Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.

Forage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea a...

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Autores: NUNES, G. F. de O., MENEZES, K. A. S., SAMPAIO, A. A., LEITE, J., FERNANDES JUNIOR, P. I., SEIDO, S. L., ZILLI, J. E., MARTINS, L. M. V.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Repositorio:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Idioma:portugués
OAI Identifier:oai:www.alice.cnptia.embrapa.br:doc/1094257
Acceso en línea:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094257
http://dx.doi.org/10.19084/RCA17339
Access Level:acceso abierto
Palabra clave:Fixação biológica do nitrogênio
Controle Biológico
Fixação de Nitrogênio
Clitoria Ternatea
Leguminosa Forrageira
Biological control
Stylosanthes capitata
Bradyrhizobium
Nitrogen fixation
Forage legumes
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spelling Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.Fixação biológica do nitrogênioControle BiológicoFixação de NitrogênioClitoria TernateaLeguminosa ForrageiraBiological controlStylosanthes capitataBradyrhizobiumNitrogen fixationForage legumesForage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically di verse bacteria inhabiting the nodules of the forage legumes C. tematea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions.GERSIKA FAKIRRA DE OLIVEIRA NUNES, UNIVERSIDADE DO ESTADO DA BAHIAKELLY ALEXSANDRA SOUZA MENEZES, UNIVERSIDADE FEDERAL DE ALAGOASALINE ARAÚJO SAMPAIO, UNIVERSIDADE FEDERAL DA BAHIAJAKSON LEITE, UNIVERSIDADE FEDERAL DE ALAGOASPAULO IVAN FERNANDES JUNIOR, CPATSASIRANDO LIMA SEIDO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCOJERRI EDSON ZILLI, CNPABLINDETE MÍRIA VIEIRA MARTINS, UNIVERSIDADE DO ESTADO DA BAHIA.2018-08-21T00:53:15Z2018-08-21T00:53:15Z2018-08-2020182018-08-22T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista de Ciências Agrárias, v. 41, n. 3, p. 612-624, jul./set. 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094257http://dx.doi.org/10.19084/RCA17339porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPANUNES, G. F. de O.MENEZES, K. A. S.SAMPAIO, A. A.LEITE, J.FERNANDES JUNIOR, P. I.SEIDO, S. L.ZILLI, J. E.MARTINS, L. M. V.2018-08-21T00:53:22Zoai:www.alice.cnptia.embrapa.br:doc/1094257Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-08-21T00:53:22Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
title Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
spellingShingle Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
NUNES, G. F. de O.
Fixação biológica do nitrogênio
Controle Biológico
Fixação de Nitrogênio
Clitoria Ternatea
Leguminosa Forrageira
Biological control
Stylosanthes capitata
Bradyrhizobium
Nitrogen fixation
Forage legumes
title_short Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
title_full Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
title_fullStr Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
title_full_unstemmed Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
title_sort Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.
dc.creator.none.fl_str_mv NUNES, G. F. de O.
MENEZES, K. A. S.
SAMPAIO, A. A.
LEITE, J.
FERNANDES JUNIOR, P. I.
SEIDO, S. L.
ZILLI, J. E.
MARTINS, L. M. V.
author NUNES, G. F. de O.
author_facet NUNES, G. F. de O.
MENEZES, K. A. S.
SAMPAIO, A. A.
LEITE, J.
FERNANDES JUNIOR, P. I.
SEIDO, S. L.
ZILLI, J. E.
MARTINS, L. M. V.
author_role author
author2 MENEZES, K. A. S.
SAMPAIO, A. A.
LEITE, J.
FERNANDES JUNIOR, P. I.
SEIDO, S. L.
ZILLI, J. E.
MARTINS, L. M. V.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv GERSIKA FAKIRRA DE OLIVEIRA NUNES, UNIVERSIDADE DO ESTADO DA BAHIA
KELLY ALEXSANDRA SOUZA MENEZES, UNIVERSIDADE FEDERAL DE ALAGOAS
ALINE ARAÚJO SAMPAIO, UNIVERSIDADE FEDERAL DA BAHIA
JAKSON LEITE, UNIVERSIDADE FEDERAL DE ALAGOAS
PAULO IVAN FERNANDES JUNIOR, CPATSA
SIRANDO LIMA SEIDO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO
JERRI EDSON ZILLI, CNPAB
LINDETE MÍRIA VIEIRA MARTINS, UNIVERSIDADE DO ESTADO DA BAHIA.
dc.subject.por.fl_str_mv Fixação biológica do nitrogênio
Controle Biológico
Fixação de Nitrogênio
Clitoria Ternatea
Leguminosa Forrageira
Biological control
Stylosanthes capitata
Bradyrhizobium
Nitrogen fixation
Forage legumes
topic Fixação biológica do nitrogênio
Controle Biológico
Fixação de Nitrogênio
Clitoria Ternatea
Leguminosa Forrageira
Biological control
Stylosanthes capitata
Bradyrhizobium
Nitrogen fixation
Forage legumes
description Forage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically di verse bacteria inhabiting the nodules of the forage legumes C. tematea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-21T00:53:15Z
2018-08-21T00:53:15Z
2018-08-20
2018
2018-08-22T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista de Ciências Agrárias, v. 41, n. 3, p. 612-624, jul./set. 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094257
http://dx.doi.org/10.19084/RCA17339
identifier_str_mv Revista de Ciências Agrárias, v. 41, n. 3, p. 612-624, jul./set. 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094257
http://dx.doi.org/10.19084/RCA17339
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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