Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse

Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET3...

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Detalhes bibliográficos
Autores: Kuniyoshi, Taís Mayumi, Mendonça, Carlos Miguel Nóbrega, Vieira, Viviane Borges, Robl, Diogo, de Melo Franco, Bernadette Dora Gombossy, Todorov, Svetoslav Dimitrov, Tomé, Elisabetta, O'Connor, Paula Mary, Converti, Attilio, Araújo, Welington Luiz, Vasconcellos, Lívia Paula Silva Palmeiras, Varani, Alessandro de Mello [UNESP], Cotter, Paul David, Rabelo, Sarita Cândida [UNESP], Oliveira, Ricardo Pinheiro de Souza
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositório:Repositório Institucional da UNESP
Idioma:inglês
OAI Identifier:oai:repositorio.unesp.br:11449/229199
Acesso em linha:http://dx.doi.org/10.1016/j.biortech.2021.125565
http://hdl.handle.net/11449/229199
Access Level:Acceso aberto
Palavra-chave:Bacteriocin
Hemicellulose hydrolysate
Pediococcus pentosaceus
Sugarcane bagasse
Descrição
Resumo:Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.

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