Reduction in the inflammatory markers CD4, IL-1, IL-6 and TNFα in dogs with keratoconjunctivitis sicca treated topically with mesenchymal stem cells

Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellent model for understanding this disease, as the origin of KCS in dogs is like that in humans. The objective of this study was to localize and quantify immunological markers, such as CD4 lymphocytes, inter...

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Detalles Bibliográficos
Autores: Sgrignoli, Marcos Rogério, Silva, Danielle Alves, Nascimento, Felipe Franco, Sgrignoli, Danielle Antonelli Motta, Nai, Gisele Alborghetti, da Silva, Márcia Guimarães [UNESP], de Barros, Michele Andrade, Bittencourt, Maura Krähembühl Wanderley, de Morais, Bruna Pereira, Dinallo, Heloíse Rangel, Foglia, Bruna Toledo Duran, Cabrera, Wellington Bott, Fares, Elaine Carrion, Andrade, Silvia Franco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/187961
Acceso en línea:http://dx.doi.org/10.1016/j.scr.2019.101525
http://hdl.handle.net/11449/187961
Access Level:acceso abierto
Palabra clave:Dogs
Dry eye
Immunological markers
Keratoconjunctivitis sicca
Mesenchymal stem cells
Topical
Descripción
Sumario:Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellent model for understanding this disease, as the origin of KCS in dogs is like that in humans. The objective of this study was to localize and quantify immunological markers, such as CD4 lymphocytes, interleukin (IL)-1, IL-6 and tumor necrosis factor alpha (TNFα), before and after topical treatment with mesenchymal stem cells (MSCs). Twenty-two dogs positive for KCS were topically treated with 50 μL (1 × 106 MSCs) in the conjunctival sac and were evaluated for 6 months. The levels of the markers CD4, IL-6, IL-1 and TNFα were analyzed in conjunctival biopsy and cytology of the third eyelid gland by immunohistochemistry and immunocytochemistry. The results showed that before treatment, there was marked expression of all the markers (CD4, IL-6, IL-1 and TNFα), and after 6 months, there were significant (p < .05) reductions in the expression levels of all the markers. These results demonstrated that topical MSC treatment promotes a significant decrease in the expression levels of these inflammatory markers and could be used as adjuvant therapy in the treatment of KCS in dogs and humans. In addition, these markers can be excellent tools for diagnosing and analyzing the progression of KCS.

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