Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant
Lemna aequinoctialis (duckweed) is the smallest and fast-growing aquatic plant species producing protein-rich biomass with high protein nutritional value, phytoremediation capacity, and nutrient removal from wastewater. Duckweed may also be used as a new potential bioreactor for biological products,...
| Autores: | , , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/298965 |
| Acesso em linha: | http://dx.doi.org/10.1080/15287394.2024.2397643 https://hdl.handle.net/11449/298965 |
| Access Level: | acceso abierto |
| Palavra-chave: | biomass cell culture cytotoxicity duckweed extracts IMTA Lemna aequinoctialis MTT nutritional composition |
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| dc.title.none.fl_str_mv |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant |
| title |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant |
| spellingShingle |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant Nati, Natalia biomass cell culture cytotoxicity duckweed extracts IMTA Lemna aequinoctialis MTT nutritional composition |
| title_short |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant |
| title_full |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant |
| title_fullStr |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant |
| title_full_unstemmed |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant |
| title_sort |
Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plant |
| dc.creator.none.fl_str_mv |
Nati, Natalia Galter, Iasmini Nicoli Souza Costa, Iara Fabre Garcia, Emily Amorim Lopes, Gabriel Seibert França, Hildegardo Pompermayer Machado, Levi [UNESP] da Silva, Regildo Márcio Gonçalves [UNESP] Tamie Matsumoto, Silvia |
| author |
Nati, Natalia |
| author_facet |
Nati, Natalia Galter, Iasmini Nicoli Souza Costa, Iara Fabre Garcia, Emily Amorim Lopes, Gabriel Seibert França, Hildegardo Pompermayer Machado, Levi [UNESP] da Silva, Regildo Márcio Gonçalves [UNESP] Tamie Matsumoto, Silvia |
| author_role |
author |
| author2 |
Galter, Iasmini Nicoli Souza Costa, Iara Fabre Garcia, Emily Amorim Lopes, Gabriel Seibert França, Hildegardo Pompermayer Machado, Levi [UNESP] da Silva, Regildo Márcio Gonçalves [UNESP] Tamie Matsumoto, Silvia |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Federal University of Espírito Santo (UFES) Universidade Federal de São Carlos (UFSCar) Federal Institute of Espírito Santo (IFES) Universidade Estadual Paulista (UNESP) |
| dc.subject.por.fl_str_mv |
biomass cell culture cytotoxicity duckweed extracts IMTA Lemna aequinoctialis MTT nutritional composition |
| topic |
biomass cell culture cytotoxicity duckweed extracts IMTA Lemna aequinoctialis MTT nutritional composition |
| description |
Lemna aequinoctialis (duckweed) is the smallest and fast-growing aquatic plant species producing protein-rich biomass with high protein nutritional value, phytoremediation capacity, and nutrient removal from wastewater. Duckweed may also be used as a new potential bioreactor for biological products, such as vaccines, antibodies, and pharmaceutical proteins. Based upon the potential importanc of L. aequinoctialis in phytoremediation and as a bioreactor the aim of this study was to (1) characterize the chemical and nutritional profiles of L. aequinoctialis biomass utilizing an integrated multi-trophic aquaculture system (IMTA) and a pond, and (2) investigate the cytotoxic potential of different concentrations of organic extracts and fractions using the MTT bioassay. EDXRF and ICP-MS analyses indicated the presence of trace elements in lower amounts in relation to the biomass of L. aequinoctialis in the lagoon, emphasizing the importance of plant inclusion management to reduce bioaccumulation of these elements. Analysis of mineral profiles, fatty acids, and amino acids indicated a satisfactory nutritional composition for the use of biomass as a bioproduct. Pigment analysis showed a high concentration of carotenoids, especially astaxanthin. After standardizing the controls, the MTT cell viability test was carried out utilizing rat hepatoma cell line (HTC), which are metabolizing cells that were treated with aqueous or ethanolic extracts and the dichloromethane, ethyl acetate, and methanol fractions at different concentrations. No apparent cytotoxic potential was observed following treatments, since there was no significant reduction in cell viability. Therefore, this study provides information regarding the biomass of L. aequinoctialis derived from the IMTA system, which might support further research into the application of this species as a bioproduct. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-01-01 2025-04-29T18:41:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://dx.doi.org/10.1080/15287394.2024.2397643 Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 87, n. 23, p. 934-952, 2024. 1087-2620 1528-7394 https://hdl.handle.net/11449/298965 10.1080/15287394.2024.2397643 2-s2.0-85203335860 |
