Análise histomorfológica e molecular de lesões granulomatosas sugestivas de tuberculose bovina
Bovine tuberculosis is a chronic granulomatous infectious disease of zoonotic nature caused by Mycobacterium bovis. This bacterium belongs to the Mycobacterium tuberculosis complex which corresponds to a group of phylogenetically related mycobacteria, which have considerable difficulty in diagnosing...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.unesp.br:11449/121942 |
| Acceso en línea: | http://hdl.handle.net/11449/121942 |
| Access Level: | acceso abierto |
| Palabra clave: | Tuberculose em bovino Doenças transmissiveis - Diagnostico Reação em cadeia de polimerase Tecidos (Anatomia e fisiologia) - Analise Mycobacterium |
| Sumario: | Bovine tuberculosis is a chronic granulomatous infectious disease of zoonotic nature caused by Mycobacterium bovis. This bacterium belongs to the Mycobacterium tuberculosis complex which corresponds to a group of phylogenetically related mycobacteria, which have considerable difficulty in diagnosing directly in tissue samples. The objective of this study was to evaluate lymph nodes through histopathologic diagnosis and molecular targeting the direct detection of the agent in tissue. During the inspection line slaughter in a fridge, we selected 150 nodes with macroscopic lesions suggestive of tuberculosis. In histological analysis by staining Hematoxilia-eosin (HE), 100% of the samples had microscopic lesions compatible with tuberculosis. The coloring Zielh-Neelsen (ZN) revealed the presence of acid- resistant bacilli (AFB) in 6 (4%) samples. The extraction method for molecular analysis showed good results, but the amplification of DNA fragments of hsp65 genes and IS6110 resulted in low sensitivity. However, the reamplification of IS6110 gene resulted in 63.3% positivity, indicating the difficulty of tissue samples for molecular diagnosis related to this reduced amount of bacteria present in samples |
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