One Health genomic surveillance of antibiotic-resistant nontyphoidal Salmonella enterica serovars in Brazil
Nontyphoidal Salmonella (NTS) is one of the most frequently reported pathogens responsible for foodborne outbreaks worldwide, whose transmission involves various pathways within the One Health intersection. Currently, the emergence of antimicrobial resistance (AMR) poses a significant global health...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | Brasil |
| Institución: | Universidade de São Paulo (USP) |
| Repositorio: | Biblioteca Digital de Teses e Dissertações da USP |
| Idioma: | inglés |
| OAI Identifier: | oai:teses.usp.br:tde-10102024-143136 |
| Acceso en línea: | https://www.teses.usp.br/teses/disponiveis/9/9136/tde-10102024-143136/ |
| Access Level: | acceso abierto |
| Palabra clave: | Antimicrobial resistance Nontyphoidal Salmonella One Health Patógeno zoonótico Resistência antimicrobiana Salmonella não tifóide Saúde Única Sequenciamento genoma completo Wholegenome sequencing Zoonotic pathogen |
| Sumario: | Nontyphoidal Salmonella (NTS) is one of the most frequently reported pathogens responsible for foodborne outbreaks worldwide, whose transmission involves various pathways within the One Health intersection. Currently, the emergence of antimicrobial resistance (AMR) poses a significant global health threat by limiting therapeutic options to treat various infections. This study aimed to establish the first Salmonella enterica Brazilian Database (SeBR) by providing clinically relevant genomic information of nontyphoidal Salmonella serovars from Brazil, and to perform comparative analysis to shed light into the current epidemiology trends by identifying serovars, sequence types (ST), resistome, plasmidome and virulence of antimicrobialresistant NTS. The Salmonella strains subject to whole-genome sequencing were recovered from different Brazilian states over a period of 25-year (1995-2020), which belonged to distinct serovars and sequence types (ST), including serovars Heidelberg (ST15), Minnesota (ST548, ST3088), Muenchen (ST112), Typhimurium (ST19, ST313), Newport (ST118), Enteritidis (ST19), Javiana (ST1674, ST2783), Coeln (ST2015), Infantis (ST32), Matadi (ST973), and Panama (ST48). Bioinformatic analysis revealed resistance genes against critical antimicrobials for the treatment of salmonellosis, including beta-lactams (blaCMY, blaCTX-M, blaSHV, blaKPC), fluoroquinolones (qnrB19, qnrS1), and macrolides (mphA, mphB). Noteworthy, S. Heidelberg ST15, S. Minnesota ST548 and S. Muenchen ST112 were the most prevalent One Health serovars, being isolated from human samples, food products, animals and environmental sources. On the other hand, S. Typhimurium, S. Javiana, S. Enteritidis, S. Coeln, S. Infantis were only found in humans. A wide virulome and plasmid replicon diversity were predicted in all serotypes. These findings highlight the constant need for genomic surveillance of priority pathogens in order to monitor resistance trends and develop strategies for AMR mitigation. |
|---|