Microcystins in water samples from hemodialysis clinics of São Paulo state/Brazil

Cyanobacteria (blue-green algae) are most ancient photosynthetic prokaryotes organisms originated from pre-Cambrian era, which have adapted to several habitats, from thermal springs to humid ground. These organisms appear abundantly in fresh water forming blooms under determined ambient conditions,...

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Detalles Bibliográficos
Autores: Ruvieri, Valter, Shundo, Luzia, Alaburda, Janete, Sabino, Myrna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:Brasil
Institución:Instituto Adolfo Lutz
Repositorio:Revista do Instituto Adolfo Lutz (Online)
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.saude.sp.gov.br:article/34860
Acceso en línea:https://periodicos.saude.sp.gov.br/RIAL/article/view/34860
Access Level:acceso abierto
Palabra clave:cyanobacteria
microcystins
hemodialysis water
ELISA
toxins
cianobacterias
microcistinas
água de hemodiálise
toxinas
Descripción
Sumario:Cyanobacteria (blue-green algae) are most ancient photosynthetic prokaryotes organisms originated from pre-Cambrian era, which have adapted to several habitats, from thermal springs to humid ground. These organisms appear abundantly in fresh water forming blooms under determined ambient conditions, such as high luminosity, high temperature, and high nutrient concentration. Cyanobacteria produce some types of toxins such as microcystins (MC), which are one of the most important one due to its toxicity. Microcystins are heptapeptides formed by five invariable and two variable amino acids. More than 60 microcystins have been chemically identified. It has known that one of these toxins, the MC-LR (leucine, arginine) induces intracellular enzymes (phosphatases) inhibition, causing alteration in the cell structure. Sub-lethal doses of this hepatotoxin have been associated with malignancy development. The aim of the present study was to determine the  concentration of microcystins in water used for hemodialysis procedure. Water samples were collected from hemodialysis clinics located in the State of São Paulo - Brazil from April/2002 to March/2003, by means of routine official visit of the Sanitation Inspection Committee. Microcystins concentrations were determined by means of commercially available enzyme immunoassay [ELISA (Envirologix Inc)]. ELISA kit was tested , and a standard curve with...