Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage
Fetomaternal hemorrhage, characterized by the passage of fetal blood into maternal circulation, poses significant risks to both mother and fetus. As it may be diagnosed by quantifying fetal red blood cells in maternal circulation, we developed an immunosensor to detect fetal cells made with a layer-...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2025 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositório: | Repositório Institucional da UNESP |
| Idioma: | inglês |
| OAI Identifier: | oai:repositorio.unesp.br:11449/300547 |
| Acesso em linha: | http://dx.doi.org/10.1016/j.bioelechem.2025.108983 https://hdl.handle.net/11449/300547 |
| Access Level: | Acceso aberto |
| Palavra-chave: | CD71 Electrical impedance spectroscopy Fetomaternal hemorrhage Gold interdigitated electrode Immunosensor Silk fibroin |
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Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhageCD71Electrical impedance spectroscopyFetomaternal hemorrhageGold interdigitated electrodeImmunosensorSilk fibroinFetomaternal hemorrhage, characterized by the passage of fetal blood into maternal circulation, poses significant risks to both mother and fetus. As it may be diagnosed by quantifying fetal red blood cells in maternal circulation, we developed an immunosensor to detect fetal cells made with a layer-by-layer silk fibroin film and a commercial CD71 monoclonal antibody. Detection was achieved through electrical impedance spectroscopy, allowing discrimination between human umbilical cord blood (UCB) cells and adult peripheral blood (APB) cells from patients and volunteers. Positive samples (UCB) exhibited an average capacitance of 0.34 nF at 1 to 10 Hz, while negative samples (APB) had 0.05 nF. Full agreement was obtained with the detection of fetal red blood cells using flow cytometry. The change in the electrical response was associated with the adsorption of UCB cells on the immunosensor, primarily due to conformational changes in the amide I groups. This reflects the immunoaffinity interactions, as revealed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). Cell adsorption was confirmed with optical microscopy images, which indicate possible point-of-care strategies to diagnose FMH with image analysis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) Medical School, SPSão Paulo State University (UNESP) School of Pharmaceutical Sciences, SPSão Paulo State University (UNESP) Clinical Hospital of Botucatu Medical School, SPFederal University of São Paulo (UNIFESP) Institute of Science and Technology, SPSão Paulo State University (UNESP) Institute of Biosciences, SPSão Paulo State University (UNESP) School of Agricultural Sciences, SPUniversity of Sao Paulo (USP) Sao Carlos Institute of Physics, SPSão Paulo State University (UNESP) Institute of Chemistry, SPSão Paulo State University (UNESP) Medical School, SPSão Paulo State University (UNESP) School of Pharmaceutical Sciences, SPSão Paulo State University (UNESP) Clinical Hospital of Botucatu Medical School, SPSão Paulo State University (UNESP) Institute of Biosciences, SPSão Paulo State University (UNESP) School of Agricultural Sciences, SPSão Paulo State University (UNESP) Institute of Chemistry, SPFAPESP: 2018/22214-6Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)2025-04-29T18:49:54Z2025-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bioelechem.2025.108983Bioelectrochemistry, v. 165.1878-562X1567-5394https://hdl.handle.net/11449/30054710.1016/j.bioelechem.2025.1089832-s2.0-105002285923Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioelectrochemistryinfo:eu-repo/semantics/openAccessAssunpção Nishio, Suelen [UNESP]Moroz, Andrei [UNESP]de Assis Golim, Marjorie [UNESP]Braz, Aline Marcia Marques [UNESP]Yokomichi, Anna Laura YuriPedrosa, Valber A. [UNESP]Cesarino, Ivana [UNESP]Oliveira Jr., Osvaldo N.Ribeiro, Sidney J.L. [UNESP]Deffune, Elenice [UNESP]Moraes, Marli Leite de2025-10-16T12:37:15Zoai:repositorio.unesp.br:11449/300547Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-16T12:37:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage |
