Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?

ProAKAP4 is a protein precursor of AKAP4 present in the principal piece of the flagellum, associated with sperm motility. It has been described in several species, but its concentration has never been measured in sex-sorted sperm. Thus, the study aimed to test the proAKAP4 as a suitable sperm qualit...

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Detalles Bibliográficos
Autores: de Almeida, Ana Beatriz Marques, Hidalgo, Myrian Megumy Tsunokawa, Trautwein, Luiz Guilherme Corsi, de Fátima Schnitzer, Josiana, Santos Silva, Letícia Amanda, de Moraes, Fábio Lucas Zito, Rizzoto, Guilherme [UNESP], Ferreira, João Carlos Pinheiro [UNESP], Martins, Maria Isabel Mello
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/299916
Acceso en línea:http://dx.doi.org/10.5433/1679-0359.2024v45n5p1413
https://hdl.handle.net/11449/299916
Access Level:acceso abierto
Palabra clave:AKAP4 protein
Non-sorted sperm
Sex-sorted sperm
X chromosome
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spelling Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?A proAKAP4 pode ser um biomarcador da qualidade de espermatozoides sexados-X de touros Nelore e Gir?AKAP4 proteinNon-sorted spermSex-sorted spermX chromosomeProAKAP4 is a protein precursor of AKAP4 present in the principal piece of the flagellum, associated with sperm motility. It has been described in several species, but its concentration has never been measured in sex-sorted sperm. Thus, the study aimed to test the proAKAP4 as a suitable sperm quality biomarker for Bos indicus X-sorted sperm. To achieve the study’s goal, 14 semen straws from different bulls were evaluated, six X-sorted and eight non-sorted. Sperm kinetics and morpho-functional evaluations were carried out, in addition to the concentration of proAKAP4. The characteristics evaluated were compared by the t-test and correlated by the Pearson’s correlation. A difference was found for total motility (P = 0.014), rapid sperm (P = 0.020), and sperm area (P = 0.013) between the non-sorted and X-sorted sperm. The proAKAP4 concentration was higher in the X-sorted sperm (X-sorted sperm: 67.54 ng/106 spermatozoa; non-sorted sperm: 29.76 ng/106 spermatozoa), but it was not correlated to the kinetics or morpho-functional characteristics evaluated. However, the proAKAP4 in non-sorted sperm showed a correlation to sperm total and progressive motility, rapid sperm, and sperm membrane integrity. The study concluded on the unreliability of the proAKAP4 as a biomarker to indicate sperm quality in the X-sorted sperm.Universidade Estadual de Londrina UEL, PRThe Animal Biotechnology Program Faculdade de Medicina Veterinária e Zootecnia UNESP, SPPostgraduate Program in Animal Biotechnology UNESP, SPPostgraduate Program in Animal Science UEL, PRThe Animal Biotechnology Program Faculdade de Medicina Veterinária e Zootecnia UNESP, SPPostgraduate Program in Animal Biotechnology UNESP, SPUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (UNESP)UEL2025-04-29T18:48:05Z2024-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1413-1422http://dx.doi.org/10.5433/1679-0359.2024v45n5p1413Semina:Ciencias Agrarias, v. 45, n. 5, p. 1413-1422, 2024.1679-03591676-546Xhttps://hdl.handle.net/11449/29991610.5433/1679-0359.2024v45n5p14132-s2.0-85203359420Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina:Ciencias Agrariasinfo:eu-repo/semantics/openAccessde Almeida, Ana Beatriz MarquesHidalgo, Myrian Megumy TsunokawaTrautwein, Luiz Guilherme Corside Fátima Schnitzer, JosianaSantos Silva, Letícia Amandade Moraes, Fábio Lucas ZitoRizzoto, Guilherme [UNESP]Ferreira, João Carlos Pinheiro [UNESP]Martins, Maria Isabel Mello2025-10-22T14:35:39Zoai:repositorio.unesp.br:11449/299916Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T14:35:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
