Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status

Previous studies demonstrate that the balance between pro- and anti-inflammatory mediators determines the stable or progressive nature of periapical granulomas by modulating the balance of the osteoclastogenic factor RANKL and its antagonist OPG. However, the cytokine networks operating in the devel...

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Autores: ARAUJO-PIRES, Ana Claudia, FRANCISCONI, Carolina Favaro, BIGUETTI, Claudia Cristina, CAVALLA, Franco, ARANHA, Andreza Maria Fabio, LETRA, Ariadne, TROMBONE, Ana Paula Favaro, FAVERI, Marcelo, SILVA, Renato Menezes, GARLET, Gustavo Pompermaier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Universidade de São Paulo (USP)
Repositorio:Journal of applied oral science (Online)
Idioma:inglés
OAI Identifier:oai:revistas.usp.br:article/84023
Acceso en línea:https://www.revistas.usp.br/jaos/article/view/84023
Access Level:acceso abierto
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spelling Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status Previous studies demonstrate that the balance between pro- and anti-inflammatory mediators determines the stable or progressive nature of periapical granulomas by modulating the balance of the osteoclastogenic factor RANKL and its antagonist OPG. However, the cytokine networks operating in the development of periapical lesions are quite more complex than what the simple pro- versus anti-inflammatory mediators' paradigm suggests. Here we simultaneously investigated the patterns of Th1, Th2, Th9, Th17, Th22, Thf, Tr1 and Tregs cytokines/markers expression in human periapical granulomas. Methods: The expression of TNF-α, IFN-γ, IL-17A, IL23, IL21, IL-33, IL-10, IL-4, IL-9, IL-22, FOXp3 markers (via RealTimePCR array) was accessed in active/progressive (N=40) versus inactive/stable (N=70) periapical granulomas (as determined by RANKL/OPG expression ratio), and also to compare these samples with a panel of control specimens (N=26). A cluster analysis of 13 cytokine levels was performed to examine possible clustering between the cytokines in a total of 110 granulomas. Results: The expression of all target cytokines was higher in the granulomas than in control samples. TNF-α, IFN-γ, IL-17A and IL-21 mRNA levels were significantly higher in active granulomas, while in inactive lesions the expression levels of IL-4, IL-9, IL-10, IL-22 and FOXp3 were higher than in active granulomas. Five clusters were identified in inactive lesion groups, being the variance in the expression levels of IL-17, IL-10, FOXp3, IFN-γ, IL-9, IL-33 and IL-4 statistically significant (KW pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/8402310.1590/1678-775720140140Journal of Applied Oral Science; Vol. 22 No. 4 (2014); 336-346Journal of Applied Oral Science; Vol. 22 Núm. 4 (2014); 336-346Journal of Applied Oral Science; v. 22 n. 4 (2014); 336-3461678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/84023/86869Copyright (c) 2014 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessARAUJO-PIRES, Ana Claudia FRANCISCONI, Carolina Favaro BIGUETTI, Claudia Cristina CAVALLA, Franco ARANHA, Andreza Maria Fabio LETRA, Ariadne TROMBONE, Ana Paula Favaro FAVERI, Marcelo SILVA, Renato Menezes GARLET, Gustavo Pompermaier 2014-08-26T23:56:55Zoai:revistas.usp.br:article/84023Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2014-08-26T23:56:55Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
title Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
spellingShingle Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
ARAUJO-PIRES, Ana Claudia
title_short Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
title_full Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
title_fullStr Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
title_full_unstemmed Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
title_sort Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status
dc.creator.none.fl_str_mv ARAUJO-PIRES, Ana Claudia
FRANCISCONI, Carolina Favaro
BIGUETTI, Claudia Cristina
CAVALLA, Franco
ARANHA, Andreza Maria Fabio
LETRA, Ariadne
TROMBONE, Ana Paula Favaro
FAVERI, Marcelo
SILVA, Renato Menezes
GARLET, Gustavo Pompermaier
author ARAUJO-PIRES, Ana Claudia
author_facet ARAUJO-PIRES, Ana Claudia
FRANCISCONI, Carolina Favaro
BIGUETTI, Claudia Cristina
CAVALLA, Franco
ARANHA, Andreza Maria Fabio
LETRA, Ariadne
TROMBONE, Ana Paula Favaro
FAVERI, Marcelo
SILVA, Renato Menezes
GARLET, Gustavo Pompermaier
author_role author
author2 FRANCISCONI, Carolina Favaro
BIGUETTI, Claudia Cristina
CAVALLA, Franco
ARANHA, Andreza Maria Fabio
LETRA, Ariadne
TROMBONE, Ana Paula Favaro
FAVERI, Marcelo
SILVA, Renato Menezes
GARLET, Gustavo Pompermaier
author2_role author
author
author
author
author
author
author
author
author
description Previous studies demonstrate that the balance between pro- and anti-inflammatory mediators determines the stable or progressive nature of periapical granulomas by modulating the balance of the osteoclastogenic factor RANKL and its antagonist OPG. However, the cytokine networks operating in the development of periapical lesions are quite more complex than what the simple pro- versus anti-inflammatory mediators' paradigm suggests. Here we simultaneously investigated the patterns of Th1, Th2, Th9, Th17, Th22, Thf, Tr1 and Tregs cytokines/markers expression in human periapical granulomas. Methods: The expression of TNF-α, IFN-γ, IL-17A, IL23, IL21, IL-33, IL-10, IL-4, IL-9, IL-22, FOXp3 markers (via RealTimePCR array) was accessed in active/progressive (N=40) versus inactive/stable (N=70) periapical granulomas (as determined by RANKL/OPG expression ratio), and also to compare these samples with a panel of control specimens (N=26). A cluster analysis of 13 cytokine levels was performed to examine possible clustering between the cytokines in a total of 110 granulomas. Results: The expression of all target cytokines was higher in the granulomas than in control samples. TNF-α, IFN-γ, IL-17A and IL-21 mRNA levels were significantly higher in active granulomas, while in inactive lesions the expression levels of IL-4, IL-9, IL-10, IL-22 and FOXp3 were higher than in active granulomas. Five clusters were identified in inactive lesion groups, being the variance in the expression levels of IL-17, IL-10, FOXp3, IFN-γ, IL-9, IL-33 and IL-4 statistically significant (KW p
publishDate 2014
dc.date.none.fl_str_mv 2014-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/84023
10.1590/1678-775720140140
url https://www.revistas.usp.br/jaos/article/view/84023
identifier_str_mv 10.1590/1678-775720140140
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/84023/86869
dc.rights.driver.fl_str_mv Copyright (c) 2014 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2014 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 22 No. 4 (2014); 336-346
Journal of Applied Oral Science; Vol. 22 Núm. 4 (2014); 336-346
Journal of Applied Oral Science; v. 22 n. 4 (2014); 336-346
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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