Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.

Background: In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less complicated clinical and laborat...

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Autores: Lima, Ana Carolina de Oliveira de, Schmid, Karen Barros, Melo, Hilda Ferreira de, Athayde, Rafaella Christine, Monte, Rossiclea Lins, Almeida, Isabela Neves de, Miranda, Silvana Spíndola de, Kritski, Afranio Lineu, Rossetti, Maria Lucia Rosa, Santos, Marcelo Cordeiro dos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Brasil
Recursos:Universidade Federal de Ouro Preto (UFOP)
Repositorio:Repositório Institucional da UFOP
Idioma:inglés
OAI Identifier:oai:repositorio.ufop.br:123456789/17573
Acesso em linha:http://www.repositorio.ufop.br/jspui/handle/123456789/17573
https://doi.org/10.1590/0037-8682-0613-2021
Access Level:acceso abierto
Palavra-chave:HIV infections
Molecular diagnostic technique
Nontuberculous mycobacteria
Prevalence
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spelling Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.HIV infectionsMolecular diagnostic techniqueNontuberculous mycobacteriaPrevalenceBackground: In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less complicated clinical and laboratory techniques. Methods: In this retrospective study, NTM species isolated from pulmonary, extrapulmonary, and disseminated samples from patients treated at a TB/HIV reference unit in the State of Amazonas from 2011 to 2014 were identified through a combination of molecular techniques. Results: To identify the molecular technique, 50 cryopreserved NTM cultures were recovered and subcultivated in culture medium. The potentially pathogenic NTM species identified were M. avium, M. intracellulare, M. kansasii, M. chelonae, M. abscessus, M. fortuitum, and M. peregrinum. Results of GenoType® showed moderate agreement with those of genomic sequencing (kappa = 0.60), whereas the results obtained by the PRA-hsp65 technique disagreed with the results obtained by sequencing (kappa = 0.49). Conclusions: Our findings highlight that GenoType CM is a good method for the identification of NTM, as well as the need for the application of standardized criteria, such as those set forth by the ATS.2023-10-16T20:44:35Z2023-10-16T20:44:35Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLIMA, A. C. de O. de et al. Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil. Sociedade Brasileira de Medicina Tropical, v. 55, artigo e0613-2021, 2022. Disponível em: <https://www.scielo.br/j/rsbmt/a/WHpMgr9PXzxNwDDzT735sqJ/?lang=en>. Acesso em: 01 ago. 2023.1678-9849http://www.repositorio.ufop.br/jspui/handle/123456789/17573https://doi.org/10.1590/0037-8682-0613-2021ark:/61566/0013000000z64This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLima, Ana Carolina de Oliveira deSchmid, Karen BarrosMelo, Hilda Ferreira deAthayde, Rafaella ChristineMonte, Rossiclea LinsAlmeida, Isabela Neves deMiranda, Silvana Spíndola deKritski, Afranio LineuRossetti, Maria Lucia RosaSantos, Marcelo Cordeiro dos2025-08-27T21:13:49Zoai:repositorio.ufop.br:123456789/17573Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332025-08-27T21:13:49Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
title Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
spellingShingle Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
Lima, Ana Carolina de Oliveira de
HIV infections
Molecular diagnostic technique
Nontuberculous mycobacteria
Prevalence
title_short Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
title_full Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
title_fullStr Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
title_full_unstemmed Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
title_sort Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
dc.creator.none.fl_str_mv Lima, Ana Carolina de Oliveira de
Schmid, Karen Barros
Melo, Hilda Ferreira de
Athayde, Rafaella Christine
Monte, Rossiclea Lins
Almeida, Isabela Neves de
Miranda, Silvana Spíndola de
Kritski, Afranio Lineu
Rossetti, Maria Lucia Rosa
Santos, Marcelo Cordeiro dos
author Lima, Ana Carolina de Oliveira de
author_facet Lima, Ana Carolina de Oliveira de
Schmid, Karen Barros
Melo, Hilda Ferreira de
Athayde, Rafaella Christine
Monte, Rossiclea Lins
Almeida, Isabela Neves de
Miranda, Silvana Spíndola de
Kritski, Afranio Lineu
Rossetti, Maria Lucia Rosa
Santos, Marcelo Cordeiro dos
author_role author
author2 Schmid, Karen Barros
Melo, Hilda Ferreira de
Athayde, Rafaella Christine
Monte, Rossiclea Lins
Almeida, Isabela Neves de
Miranda, Silvana Spíndola de
Kritski, Afranio Lineu
Rossetti, Maria Lucia Rosa
Santos, Marcelo Cordeiro dos
author2_role author
author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv HIV infections
Molecular diagnostic technique
Nontuberculous mycobacteria
Prevalence
topic HIV infections
Molecular diagnostic technique
Nontuberculous mycobacteria
Prevalence
description Background: In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less complicated clinical and laboratory techniques. Methods: In this retrospective study, NTM species isolated from pulmonary, extrapulmonary, and disseminated samples from patients treated at a TB/HIV reference unit in the State of Amazonas from 2011 to 2014 were identified through a combination of molecular techniques. Results: To identify the molecular technique, 50 cryopreserved NTM cultures were recovered and subcultivated in culture medium. The potentially pathogenic NTM species identified were M. avium, M. intracellulare, M. kansasii, M. chelonae, M. abscessus, M. fortuitum, and M. peregrinum. Results of GenoType® showed moderate agreement with those of genomic sequencing (kappa = 0.60), whereas the results obtained by the PRA-hsp65 technique disagreed with the results obtained by sequencing (kappa = 0.49). Conclusions: Our findings highlight that GenoType CM is a good method for the identification of NTM, as well as the need for the application of standardized criteria, such as those set forth by the ATS.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023-10-16T20:44:35Z
2023-10-16T20:44:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LIMA, A. C. de O. de et al. Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil. Sociedade Brasileira de Medicina Tropical, v. 55, artigo e0613-2021, 2022. Disponível em: <https://www.scielo.br/j/rsbmt/a/WHpMgr9PXzxNwDDzT735sqJ/?lang=en>. Acesso em: 01 ago. 2023.
1678-9849
http://www.repositorio.ufop.br/jspui/handle/123456789/17573
https://doi.org/10.1590/0037-8682-0613-2021
dc.identifier.dark.fl_str_mv ark:/61566/0013000000z64
identifier_str_mv LIMA, A. C. de O. de et al. Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil. Sociedade Brasileira de Medicina Tropical, v. 55, artigo e0613-2021, 2022. Disponível em: <https://www.scielo.br/j/rsbmt/a/WHpMgr9PXzxNwDDzT735sqJ/?lang=en>. Acesso em: 01 ago. 2023.
1678-9849
ark:/61566/0013000000z64
url http://www.repositorio.ufop.br/jspui/handle/123456789/17573
https://doi.org/10.1590/0037-8682-0613-2021
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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