A proAKAP4 pode ser um biomarcador da qualidade de espermatozoides sexados-X de touros Nelore e Gir?
title Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
spellingShingle Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
de Almeida, Ana Beatriz Marques
AKAP4 protein
Non-sorted sperm
Sex-sorted sperm
X chromosome
title_short Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
title_full Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
title_fullStr Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
title_full_unstemmed Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
title_sort Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
dc.creator.none.fl_str_mv de Almeida, Ana Beatriz Marques
Hidalgo, Myrian Megumy Tsunokawa
Trautwein, Luiz Guilherme Corsi
de Fátima Schnitzer, Josiana
Santos Silva, Letícia Amanda
de Moraes, Fábio Lucas Zito
Rizzoto, Guilherme [UNESP]
Ferreira, João Carlos Pinheiro [UNESP]
Martins, Maria Isabel Mello
author de Almeida, Ana Beatriz Marques
author_facet de Almeida, Ana Beatriz Marques
Hidalgo, Myrian Megumy Tsunokawa
Trautwein, Luiz Guilherme Corsi
de Fátima Schnitzer, Josiana
Santos Silva, Letícia Amanda
de Moraes, Fábio Lucas Zito
Rizzoto, Guilherme [UNESP]
Ferreira, João Carlos Pinheiro [UNESP]
Martins, Maria Isabel Mello
author_role author
author2 Hidalgo, Myrian Megumy Tsunokawa
Trautwein, Luiz Guilherme Corsi
de Fátima Schnitzer, Josiana
Santos Silva, Letícia Amanda
de Moraes, Fábio Lucas Zito
Rizzoto, Guilherme [UNESP]
Ferreira, João Carlos Pinheiro [UNESP]
Martins, Maria Isabel Mello
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Universidade Estadual Paulista (UNESP)
UEL
dc.subject.por.fl_str_mv AKAP4 protein
Non-sorted sperm
Sex-sorted sperm
X chromosome
topic AKAP4 protein
Non-sorted sperm
Sex-sorted sperm
X chromosome
description ProAKAP4 is a protein precursor of AKAP4 present in the principal piece of the flagellum, associated with sperm motility. It has been described in several species, but its concentration has never been measured in sex-sorted sperm. Thus, the study aimed to test the proAKAP4 as a suitable sperm quality biomarker for Bos indicus X-sorted sperm. To achieve the study’s goal, 14 semen straws from different bulls were evaluated, six X-sorted and eight non-sorted. Sperm kinetics and morpho-functional evaluations were carried out, in addition to the concentration of proAKAP4. The characteristics evaluated were compared by the t-test and correlated by the Pearson’s correlation. A difference was found for total motility (P = 0.014), rapid sperm (P = 0.020), and sperm area (P = 0.013) between the non-sorted and X-sorted sperm. The proAKAP4 concentration was higher in the X-sorted sperm (X-sorted sperm: 67.54 ng/106 spermatozoa; non-sorted sperm: 29.76 ng/106 spermatozoa), but it was not correlated to the kinetics or morpho-functional characteristics evaluated. However, the proAKAP4 in non-sorted sperm showed a correlation to sperm total and progressive motility, rapid sperm, and sperm membrane integrity. The study concluded on the unreliability of the proAKAP4 as a biomarker to indicate sperm quality in the X-sorted sperm.
publishDate 2024
dc.date.none.fl_str_mv 2024-09-01
2025-04-29T18:48:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5433/1679-0359.2024v45n5p1413
Semina:Ciencias Agrarias, v. 45, n. 5, p. 1413-1422, 2024.
1679-0359
1676-546X
https://hdl.handle.net/11449/299916
10.5433/1679-0359.2024v45n5p1413
2-s2.0-85203359420
url http://dx.doi.org/10.5433/1679-0359.2024v45n5p1413
https://hdl.handle.net/11449/299916
identifier_str_mv Semina:Ciencias Agrarias, v. 45, n. 5, p. 1413-1422, 2024.
1679-0359
1676-546X
10.5433/1679-0359.2024v45n5p1413
2-s2.0-85203359420
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Semina:Ciencias Agrarias
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1413-1422
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300724

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