Probióticos encapsulados aplicados em produto cárneo

The aim of this study was to encapsulate probiotic microorganisms, evaluating their resistance against harsh environments and the use in dry fermented sausages. L. plantarum ATCC 8041 and E. faecium ATCC 700221 were encapsulate in alginate beads in association with inulin, milk powder and trehalose...

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Bibliographic Details
Author: Cavalheiro, Carlos Pasqualin
Format: doctoral thesis
Status:Published version
Publication Date:2016
Country:Brasil
Institution:Universidade Federal de Santa Maria (UFSM)
Repository:Manancial - Repositório Digital da UFSM
Language:Portuguese
OAI Identifier:oai:repositorio.ufsm.br:1/3414
Online Access:http://repositorio.ufsm.br/handle/1/3414
Access Level:Open access
Keyword:Probióticos
Encapsulação
Embutido fermentado
Lactobacillus plantarum
Emulsões duplas
Probiotics
Encapsulation
Dry fermented sausage
Double emulsions
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
Description
Summary:The aim of this study was to encapsulate probiotic microorganisms, evaluating their resistance against harsh environments and the use in dry fermented sausages. L. plantarum ATCC 8041 and E. faecium ATCC 700221 were encapsulate in alginate beads in association with inulin, milk powder and trehalose through extrusion technique and submitted to stressful environments such as high temperatures, high concentrations of sodium chloride and sodium nitrite and refrigerated storage period. Subsequently, L. plantarum was added to dry fermented sausages as free cells or encapsulated using extrusion, emulsion and double emulsion techniques. Encapsulation was effective to protect probiotics against stress treatments, mainly 70 ºC temperature and high concentrations of sodium chloride and sodium nitrite. Furthermore, encapsulated probiotics kept their viability throughout 70 days of refrigerated storage. In addition, the use of encapsulated L. plantarum did not change physico-chemical properties of dry fermented sausage during processing and storage. However, the extrusion technique seems to be more effective to keep higher counts of L. plantarum in dry fermented sausages during processing and storage. The addition of L. plantarum encapsulated in emulsion and double emulsion increased lipid oxidation in dry fermented sausages. Furtermore, in sensory analysis, dry fermented sausages containing L. plantarum encapsulated in emulsion had lower scores than control treatment in flavor, hardness, texture and overall acceptance attributes.