Decalcification dynamic of dog mineralized tissue by microwaves

The aim of this article is to present the decalcification process dynamic of mineralized tissue in dogs, teeth and jaw, comparing the traditional decalcification method, immersion, and microwave, immersion followed by irradiation using a domestic microwave oven, accompanying the liberation of calciu...

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Detalles Bibliográficos
Autores: Pitol, Dimitrius Leonardo [UNESP], Issa, Joáo Paulo Mardegan, Caetano, Flávio Henrique [UNESP], Lunardi, Laurelúcia Orive
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/69696
Acceso en línea:http://dx.doi.org/10.4067/S0717-95022007000200011
http://hdl.handle.net/11449/69696
Access Level:acceso abierto
Palabra clave:Decalcification
Dog
Microwaves
Mineralized tissue
Solution
Descripción
Sumario:The aim of this article is to present the decalcification process dynamic of mineralized tissue in dogs, teeth and jaw, comparing the traditional decalcification method, immersion, and microwave, immersion followed by irradiation using a domestic microwave oven, accompanying the liberation of calcium through spectrophotometer of atomic absorption. It was used as decalcified agent, EDTA solution or nitric acid. The results showed that with the use of nitric acid (5%), after 15 days, the irradiated fragments could be processed for histological analysis, otherwise the tooth not irradiated need to be submerged for 65 days. The EDTA decalcified action was slower than the nitric acid. The histological observations of the irradiated samples showed an excellent preservation of the morphological characteristics, independently of the decalcified agent used. © 2007 Sociedad Chilena de Anatomía.