Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells

Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar pr...

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Autores: Silva, Neusa Pereira da [UNIFESP], Christofolini, Denise Maria [UNIFESP], Mortara, Renato Arruda [UNIFESP], Andrade, Luiz Eduardo Coelho [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/2156
Acceso en línea:http://dx.doi.org/10.1590/S0100-879X2004000700008
http://repositorio.unifesp.br/handle/11600/2156
Access Level:acceso abierto
Palabra clave:Cajal body
Coiled body
Micronuclei
Cell nucleolus
Nucleolus organizer region
RNA polymerase I
Nucleolar proteins
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spelling Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cellsCajal bodyCoiled bodyMicronucleiCell nucleolusNucleolus organizer regionRNA polymerase INucleolar proteinsCajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or two micronuclei containing nucleolus. By confocal microscopy we showed that in most micronuclei lacking a typical nucleolus a variable number of round structures were stained by antibodies against fibrillarin, NOR-90/hUBF protein, and coilin. These bodies were regarded as CB-like structures and were not stained by anti-PM/Scl and anti-To/Th antibodies. Anti-RNA polymerase I antibodies also reacted with CB-like structures in some micronuclei lacking nucleolus. The demonstration that a set of proteins involved in RNA/RNP biogenesis, namely coilin, fibrillarin, NOR-90/hUBF, and RNA polymerase I gather in CB-like structures present in nucleoli-devoid micronuclei may contribute to shed some light into the understanding of CB function.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MedicinaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, EPM, Depto. de MedicinaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:31:12Z2015-06-14T13:31:12Z2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion997-1003application/pdfhttp://dx.doi.org/10.1590/S0100-879X2004000700008Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 997-1003, 2004.10.1590/S0100-879X2004000700008S0100-879X2004000700008.pdf0100-879XS0100-879X2004000700008http://repositorio.unifesp.br/handle/11600/2156WOS:000222450200008ark:/48912/0013000025qccengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPSilva, Neusa Pereira da [UNIFESP]Christofolini, Denise Maria [UNIFESP]Mortara, Renato Arruda [UNIFESP]Andrade, Luiz Eduardo Coelho [UNIFESP]2024-07-29T17:24:59Zoai:repositorio.unifesp.br:11600/2156Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T17:24:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
title Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
spellingShingle Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
Silva, Neusa Pereira da [UNIFESP]
Cajal body
Coiled body
Micronuclei
Cell nucleolus
Nucleolus organizer region
RNA polymerase I
Nucleolar proteins
title_short Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
title_full Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
title_fullStr Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
title_full_unstemmed Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
title_sort Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
dc.creator.none.fl_str_mv Silva, Neusa Pereira da [UNIFESP]
Christofolini, Denise Maria [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
author Silva, Neusa Pereira da [UNIFESP]
author_facet Silva, Neusa Pereira da [UNIFESP]
Christofolini, Denise Maria [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
author_role author
author2 Christofolini, Denise Maria [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.subject.por.fl_str_mv Cajal body
Coiled body
Micronuclei
Cell nucleolus
Nucleolus organizer region
RNA polymerase I
Nucleolar proteins
topic Cajal body
Coiled body
Micronuclei
Cell nucleolus
Nucleolus organizer region
RNA polymerase I
Nucleolar proteins
description Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or two micronuclei containing nucleolus. By confocal microscopy we showed that in most micronuclei lacking a typical nucleolus a variable number of round structures were stained by antibodies against fibrillarin, NOR-90/hUBF protein, and coilin. These bodies were regarded as CB-like structures and were not stained by anti-PM/Scl and anti-To/Th antibodies. Anti-RNA polymerase I antibodies also reacted with CB-like structures in some micronuclei lacking nucleolus. The demonstration that a set of proteins involved in RNA/RNP biogenesis, namely coilin, fibrillarin, NOR-90/hUBF, and RNA polymerase I gather in CB-like structures present in nucleoli-devoid micronuclei may contribute to shed some light into the understanding of CB function.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-01
2015-06-14T13:31:12Z
2015-06-14T13:31:12Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2004000700008
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 997-1003, 2004.
10.1590/S0100-879X2004000700008
S0100-879X2004000700008.pdf
0100-879X
S0100-879X2004000700008
http://repositorio.unifesp.br/handle/11600/2156
WOS:000222450200008
dc.identifier.dark.fl_str_mv ark:/48912/0013000025qcc
url http://dx.doi.org/10.1590/S0100-879X2004000700008
http://repositorio.unifesp.br/handle/11600/2156
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 997-1003, 2004.
10.1590/S0100-879X2004000700008
S0100-879X2004000700008.pdf
0100-879X
S0100-879X2004000700008
WOS:000222450200008
ark:/48912/0013000025qcc
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 997-1003
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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score 15.301603

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