Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family

Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit...

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Detalles Bibliográficos
Autores: Motta, Maria Cristina Machado, Azevedo Martins, Allan Cezar de, Souza, Silvana Sant'Anna de, Costa Catta-Preta, Carolina Moura, Silva, Rosane, Klein, Cecilia Coimbra, Paula de Almeida, Luiz Gonzaga, Cunha, Oberdan de Lima, Ciapina, Luciane Prioli, Brocchi, Marcelo, Colabardini, Ana Cristina, Lima, Bruna de Araujo, Machado, Carlos Renato, Almeida Soares, Celia Maria de, Probst, Christian Macagnan, Afonso de Menezes, Claudia Beatriz, Thompson, Claudia Elizabeth, Bartholomeu, Daniella Castanheira, Gradia, Daniela Fiori, Pavoni, Daniela Parada, Grisard, Edmundo C., Fantinatti-Garboggini, Fabiana, Marchini, Fabricio Klerynton, Rodrigues-Luiz, Gabriela Flaia, Wagner, Glauber, Goldman, Gustavo Henrique, Rangel Fietto, Juliana Lopes, Elias, Maria Carolina, Goldman, Maria Helena S., Sagot, Marie-France, Pereira, Maristela, Stoco, Patricia H., Mendonca-Neto, Rondon Pessoa de, Ribeiro Teixeira, Santuza Maria, Ferreira Maciel, Talles Eduardo, Oliveira Mendes, Tiago Antonio de, Uermenyi, Turan P., Souza, Wanderley de, Schenkman, Sergio [UNIFESP], Ribeiro de Vasconcelos, Ana Tereza
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/36195
Acceso en línea:http://dx.doi.org/10.1371/journal.pone.0060209
http://repositorio.unifesp.br/handle/11600/36195
Access Level:acceso abierto
Descripción
Sumario:Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. in an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. the monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. the monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. the assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution.

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