| url |
http://dx.doi.org/10.1080/15287394.2024.2397643 https://hdl.handle.net/11449/298965 |
| identifier_str_mv |
Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 87, n. 23, p. 934-952, 2024. 1087-2620 1528-7394 10.1080/15287394.2024.2397643 2-s2.0-85203335860 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Journal of Toxicology and Environmental Health - Part A: Current Issues |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
934-952 |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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1853671434049552384 |
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Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the Lemna aequinoctialis (duckweed) aquatic plantbiomasscell culturecytotoxicityduckweedextractsIMTALemna aequinoctialisMTTnutritional compositionLemna aequinoctialis (duckweed) is the smallest and fast-growing aquatic plant species producing protein-rich biomass with high protein nutritional value, phytoremediation capacity, and nutrient removal from wastewater. Duckweed may also be used as a new potential bioreactor for biological products, such as vaccines, antibodies, and pharmaceutical proteins. Based upon the potential importanc of L. aequinoctialis in phytoremediation and as a bioreactor the aim of this study was to (1) characterize the chemical and nutritional profiles of L. aequinoctialis biomass utilizing an integrated multi-trophic aquaculture system (IMTA) and a pond, and (2) investigate the cytotoxic potential of different concentrations of organic extracts and fractions using the MTT bioassay. EDXRF and ICP-MS analyses indicated the presence of trace elements in lower amounts in relation to the biomass of L. aequinoctialis in the lagoon, emphasizing the importance of plant inclusion management to reduce bioaccumulation of these elements. Analysis of mineral profiles, fatty acids, and amino acids indicated a satisfactory nutritional composition for the use of biomass as a bioproduct. Pigment analysis showed a high concentration of carotenoids, especially astaxanthin. After standardizing the controls, the MTT cell viability test was carried out utilizing rat hepatoma cell line (HTC), which are metabolizing cells that were treated with aqueous or ethanolic extracts and the dichloromethane, ethyl acetate, and methanol fractions at different concentrations. No apparent cytotoxic potential was observed following treatments, since there was no significant reduction in cell viability. Therefore, this study provides information regarding the biomass of L. aequinoctialis derived from the IMTA system, which might support further research into the application of this species as a bioproduct.Center for Human and Natural Sciences Department of Biological Sciences Federal University of Espírito Santo (UFES), Espírito SantoDepartment of Physiological Sciences Federal University of São Carlos (DCF/UFSCar)Federal Institute of Espírito Santo (IFES), Espírito SantoSchool of Agricultural Sciences Department of Fisheries Engineering São Paulo State University (UNESP)School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloSchool of Sciences Humanities and Languages Department of Biotechnology São Paulo State University (UNESP), São PauloInstitute of Chemistry São Paulo State University (UNESP), São PauloSchool of Agricultural Sciences Department of Fisheries Engineering São Paulo State University (UNESP)School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloSchool of Sciences Humanities and Languages Department of Biotechnology São Paulo State University (UNESP), São PauloInstitute of Chemistry São Paulo State University (UNESP), São PauloFederal University of Espírito Santo (UFES)Universidade Federal de São Carlos (UFSCar)Federal Institute of Espírito Santo (IFES)Universidade Estadual Paulista (UNESP)2025-04-29T18:41:01Z2024-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article934-952http://dx.doi.org/10.1080/15287394.2024.2397643Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 87, n. 23, p. 934-952, 2024.1087-26201528-7394https://hdl.handle.net/11449/29896510.1080/15287394.2024.23976432-s2.0-85203335860Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Toxicology and Environmental Health - Part A: Current Issuesinfo:eu-repo/semantics/openAccessNati, NataliaGalter, Iasmini NicoliSouza Costa, IaraFabre Garcia, EmilyAmorim Lopes, GabrielSeibert França, HildegardoPompermayer Machado, Levi [UNESP]da Silva, Regildo Márcio Gonçalves [UNESP]Tamie Matsumoto, Silvia2025-05-28T08:53:13Zoai:repositorio.unesp.br:11449/298965Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-05-28T08:53:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
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15,301603 |