Mapping biochemical pathways in maytenus ilicifolia (celastraceae) through integrated proteomics and histochemistry
Maytenus ilicifolia (Celastraceae) is a medicinal plant that is native to southern Brazil and is popularly known as espinheira-santa. From a biosynthesis perspective, this species accumulates quinonemethide triterpenes and sesquiterpene pyridine alkaloids as major secondary metabolites that exhibit...
| Autores: | , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/208314 |
| Acesso em linha: | http://dx.doi.org/10.21577/0103-5053.20200173 http://hdl.handle.net/11449/208314 |
| Access Level: | acceso abierto |
| Palavra-chave: | Celastraceae Histochemical analysis Maytenus ilicifolia Quinonemethide triterpenes Sesquiterpene pyridine alkaloids Shotgun proteomic approach |
| Resumo: | Maytenus ilicifolia (Celastraceae) is a medicinal plant that is native to southern Brazil and is popularly known as espinheira-santa. From a biosynthesis perspective, this species accumulates quinonemethide triterpenes and sesquiterpene pyridine alkaloids as major secondary metabolites that exhibit interesting biological properties, with antitumoral and antiprotozoal activities, respectively, being the most frequently reported. Additionally, the restricted accumulation of such compounds in the roots raises questions about the expression of proteins involved in such compartmentalization and their possible biological and/or ecological role in M. ilicifolia. Thus, this article describes the use of shotgun proteomics and histochemical studies for the characterization of the main biosynthetic pathways involved in the regulation of the metabolism in M. ilicifolia roots. This combined approaches also resulted in the identification of a series of proteins involved in the quinonemethide triterpenes and sesquiterpene pyridine alkaloids, providing evidences of their differential compartmentalization |
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