Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.

To determine the role of the spleen in the pathogenesis of canine visceral leishmaniasis (CVL), we analyzed cellular immunophenotypic profiles of 52 dogs naturally infected with Leishmania infantum, clinically classified as follows: asymptomatic dogs-I (AD-I), seroneg-ative/PCR+; asymptomatic dogs-I...

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Autores: Reis, Alexandre Barbosa, Carvalho, Andréa Teixeira de, Giunchetti, Rodolfo Cordeiro, Roatt, Bruno Mendes, Vital, Wendel Coura, Nicolato, Roney Luiz de Carvalho, Lemos, Denise da Silveira, Oliveira, Rodrigo Corrêa de, Martins Filho, Olindo Assis
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Recursos:Universidade Federal de Ouro Preto (UFOP)
Repositorio:Repositório Institucional da UFOP
Idioma:inglés
OAI Identifier:oai:repositorio.ufop.br:123456789/4266
Acesso em linha:http://www.repositorio.ufop.br/handle/123456789/4266
https://doi.org/10.1016/j.vetimm.2013.11.005
Access Level:acceso abierto
Palavra-chave:Leishmania infantum
Canine visceral leishmaniasis
Splenic compartment
Cellular immune response
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spelling Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.Leishmania infantumCanine visceral leishmaniasisSplenic compartmentCellular immune responseTo determine the role of the spleen in the pathogenesis of canine visceral leishmaniasis (CVL), we analyzed cellular immunophenotypic profiles of 52 dogs naturally infected with Leishmania infantum, clinically classified as follows: asymptomatic dogs-I (AD-I), seroneg-ative/PCR+; asymptomatic dogs-II (AD-II), seropositive/PCR+; oligosymptomatic dogs (OD) and symptomatic dogs (SD). Seven non-infected dogs (CD) were included as a control group. AD-II presented higher levels of CD8+ T splenocytes and lower TCD4+/TCD8+ ratio in com-parison with CD. OD and SD showed lower percentages of CD21+ as compared with AD-II. All seropositive dogs presented lower levels of CD45RA+ than CD. Regardless of the stimuli used, the proliferation index from splenocytes in vitro was inversely correlated with clini-cal status. After LSA stimulation, there was a higher percentage of specific CD8+ T in AD-II than CD and non-stimulated culture. In contrast, splenocytes from SD under in vitro LSA stimulation induced decreased MHC-II+ expression in comparison with all groups, and non-stimulated culture. In conclusion, the role of CD8+ T splenocytes seems to be important for an effective immunological response, a hallmark of asymptomatic CVL, whereas the pro-nounced loss of MHC-II expression upon LSA stimulation is a biomarker of symptomatic CVL.2015-01-19T16:52:06Z2015-01-19T16:52:06Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfREIS, A. B. et al. Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 157, p. 190-196, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0165242713003115>. Acesso em: 13 ago. 2014.0165-2427http://www.repositorio.ufop.br/handle/123456789/4266https://doi.org/10.1016/j.vetimm.2013.11.005ark:/61566/0013000006znhO periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3442010775996.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPReis, Alexandre BarbosaCarvalho, Andréa Teixeira deGiunchetti, Rodolfo CordeiroRoatt, Bruno MendesVital, Wendel CouraNicolato, Roney Luiz de CarvalhoLemos, Denise da SilveiraOliveira, Rodrigo Corrêa deMartins Filho, Olindo Assis2024-11-10T18:12:36Zoai:repositorio.ufop.br:123456789/4266Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332024-11-10T18:12:36Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
title Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
spellingShingle Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
Reis, Alexandre Barbosa
Leishmania infantum
Canine visceral leishmaniasis
Splenic compartment
Cellular immune response
title_short Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
title_full Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
title_fullStr Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
title_full_unstemmed Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
title_sort Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
dc.creator.none.fl_str_mv Reis, Alexandre Barbosa
Carvalho, Andréa Teixeira de
Giunchetti, Rodolfo Cordeiro
Roatt, Bruno Mendes
Vital, Wendel Coura
Nicolato, Roney Luiz de Carvalho
Lemos, Denise da Silveira
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
author Reis, Alexandre Barbosa
author_facet Reis, Alexandre Barbosa
Carvalho, Andréa Teixeira de
Giunchetti, Rodolfo Cordeiro
Roatt, Bruno Mendes
Vital, Wendel Coura
Nicolato, Roney Luiz de Carvalho
Lemos, Denise da Silveira
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
author_role author
author2 Carvalho, Andréa Teixeira de
Giunchetti, Rodolfo Cordeiro
Roatt, Bruno Mendes
Vital, Wendel Coura
Nicolato, Roney Luiz de Carvalho
Lemos, Denise da Silveira
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
author2_role author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Leishmania infantum
Canine visceral leishmaniasis
Splenic compartment
Cellular immune response
topic Leishmania infantum
Canine visceral leishmaniasis
Splenic compartment
Cellular immune response
description To determine the role of the spleen in the pathogenesis of canine visceral leishmaniasis (CVL), we analyzed cellular immunophenotypic profiles of 52 dogs naturally infected with Leishmania infantum, clinically classified as follows: asymptomatic dogs-I (AD-I), seroneg-ative/PCR+; asymptomatic dogs-II (AD-II), seropositive/PCR+; oligosymptomatic dogs (OD) and symptomatic dogs (SD). Seven non-infected dogs (CD) were included as a control group. AD-II presented higher levels of CD8+ T splenocytes and lower TCD4+/TCD8+ ratio in com-parison with CD. OD and SD showed lower percentages of CD21+ as compared with AD-II. All seropositive dogs presented lower levels of CD45RA+ than CD. Regardless of the stimuli used, the proliferation index from splenocytes in vitro was inversely correlated with clini-cal status. After LSA stimulation, there was a higher percentage of specific CD8+ T in AD-II than CD and non-stimulated culture. In contrast, splenocytes from SD under in vitro LSA stimulation induced decreased MHC-II+ expression in comparison with all groups, and non-stimulated culture. In conclusion, the role of CD8+ T splenocytes seems to be important for an effective immunological response, a hallmark of asymptomatic CVL, whereas the pro-nounced loss of MHC-II expression upon LSA stimulation is a biomarker of symptomatic CVL.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015-01-19T16:52:06Z
2015-01-19T16:52:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv REIS, A. B. et al. Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 157, p. 190-196, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0165242713003115>. Acesso em: 13 ago. 2014.
0165-2427
http://www.repositorio.ufop.br/handle/123456789/4266
https://doi.org/10.1016/j.vetimm.2013.11.005
dc.identifier.dark.fl_str_mv ark:/61566/0013000006znh
identifier_str_mv REIS, A. B. et al. Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 157, p. 190-196, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0165242713003115>. Acesso em: 13 ago. 2014.
0165-2427
ark:/61566/0013000006znh
url http://www.repositorio.ufop.br/handle/123456789/4266
https://doi.org/10.1016/j.vetimm.2013.11.005
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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score 15,198674

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