KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT....

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Bibliographic Details
Authors: Caiaffa, Karina Sampaio [UNESP], Massunari, Loiane [UNESP], Danelon, Marcelle [UNESP], Abuna, Gabriel Flores, Bedran, Telma Blanca Lombardo [UNESP], Santos-Filho, Norival Alves [UNESP], Spolidorio, Denise Madalena Palomari [UNESP], Vizoto, Natalia Leal, Cilli, Eduardo Maffud [UNESP], Duque, Cristiane [UNESP]
Format: article
Status:Published version
Publication Date:2017
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/175339
Online Access:http://dx.doi.org/10.1080/08927014.2017.1370087
http://hdl.handle.net/11449/175339
Access Level:Open access
Keyword:Biofilms
cationic antimicrobial peptides
cell culture
microbial sensitivity tests
Description
Summary:This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml−1, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections.

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