Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum.
Coniothurium minitans (CM) is hyperparasitic to Sclerotinia sderotiorum (55), a palhogen of many economically important crops. In this paper, we describe lhe isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Brasil |
| Institución: | Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
| Repositorio: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
| Idioma: | inglés |
| OAI Identifier: | oai:www.alice.cnptia.embrapa.br:doc/898276 |
| Acceso en línea: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/898276 |
| Access Level: | acceso abierto |
| Palabra clave: | Coniothvrium minitans Resistance Antibiotic production Sclerotinia sclerotiorum Doença fúngica Controle biológico Fungo para controle biológico Biological control Antifungal agents iprodione |
| Sumario: | Coniothurium minitans (CM) is hyperparasitic to Sclerotinia sderotiorum (55), a palhogen of many economically important crops. In this paper, we describe lhe isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effect:iveagainst Ss. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80%of sclerotia were colonized by lhe mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed frorn their wild-type strain in appearance, morphology and sporulation. In conclusion, the results presented here provide evidence that lhe new biotypes of C. minitans are effective in controlling S.sclemtiarum. |
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