Diversity of surface structures and virulence genetic markers among enteroaggregative Escherichia coli (EAEC) strains with and without the EAEC DNA probe sequence

The expression of surface structures and the presence of DNA sequences related to putative virulence factors were investigated in 22 enteroaggregative Escherichia coli strains (EAEC). Fimbria was the most frequent (72.7%) structure identified. Only strains hybridising with the EAEC DNA probe carried...

Descripción completa

Detalles Bibliográficos
Autores: Suzart, Sergio [UNIFESP], Guth, Beatriz Ernestina Cabilio [UNIFESP], Pedroso, Margareth Z. [UNIFESP], Okafor, Udenma M. [UNIFESP], Gomes, Tania Aparecida Tardelli [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2001
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/26595
Acceso en línea:http://dx.doi.org/10.1111/j.1574-6968.2001.tb10751.x
http://repositorio.unifesp.br/handle/11600/26595
Access Level:acceso abierto
Palabra clave:enteroaggregative Escherichia coli
adherence
hemagglutination pattern
virulence marker
diarrhoea
Descripción
Sumario:The expression of surface structures and the presence of DNA sequences related to putative virulence factors were investigated in 22 enteroaggregative Escherichia coli strains (EAEC). Fimbria was the most frequent (72.7%) structure identified. Only strains hybridising with the EAEC DNA probe carried aggA, but one strain produced a similar but unrelated bundle-like structure. All probe-positive and 62.5% of the probe-negative strains carried the virulence genes tested; aspU and irp2 prevailed among the former strains. the EAEC probe-positive strains were more diverse, and some of these strains, which promoted cell detachment, also carried the hly and pap sequences, thus suggesting they might represent uropathogenic E. coli. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.