Cytotoxic effects of essential oils from three Lippia gracilis Schauer genotypes on HeLa, B16, and MCF-7 cells and normal human fibroblasts

This study aimed to evaluate the chemical composition of the essential oils from three genotypes of Lippia gracilis Schauer (Verbenaceae) and investigate the cytotoxic activities of these oils. Essential oils were extracted from the leaves using a Clevenger-type apparatus, and chemical analysis was...

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Detalles Bibliográficos
Autores: Melo, Juliana Oliveira de, Saltoratto, Ana Lucia Fachin, Rizo, Walace Fraga, Jesus, Hugo César Ramos de, Arrigoni-Blank, Maria de Fátima, Alves, Péricles Barreto, Marins, Mozart de Azevedo, França, Suzelei de Castro, Blank, Arie Fitzgerald
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Universidade Federal de Sergipe (UFS)
Repositorio:Repositório Institucional da UFS
Idioma:inglés
OAI Identifier:oai:oai:ri.ufs.br:repo_01:riufs/1635
Acceso en línea:https://ri.ufs.br/handle/riufs/1635
Access Level:acceso abierto
Palabra clave:Lippia gracilis
Germplasm
Volatile oil
Thymol
Carvacrol
Antitumor potential
Germoplasma
Óleo volátil
Descripción
Sumario:This study aimed to evaluate the chemical composition of the essential oils from three genotypes of Lippia gracilis Schauer (Verbenaceae) and investigate the cytotoxic activities of these oils. Essential oils were extracted from the leaves using a Clevenger-type apparatus, and chemical analysis was performed using a gas chromatograph coupled to a mass spectrometer and flame ionization detector. 3T3, MRC5, B16, HeLa, and MCF-7 cell lines were used to study the in vitro cytotoxicity of the essential oils, and the level of cell death was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test with three replicates. The cytotoxic activity was expressed as the concentration that inhibited 50% of cell growth. The main compound in the essential oil of LGRA-106 was thymol (40.52%), while LGRA-109 and LGRA-201 contained 45.84 and 32.60% carvacrol, respectively, as their major compound. The essential oils of L. gracilis showed cytotoxic activity against both normal and tumor cells at concentrations below 100 μg/mL; this demonstrated the antitumor potential of these essential oils, which should be further investigated.

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