Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni

Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)

Detalles Bibliográficos
Autores: Pereira, Thiago de Almeida, Syn, Wing-Kin, Machado, Mariana V., Vidigal, Paula Vieira Teixeira, Resende, Vivian, Voieta, Izabela, Xie, Guanhua, Otoni, Alba, Souza, Márcia Maria de, Santos, Elisângela Trindade, Chan, Isaac S., Trindade, Guilherme Vaz de Melo, Choi, Steve S., Witek, Rafal P., Pereira, Fausto Edmundo Lima, Secor, William e, Andrade, Zilton de Araújo, Lambertucci, José Roberto, Diehl, Anna Mae
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/15120
Acceso en línea:https://arca.fiocruz.br/handle/icict/15120
Access Level:acceso abierto
Palabra clave:Cholangiocyte
Ductular proliferation
Osteopontin
Portal hypertension
Schistosomiasis mansoni
Symmers’ fibrosis
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dc.title.none.fl_str_mv Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
spellingShingle Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
Pereira, Thiago de Almeida
Cholangiocyte
Ductular proliferation
Osteopontin
Portal hypertension
Schistosomiasis mansoni
Symmers’ fibrosis
title_short Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_full Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_fullStr Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_full_unstemmed Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_sort Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
dc.creator.none.fl_str_mv Pereira, Thiago de Almeida
Syn, Wing-Kin
Machado, Mariana V.
Vidigal, Paula Vieira Teixeira
Resende, Vivian
Voieta, Izabela
Xie, Guanhua
Otoni, Alba
Souza, Márcia Maria de
Santos, Elisângela Trindade
Chan, Isaac S.
Trindade, Guilherme Vaz de Melo
Choi, Steve S.
Witek, Rafal P.
Pereira, Fausto Edmundo Lima
Secor, William e
Andrade, Zilton de Araújo
Lambertucci, José Roberto
Diehl, Anna Mae
author Pereira, Thiago de Almeida
author_facet Pereira, Thiago de Almeida
Syn, Wing-Kin
Machado, Mariana V.
Vidigal, Paula Vieira Teixeira
Resende, Vivian
Voieta, Izabela
Xie, Guanhua
Otoni, Alba
Souza, Márcia Maria de
Santos, Elisângela Trindade
Chan, Isaac S.
Trindade, Guilherme Vaz de Melo
Choi, Steve S.
Witek, Rafal P.
Pereira, Fausto Edmundo Lima
Secor, William e
Andrade, Zilton de Araújo
Lambertucci, José Roberto
Diehl, Anna Mae
author_role author
author2 Syn, Wing-Kin
Machado, Mariana V.
Vidigal, Paula Vieira Teixeira
Resende, Vivian
Voieta, Izabela
Xie, Guanhua
Otoni, Alba
Souza, Márcia Maria de
Santos, Elisângela Trindade
Chan, Isaac S.
Trindade, Guilherme Vaz de Melo
Choi, Steve S.
Witek, Rafal P.
Pereira, Fausto Edmundo Lima
Secor, William e
Andrade, Zilton de Araújo
Lambertucci, José Roberto
Diehl, Anna Mae
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.en.none.fl_str_mv Cholangiocyte
Ductular proliferation
Osteopontin
Portal hypertension
Schistosomiasis mansoni
Symmers’ fibrosis
topic Cholangiocyte
Ductular proliferation
Osteopontin
Portal hypertension
Schistosomiasis mansoni
Symmers’ fibrosis
description Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-07-25T19:35:54Z
dc.date.available.fl_str_mv 2016-07-25T19:35:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, T. A. et al. Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni. Clinical Science, v. 129, p. 875–883, 2015
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/15120
dc.identifier.issn.none.fl_str_mv 0143-5221
dc.identifier.doi.none.fl_str_mv 10.1042/CS20150117
identifier_str_mv PEREIRA, T. A. et al. Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni. Clinical Science, v. 129, p. 875–883, 2015
0143-5221
10.1042/CS20150117
url https://arca.fiocruz.br/handle/icict/15120
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2016-07-25T19:35:54Z2016-07-25T19:35:54Z2015PEREIRA, T. A. et al. Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni. Clinical Science, v. 129, p. 875–883, 20150143-5221https://arca.fiocruz.br/handle/icict/1512010.1042/CS20150117Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Duke University Medical Center. Department of Medicine. Division of Gastroenterology. Durham, U.S.A. / Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilFoundation for Liver Research. Institute of Hepatology. Liver Regeneration and Repair Research Group. London, U.K. / Loyola University. Department of Surgery. Chicago, Maywood, U.S.A.Duke University Medical Center. Department of Medicine. Division of Gastroenterology. Durham, U.S.A.Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrasilDuke University Medical Center. Department of Medicine. Division of Gastroenterology. Durham, U.S.A.Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilDuke University Medical Center. Department of Medicine. Division of Gastroenterology. Durham, U.S.A.Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrasilDuke University Medical Center. Department of Medicine. Division of Gastroenterology. Durham, U.S.A.Life Technologies. Frederick, MD, U.S.A.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, BrasilCenters for Disease Control and Prevention. Atlanta, GA, U.S.A.Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BAUniversidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrasilDuke University Medical Center. Department of Medicine. Division of Gastroenterology. Durham, U.S.A.Schistosomiasis is a major cause of portal hypertension worldwide. It associates with portal fibrosis that develops during chronic infection. The mechanisms by which the pathogen evokes these host responses remain unclear. We evaluated the hypothesis that schistosome eggs release factors that directly stimulate liver cells to produce osteopontin (OPN), a pro-fibrogenic protein that stimulates hepatic stellate cells to become myofibroblasts. We also investigated the utility of OPN as a biomarker of fibrosis and/or severity of portal hypertension. Cultured cholangiocytes, Kupffer cells and hepatic stellate cells were treated with soluble egg antigen (SEA); OPN production was quantified by quantitative reverse transcriptase polymerase chain reaction (qRTPCR) and ELISA; cell proliferation was assessed by BrdU (5-bromo-2'-deoxyuridine). Mice were infected with Schistosoma mansoni for 6 or 16 weeks to cause early or advanced fibrosis. Liver OPN was evaluated by qRTPCR and immunohistochemistry (IHC) and correlated with liver fibrosis and serum OPN. Livers from patients with schistosomiasis mansoni (early fibrosis n=15; advanced fibrosis n=72) or healthy adults (n=22) were immunostained for OPN and fibrosis markers. Results were correlated with plasma OPN levels and splenic vein pressures. SEA-induced cholangiocyte proliferation and OPN secretion (P<0.001 compared with controls). Cholangiocytes were OPN (+) in Schistosoma-infected mice and humans. Liver and serum OPN levels correlated with fibrosis stage (mice: r=0.861; human r=0.672, P=0.0001) and myofibroblast accumulation (mice: r=0.800; human: r=0.761, P=0.0001). Numbers of OPN (+) bile ductules strongly correlated with splenic vein pressure (r=0.778; P=0.001). S. mansoni egg antigens stimulate cholangiocyte proliferation and OPN secretion. OPN levels in liver and blood correlate with fibrosis stage and portal hypertension severity.engPortland PressSchistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCholangiocyteDuctular proliferationOsteopontinPortal hypertensionSchistosomiasis mansoniSymmers’ fibrosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZPereira, Thiago de AlmeidaSyn, Wing-KinMachado, Mariana V.Vidigal, Paula Vieira TeixeiraResende, VivianVoieta, IzabelaXie, GuanhuaOtoni, AlbaSouza, Márcia Maria deSantos, Elisângela TrindadeChan, Isaac S.Trindade, Guilherme Vaz de MeloChoi, Steve S.Witek, Rafal P.Pereira, Fausto Edmundo LimaSecor, William eAndrade, Zilton de AraújoLambertucci, José RobertoDiehl, Anna MaeLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/5c57c0ee-e65c-46dd-9529-6a14520b9057/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALPereira TA Schistosome-induced....pdfPereira TA Schistosome-induced....pdfapplication/pdf1592116https://arca.fiocruz.br/bitstreams/2c0614d1-0e1c-454e-8a39-cf1cf3a6a99e/download5dcd2e5f5e6a53407b91145c1607643eMD52trueAnonymousREADTEXTPereira TA Schistosome-induced....pdf.txtPereira TA Schistosome-induced....pdf.txtExtracted texttext/plain43412https://arca.fiocruz.br/bitstreams/40deecf4-2d15-40d3-8b9b-adce1712e99e/downloadac221fded81ebef3400a6e3a133407e3MD511falseAnonymousREADTHUMBNAILPereira TA Schistosome-induced....pdf.jpgPereira TA Schistosome-induced....pdf.jpgGenerated Thumbnailimage/jpeg43274https://arca.fiocruz.br/bitstreams/b489890e-3b74-4b43-bf9d-1801d6fc90a5/download4097ce63d061e5970365afc442c7e885MD512falseAnonymousREADicict/151202025-12-10 14:29:09.608open.accessoai:arca.fiocruz.br:icict/15120https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T17:29:09Repositório Institucional da FIOCRUZ (ARCA) - 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