017 and appears to be likely related to the South American outbreak. Together, our results reinforce the importance of increasing the priority of WNV genomic monitoring in equines with encephalitis in order to track the dispersion of this emerging pathogen through the country.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFAPERJ - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroUniversidade Federal de Minas GeraisBrasilVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVAVETER - ESCOLA DE VETERINARIAUFMG2024-04-23T14:42:46Z2024-04-23T14:42:46Z2022-07-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdfhttps://doi.org/10.3390/v140715401999-4915http://hdl.handle.net/1843/67591https://orcid.org/0000-0002-5396-3818https://orcid.org/0000-0001-5521-6448https://orcid.org/0000-0003-4426-6989https://orcid.org/0000-0003-3916-2018https://orcid.org/0000-0002-1497-4397https://orcid.org/0000-0002-6734-0216https://orcid.org/0000-0002-7684-2883https://orcid.org/0000-0002-5849-7326https://orcid.org/0000-0002-6769-9931engVirusesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGHegger FritschJaqueline LimaLenisa SantosLuciana SilvaVanessa NardyMarcela Kelly Gómez AsteteBeatriz Senra Álvares da Silva SantosNágila Rocha AguilarMaria Isabel Maldonado Coelho GuedesGuilherme Canhestro de FariaRonaldo FurtiniFelicidade Mota PereiraSafira Rachel Milanez DrumondGabriel Muricy CunhaMarcia São Pedro Leal SouzaRonaldo de JesusSara A. Franco GuimarãesItalo Coelho NunoIan Carlos Brito de SantanaJosé Eduardo Ungar de sáGeorge Roma SantosWilladesmon Santos SilvaErica Azevedo CostaThiago Ferreira GuedesEmerson Luiz Lima AraújoRodrigo Fabiano do Carmo SaidCarlos Frederico Campelo de AlbuquerqueCassio Roberto Leonel PeterkaAlessandro Pecego Martins RomanoRivaldo Venâncio da CunhaAna Maria Bispo de FilippisArabela Leal e Silva de MelloMarta GiovanettiVagner FonsecaLuiz Carlos Junior AlcantaraStephane TostaJoilson XavierFlavia LevyCarla de OliveiraGabriela Menezes2024-04-23T14:42:47Zoai:repositorio.ufmg.br:1843/67591Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2024-04-23T14:42:47Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
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Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil

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