Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages

OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages - C6/36, VERO, BHK-21,...

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Autores: Ribeiro, Ana Cláudia da Silva, Carvalho, Carlos Alberto Marques de, Casseb, Samir Mansour Moraes, Rodrigues, Sueli Guerreiro, Vasconcelos, Pedro Fernando da Costa, Carvalho, Valéria Lima
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Institución:Instituto Evandro Chagas (IEC)
Repositorio:Revista Pan-Amazônica de Saúde (RPAS)
Idioma:inglés
OAI Identifier:oai:revista.iec.gov.br:article/304
Acceso en línea:https://ojs.iec.gov.br/rpas/article/view/304
Access Level:acceso abierto
Palabra clave:Arboviruses
Alphavirus
Mayaro virus
Chikungunya virus
Cell Lineage
Arbovírus
Vírus Mayaro
Vírus Chikungunya
Linhagem Celular
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oai_identifier_str oai:revista.iec.gov.br:article/304
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
Perfis de infecção do vírus Mayaro e do vírus Chikungunya em linhagens de células de mamíferos e mosquitos
title Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
spellingShingle Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
Ribeiro, Ana Cláudia da Silva
Arboviruses
Alphavirus
Mayaro virus
Chikungunya virus
Cell Lineage
Arbovírus
Alphavirus
Vírus Mayaro
Vírus Chikungunya
Linhagem Celular
title_short Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_full Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_fullStr Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_full_unstemmed Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_sort Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
dc.creator.none.fl_str_mv Ribeiro, Ana Cláudia da Silva
Carvalho, Carlos Alberto Marques de
Casseb, Samir Mansour Moraes
Rodrigues, Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
Carvalho, Valéria Lima
author Ribeiro, Ana Cláudia da Silva
author_facet Ribeiro, Ana Cláudia da Silva
Carvalho, Carlos Alberto Marques de
Casseb, Samir Mansour Moraes
Rodrigues, Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
Carvalho, Valéria Lima
author_role author
author2 Carvalho, Carlos Alberto Marques de
Casseb, Samir Mansour Moraes
Rodrigues, Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
Carvalho, Valéria Lima
author2_role author
author
author
author
author
dc.subject.por.fl_str_mv Arboviruses
Alphavirus
Mayaro virus
Chikungunya virus
Cell Lineage
Arbovírus
Alphavirus
Vírus Mayaro
Vírus Chikungunya
Linhagem Celular
topic Arboviruses
Alphavirus
Mayaro virus
Chikungunya virus
Cell Lineage
Arbovírus
Alphavirus
Vírus Mayaro
Vírus Chikungunya
Linhagem Celular
description OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages - C6/36, VERO, BHK-21, and LLC-MK2 - under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.iec.gov.br/rpas/article/view/304
10.5123/S2176-62232018000400003
url https://ojs.iec.gov.br/rpas/article/view/304
identifier_str_mv 10.5123/S2176-62232018000400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.iec.gov.br/rpas/article/view/304/157
https://ojs.iec.gov.br/rpas/article/view/304/158
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/xml
application/pdf
dc.publisher.none.fl_str_mv Instituto Evandro Chagas/SCTIE/MS
publisher.none.fl_str_mv Instituto Evandro Chagas/SCTIE/MS
dc.source.none.fl_str_mv Revista Pan-Amazônica de Saúde; v. 9 n. 4 (2018); 11
Pan-Amazonian Journal of Health; Vol. 9 No. 4 (2018); 11
Revista Pan-Amazônica de Saúde; Vol. 9 Núm. 4 (2018); 11
2176-6223
2176-6215
reponame:Revista Pan-Amazônica de Saúde (RPAS)
