Comparative analysis of Enterococcus faecalis biofilm formation on different substrates

INTRODUCTION: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. METHODS: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates...

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Detalles Bibliográficos
Autores: Guerreiro-Tanomaru, Juliane Maria, Faria Júnior, Norberto Batista de, Duarte, Marco Antonio Hungaro, Ordinola-Zapata, Ronald, Tanomaru-Filho, Mário
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Institución:Universidade Federal do Rio Grande do Norte (UFRN)
Repositorio:Repositório Institucional da UFRN
Idioma:inglés
OAI Identifier:oai:repositorio.ufrn.br:123456789/21750
Acceso en línea:https://repositorio.ufrn.br/jspui/handle/123456789/21750
Access Level:acceso abierto
Palabra clave:Biofilm
Confocal microscopy
Enterococcus faecalis
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spelling Comparative analysis of Enterococcus faecalis biofilm formation on different substratesBiofilmConfocal microscopyEnterococcus faecalisINTRODUCTION: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. METHODS: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37°C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (μm(3)), live bacteria biovolume (μm(3)), and substrate coverage (%) were quantified by using the BioImage_L software. Results obtained were analyzed by nonparametric tests (P = .05). RESULTS: Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P < .05) and hydroxyapatite the highest at 21 days (P < .05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P < .05). The percentages of coverage were similar among all substrates at 21 days (P > .05), but at 14 days, bovine bone presented the highest coverage (P < .05). CONCLUSIONS: E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated.2017-01-26T12:13:32Z2017-01-26T12:13:32Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGUERREIRO-TANOMARU, Juliane Maria et al. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. Journal of Endodontics , v. 39, n. 3, p. 346-350, 2013.https://repositorio.ufrn.br/jspui/handle/123456789/21750ark:/41046/001300000s0tqenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNGuerreiro-Tanomaru, Juliane MariaFaria Júnior, Norberto Batista deDuarte, Marco Antonio HungaroOrdinola-Zapata, RonaldTanomaru-Filho, Mário2021-12-21T17:12:52Zoai:repositorio.ufrn.br:123456789/21750Repositório InstitucionalPUBhttp://repositorio.ufrn.br/oai/repositorio@bczm.ufrn.bropendoar:2021-12-21T17:12:52Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.none.fl_str_mv Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
title Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
spellingShingle Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
Guerreiro-Tanomaru, Juliane Maria
Biofilm
Confocal microscopy
Enterococcus faecalis
title_short Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
title_full Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
title_fullStr Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
title_full_unstemmed Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
title_sort Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
dc.creator.none.fl_str_mv Guerreiro-Tanomaru, Juliane Maria
Faria Júnior, Norberto Batista de
Duarte, Marco Antonio Hungaro
Ordinola-Zapata, Ronald
Tanomaru-Filho, Mário
author Guerreiro-Tanomaru, Juliane Maria
author_facet Guerreiro-Tanomaru, Juliane Maria
Faria Júnior, Norberto Batista de
Duarte, Marco Antonio Hungaro
Ordinola-Zapata, Ronald
Tanomaru-Filho, Mário
author_role author
author2 Faria Júnior, Norberto Batista de
Duarte, Marco Antonio Hungaro
Ordinola-Zapata, Ronald
Tanomaru-Filho, Mário
author2_role author
author
author
author
dc.subject.por.fl_str_mv Biofilm
Confocal microscopy
Enterococcus faecalis
topic Biofilm
Confocal microscopy
Enterococcus faecalis
description INTRODUCTION: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. METHODS: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37°C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (μm(3)), live bacteria biovolume (μm(3)), and substrate coverage (%) were quantified by using the BioImage_L software. Results obtained were analyzed by nonparametric tests (P = .05). RESULTS: Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P < .05) and hydroxyapatite the highest at 21 days (P < .05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P < .05). The percentages of coverage were similar among all substrates at 21 days (P > .05), but at 14 days, bovine bone presented the highest coverage (P < .05). CONCLUSIONS: E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated.
publishDate 2013
dc.date.none.fl_str_mv 2013
2017-01-26T12:13:32Z
2017-01-26T12:13:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GUERREIRO-TANOMARU, Juliane Maria et al. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. Journal of Endodontics , v. 39, n. 3, p. 346-350, 2013.
https://repositorio.ufrn.br/jspui/handle/123456789/21750
dc.identifier.dark.fl_str_mv ark:/41046/001300000s0tq
identifier_str_mv GUERREIRO-TANOMARU, Juliane Maria et al. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. Journal of Endodontics , v. 39, n. 3, p. 346-350, 2013.
ark:/41046/001300000s0tq
url https://repositorio.ufrn.br/jspui/handle/123456789/21750
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv repositorio@bczm.ufrn.br
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