Polycomb & Trithorax uma análise da expressão gênica do cérebro de camundongos fêmeas obesos transplantados com tecido adiposo gonadal de fêmeas magros
The Polycystic Ovary Syndrome (PCOS) is the most common androgenic disorder in women during reproductive life. PCOS may also be accompanied by metabolic syndrome and recent studies points to leptin as playing a role in disrupting infertility and in changing the energy balance in obese mice through i...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Brasil |
| Institución: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/50949 |
| Acceso en línea: | https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5766150 http://repositorio.unifesp.br/handle/11600/50949 |
| Access Level: | acceso abierto |
| Palabra clave: | Polycistic Ovarian Sindrome Obesity Polycomb and trithorax complexes Leptin Fat female mouse xxiii Drosophila proteins Disease models, animal Sindrome do ovário policístico Obesidade Complexos polycomb e trithorax Leptina Camundongo fêmea obeso Proteínas de drosophila Proteínas do grupo polycomb Modelos animais de doenças |
| Sumario: | The Polycystic Ovary Syndrome (PCOS) is the most common androgenic disorder in women during reproductive life. PCOS may also be accompanied by metabolic syndrome and recent studies points to leptin as playing a role in disrupting infertility and in changing the energy balance in obese mice through its action on the hypothalamus. The aim is to assess the expression of the Polycomb & Trithorax Complexes genes in the brain of mice, transplanted with fat tissue from normal mice, to better understand the neuronal molecular mechanisms underlying the reversion of PCOS. Brain material of four B6 V-Lepob/J mouse groups is used: norma! weight, obese (control), 7 days treated obese and 45 days treaded obese. AH have their brain RNA extracted and submitted to an 84 Polycomb & Trithorax complexes genes PCR array plate and MetaCore™ software for canonical pathways localization. Genomic profiles obtained are compared to the ones of the control group and presented differentially expressed genes of 25% and 10,7% at 7 and 45 days, respectively, after the treatment. At 7 days, 90,5% presented hyper-regulated and 9,5% hypo-regulated and, finally, at 45 days 100% presented hyper-regulated. The major changes occur in genes: Snail l 36,1; Smarcal l -14,3; Ctbp2 l 5,7; Ezh1 i 5,65; Rnasel l 5,51 and Dnml3b l 5,50. Expressly-altered genes are associated to canonical pathways and provide three networks related to epigenetics. Underlying neuronal changes caused by íeptin in obese-mice brain, there is an important role played by the histone code. Here is shown evidence that leptin drives the chromatin packing to a more condensed pattern. Upregulation of methyltransferase genes, like Ezh1, favors this thought. In summary, the Polycomb & Trithorax complexes might answer for the silencing of some downregulated genes in the obese mice brain when exposed to leptin. |
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