instname:Instituto Evandro Chagas (IEC)
instacron:IEC
instname_str Instituto Evandro Chagas (IEC)
instacron_str IEC
institution IEC
reponame_str Revista Pan-Amazônica de Saúde (RPAS)
collection Revista Pan-Amazônica de Saúde (RPAS)
repository.name.fl_str_mv Revista Pan-Amazônica de Saúde (RPAS) - Instituto Evandro Chagas (IEC)
repository.mail.fl_str_mv revista@iec.gov.br
_version_ 1853661774664957952
spelling Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineagesPerfis de infecção do vírus Mayaro e do vírus Chikungunya em linhagens de células de mamíferos e mosquitosArbovirusesAlphavirusMayaro virusChikungunya virusCell LineageArbovírusAlphavirusVírus MayaroVírus ChikungunyaLinhagem Celular OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages - C6/36, VERO, BHK-21, and LLC-MK2 - under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples. OBJETIVO: Traçar o perfil infeccioso do vírus Mayaro (MAYV) e do vírus Chikungunya (CHIKV) em quatro diferentes linhagens de células. MATERIAIS E MÉTODOS: Os isolados desses vírus foram utilizados para infectar culturas das seguintes linhagens celulares - C6/36, VERO, BHK-21 e LLC-MK2 - sob a multiplicidade de infecção de 1 PFU/célula, as quais foram monitoradas por até 96 h pós-infecção (hpi) por microscopia de contraste de fase para registrar o efeito citopático (CPE); e, em seguida, foram submetidos a ensaios de imunofluorescência indireta (iIF) e reação em cadeia da polimerase de transcrição reversa quantitativa (qRT-PCR) para detecção de antígenos virais e determinação da carga de RNA, respectivamente. RESULTADOS: Para ambos os vírus, as células VERO e BHK-21 apresentaram CPE de 24 hpi, as LLC-MK2 apresentaram CPE a partir de 48 hpi e as C6/36 não apresentaram qualquer CPE. Nos ensaios de iIF, todas as células já se tornaram positivas a 6 hpi, com exceção das células LLC-MK2 e BHK-21 infectadas com CHIKV. Finalmente, os ensaios qRT-PCR mostraram que, em geral, as células infectadas com MAYV apresentaram aumento da carga de RNA, embora as células LLC-MK2 infectadas pelo CHIKV tenham apresentado a maior carga de RNA. CONCLUSÃO: Ao avaliar o comportamento desses arbovírus em diferentes tipos de culturas de células, incluindo alguns não comumente utilizados na rotina do diagnóstico laboratorial, o presente estudo fornece não apenas uma melhor compreensão da cinética de replicação de MAYV e CHIKV, mas também oportunidades para a otimização de seu diagnóstico em amostras biológicas. Instituto Evandro Chagas/SCTIE/MS2019-06-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/xmlapplication/pdfhttps://ojs.iec.gov.br/rpas/article/view/30410.5123/S2176-62232018000400003Revista Pan-Amazônica de Saúde; v. 9 n. 4 (2018); 11Pan-Amazonian Journal of Health; Vol. 9 No. 4 (2018); 11Revista Pan-Amazônica de Saúde; Vol. 9 Núm. 4 (2018); 112176-62232176-6215reponame:Revista Pan-Amazônica de Saúde (RPAS)instname:Instituto Evandro Chagas (IEC)instacron:IECenghttps://ojs.iec.gov.br/rpas/article/view/304/157https://ojs.iec.gov.br/rpas/article/view/304/158info:eu-repo/semantics/openAccessRibeiro, Ana Cláudia da SilvaCarvalho, Carlos Alberto Marques deCasseb, Samir Mansour MoraesRodrigues, Sueli GuerreiroVasconcelos, Pedro Fernando da CostaCarvalho, Valéria Lima2021-01-01T17:56:24Zoai:revista.iec.gov.br:article/304Revistahttp://revista.iec.gov.br/PRIhttps://ojs.iec.gov.br/index.php/rpas/oairevista@iec.gov.br2176-62152176-6223opendoar:2021-01-01T17:56:24Revista Pan-Amazônica de Saúde (RPAS) - Instituto Evandro Chagas (IEC)false
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