Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
Purpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was e...
| Autores: | , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2010 |
| País: | Brasil |
| Recursos: | Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
| Repositório: | Revista odonto ciência (Online) |
| Idioma: | inglês |
| OAI Identifier: | oai:ojs.revistaseletronicas.pucrs.br:article/5043 |
| Acesso em linha: | https://revistaseletronicas.pucrs.br/fo/article/view/5043 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Human identification Forensic Dentistry Forensic Medicine DNA molecular biology saliva Identificação Humana |
| Resumo: | Purpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA. |
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