Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation

Regenerated cellulose scaffolds (RCS) may be used as alloplastic materials for tissue repair. In this work, the RCS were obtained by viscose process and characterized by scanning electron microscopy (SEM), wide angle X-ray diffraction (WAXD), Fourier transform infrared spectroscopy (FTIR) and thermo...

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Detalles Bibliográficos
Autores: De Araújo Júnior, Adalberto M. [UNESP], Braido, Guilherme [UNESP], Saska, Sybele [UNESP], Barud, Hernane S. [UNESP], Franchi, Leonardo P., Assunção, Rosana M.N., Scarel-Caminaga, Raquel M. [UNESP], Capote, Ticiana S.O. [UNESP], Messaddeq, Younès [UNESP], Ribeiro, Sidney J.L. [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/177536
Acceso en línea:http://dx.doi.org/10.1016/j.carbpol.2015.09.066
http://hdl.handle.net/11449/177536
Access Level:acceso abierto
Palabra clave:Cytotoxicity
Genotoxicity
Mutagenicity
Regenerated cellulose
Scaffold
Tissue repair
Descripción
Sumario:Regenerated cellulose scaffolds (RCS) may be used as alloplastic materials for tissue repair. In this work, the RCS were obtained by viscose process and characterized by scanning electron microscopy (SEM), wide angle X-ray diffraction (WAXD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetry analysis (TG). In vitro enzymatic degradation assay and toxicological assays were also evaluated. The physicochemical characterizations revealed the formation of a porous material with distinct thermal profile and crystallinity compared to pristine cellulose pulp. Enzymatic degradation assay revealed that lysozyme showed a mildest catalytic action when compared to cellulase, Tricoderma reesei (Tr). Nevertheless, both enzymes were efficient for degrading the RCS. RCS did not show cytotoxicity, mutagenic or genotoxic effects. The systematically characterization of this work suggests that RCS presented distinct features that make it a viable material for future studies related to the development of scaffolds for biological applications.

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