CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN

A high molecular weight chondroitin sulfate proteoglycan (M(r) 240,000) is released from platelet surface during aggregation induced by several pharmacological agents. Some details on the structure of this compound are reported. Beta-elimination with alkali and borohydride produces chondroitin sulfa...

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Detalles Bibliográficos
Autor: Nader, Helena Bonciani [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1991
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/44197
Acceso en línea:http://www.jbc.org/content/266/16/10518.abstract
http://repositorio.unifesp.br/handle/11600/44197
Access Level:acceso abierto
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spelling CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCANA high molecular weight chondroitin sulfate proteoglycan (M(r) 240,000) is released from platelet surface during aggregation induced by several pharmacological agents. Some details on the structure of this compound are reported. Beta-elimination with alkali and borohydride produces chondroitin sulfate chains with a molecular weight of 40,000. The combined results indicate a proteoglycan molecule containing 5-6 chondroitin sulfate chains and a protein core rich in serine and glycine residues. Degradation with chondroitinase AC shows that a 4-sulfated disaccharide is the only disaccharide released from this chondroitin sulfate, characterizing it as a chondroitin 4-sulfate homopolymer. It is shown that this proteoglycan inhibits the aggregation of platelets induced by ADP. Analysis of the sulfated glycosaminoglycans not released during aggregation revealed the presence of a heparan sulfate in the platelets. Degradation by heparitinases I and II yielded the four disaccharide units of heparan sulfates: N,O-disulfated disaccharide, N-sulfated disaccharide, N-acetylated 6-sulfated disaccharide, and N-acetylated disaccharide. The possible role of the sulfated glycosaminoglycans on cell-cell interaction is discussed in view of the present findings.ESCOLA PAULISTA MED SCH,DEPT BIOQUIM,CAIXA POSTAL 20372,BR-04023 SAO PAULO,BRAZILESCOLA PAULISTA MED SCH,DEPT BIOQUIM,CAIXA POSTAL 20372,BR-04023 SAO PAULO,BRAZILWeb of ScienceAmer Soc Biochemistry Molecular Biology IncUniversidade Federal de São Paulo (UNIFESP)2018-06-15T17:53:04Z2018-06-15T17:53:04Z1991-06-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10518-10523http://www.jbc.org/content/266/16/10518.abstractJournal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 266, n. 16, p. 10518-10523, 1991.0021-9258http://repositorio.unifesp.br/handle/11600/44197WOS:A1991FP08600079ark:/48912/0013000022n5tengJournal Of Biological Chemistryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPNader, Helena Bonciani [UNIFESP]2024-05-02T11:58:49Zoai:repositorio.unifesp.br:11600/44197Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T11:58:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
title CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
spellingShingle CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
Nader, Helena Bonciani [UNIFESP]
title_short CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
title_full CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
title_fullStr CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
title_full_unstemmed CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
title_sort CHARACTERIZATION OF A HEPARAN-SULFATE AND A PECULIAR CHONDROITIN 4-SULFATE PROTEOGLYCAN FROM PLATELETS - INHIBITION OF THE AGGREGATION PROCESS BY PLATELET CHONDROITIN SULFATE PROTEOGLYCAN
dc.creator.none.fl_str_mv Nader, Helena Bonciani [UNIFESP]
author Nader, Helena Bonciani [UNIFESP]
author_facet Nader, Helena Bonciani [UNIFESP]
author_role author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
description A high molecular weight chondroitin sulfate proteoglycan (M(r) 240,000) is released from platelet surface during aggregation induced by several pharmacological agents. Some details on the structure of this compound are reported. Beta-elimination with alkali and borohydride produces chondroitin sulfate chains with a molecular weight of 40,000. The combined results indicate a proteoglycan molecule containing 5-6 chondroitin sulfate chains and a protein core rich in serine and glycine residues. Degradation with chondroitinase AC shows that a 4-sulfated disaccharide is the only disaccharide released from this chondroitin sulfate, characterizing it as a chondroitin 4-sulfate homopolymer. It is shown that this proteoglycan inhibits the aggregation of platelets induced by ADP. Analysis of the sulfated glycosaminoglycans not released during aggregation revealed the presence of a heparan sulfate in the platelets. Degradation by heparitinases I and II yielded the four disaccharide units of heparan sulfates: N,O-disulfated disaccharide, N-sulfated disaccharide, N-acetylated 6-sulfated disaccharide, and N-acetylated disaccharide. The possible role of the sulfated glycosaminoglycans on cell-cell interaction is discussed in view of the present findings.
publishDate 1991
dc.date.none.fl_str_mv 1991-06-05
2018-06-15T17:53:04Z
2018-06-15T17:53:04Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.jbc.org/content/266/16/10518.abstract
Journal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 266, n. 16, p. 10518-10523, 1991.
0021-9258
http://repositorio.unifesp.br/handle/11600/44197
WOS:A1991FP08600079
dc.identifier.dark.fl_str_mv ark:/48912/0013000022n5t
url http://www.jbc.org/content/266/16/10518.abstract
http://repositorio.unifesp.br/handle/11600/44197
identifier_str_mv Journal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 266, n. 16, p. 10518-10523, 1991.
0021-9258
WOS:A1991FP08600079
ark:/48912/0013000022n5t
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Biological Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10518-10523
dc.publisher.none.fl_str_mv Amer Soc Biochemistry Molecular Biology Inc
publisher.none.fl_str_mv Amer Soc Biochemistry Molecular Biology Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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score 15,301603

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