| title |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage |
| spellingShingle |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage Assunpção Nishio, Suelen [UNESP] CD71 Electrical impedance spectroscopy Fetomaternal hemorrhage Gold interdigitated electrode Immunosensor Silk fibroin |
| title_short |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage |
| title_full |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage |
| title_fullStr |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage |
| title_full_unstemmed |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage |
| title_sort |
Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage |
| dc.creator.none.fl_str_mv |
Assunpção Nishio, Suelen [UNESP] Moroz, Andrei [UNESP] de Assis Golim, Marjorie [UNESP] Braz, Aline Marcia Marques [UNESP] Yokomichi, Anna Laura Yuri Pedrosa, Valber A. [UNESP] Cesarino, Ivana [UNESP] Oliveira Jr., Osvaldo N. Ribeiro, Sidney J.L. [UNESP] Deffune, Elenice [UNESP] Moraes, Marli Leite de |
| author |
Assunpção Nishio, Suelen [UNESP] |
| author_facet |
Assunpção Nishio, Suelen [UNESP] Moroz, Andrei [UNESP] de Assis Golim, Marjorie [UNESP] Braz, Aline Marcia Marques [UNESP] Yokomichi, Anna Laura Yuri Pedrosa, Valber A. [UNESP] Cesarino, Ivana [UNESP] Oliveira Jr., Osvaldo N. Ribeiro, Sidney J.L. [UNESP] Deffune, Elenice [UNESP] Moraes, Marli Leite de |
| author_role |
author |
| author2 |
Moroz, Andrei [UNESP] de Assis Golim, Marjorie [UNESP] Braz, Aline Marcia Marques [UNESP] Yokomichi, Anna Laura Yuri Pedrosa, Valber A. [UNESP] Cesarino, Ivana [UNESP] Oliveira Jr., Osvaldo N. Ribeiro, Sidney J.L. [UNESP] Deffune, Elenice [UNESP] Moraes, Marli Leite de |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
| dc.subject.por.fl_str_mv |
CD71 Electrical impedance spectroscopy Fetomaternal hemorrhage Gold interdigitated electrode Immunosensor Silk fibroin |
| topic |
CD71 Electrical impedance spectroscopy Fetomaternal hemorrhage Gold interdigitated electrode Immunosensor Silk fibroin |
| description |
Fetomaternal hemorrhage, characterized by the passage of fetal blood into maternal circulation, poses significant risks to both mother and fetus. As it may be diagnosed by quantifying fetal red blood cells in maternal circulation, we developed an immunosensor to detect fetal cells made with a layer-by-layer silk fibroin film and a commercial CD71 monoclonal antibody. Detection was achieved through electrical impedance spectroscopy, allowing discrimination between human umbilical cord blood (UCB) cells and adult peripheral blood (APB) cells from patients and volunteers. Positive samples (UCB) exhibited an average capacitance of 0.34 nF at 1 to 10 Hz, while negative samples (APB) had 0.05 nF. Full agreement was obtained with the detection of fetal red blood cells using flow cytometry. The change in the electrical response was associated with the adsorption of UCB cells on the immunosensor, primarily due to conformational changes in the amide I groups. This reflects the immunoaffinity interactions, as revealed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). Cell adsorption was confirmed with optical microscopy images, which indicate possible point-of-care strategies to diagnose FMH with image analysis. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025-04-29T18:49:54Z 2025-10-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bioelechem.2025.108983 Bioelectrochemistry, v. 165. 1878-562X 1567-5394 https://hdl.handle.net/11449/300547 10.1016/j.bioelechem.2025.108983 2-s2.0-105002285923 |
| url |
http://dx.doi.org/10.1016/j.bioelechem.2025.108983 https://hdl.handle.net/11449/300547 |
| identifier_str_mv |
Bioelectrochemistry, v. 165. 1878-562X 1567-5394 10.1016/j.bioelechem.2025.108983 2-s2.0-105002285923 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Bioelectrochemistry |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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