Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil
The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine...
| Autores: | , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2013 |
| País: | Brasil |
| Recursos: | Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
| Repositório: | Brazilian Journal of Veterinary Research and Animal Science |
| Idioma: | inglês |
| OAI Identifier: | oai:revistas.usp.br:article/64604 |
| Acesso em linha: | https://www.revistas.usp.br/bjvras/article/view/64604 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Visceral Leishmaniasis Diagnosis ELISA IFAT PCR Leishmaniose Visceral Diagnóstico RIFI |
| Resumo: | The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florianópolis. |
|---|