Antibacterial and regenerative action of fibrin rich in platelets and leukocytes
Introduction: Regenerative medicine involves, among other biostimulating strategies, the use of blood derivatives in order to regulate and accelerate the healing process. Fibrin rich in platelets and leukocytes (L-PRF) is a resultant platelet with 3 by-products: acellular supernatant serum (SAS), cl...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | Brasil |
| Institución: | Universidade de Araraquara (UNIARA) |
| Repositorio: | Revista Brasileira Multidisciplinar |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.revistarebram.com:article/2073 |
| Acceso en línea: | http://revistarebram.com/index.php/revistauniara/article/view/2073 |
| Access Level: | acceso abierto |
| Palabra clave: | Medicina regenerativa Biomodulação Fibrina Cicatrização Antimicrobiano Regenerative medicine Biomodulation Fibrin Healing Anti-bacterial |
| Sumario: | Introduction: Regenerative medicine involves, among other biostimulating strategies, the use of blood derivatives in order to regulate and accelerate the healing process. Fibrin rich in platelets and leukocytes (L-PRF) is a resultant platelet with 3 by-products: acellular supernatant serum (SAS), clot and exudate. Despite being promising, there are few studies that characterize the real influence of this platelet concentrate on the repair of cutaneous wounds. Objective: To evaluate, within an in vitro investigative approach, through antibacterial analysis and cytokine and growth factor dosage, the immunomodulatory influence of L-PRF. Method: 10 clinically healthy volunteers were selected. Some women practiced physical activity daily and others weekly. To obtain the L-PRF derivatives, a blood sample was collected, with the amount of 4 tubes of 5 mL of blood from each candidate. Results: The evaluation of the potential antibacterial properties of the volunteers, on Staphylococcus aureus and Pseudomonas aeruginosa, showed positive action in 40% of the total L-PRF by-products. There was selectivity and differentiation, in the intrinsic individuality of each volunteer, when comparing the 3 elements of L-PRF in the inhibition of the growth zone. Then came another stage of the present study, the dosage of cytokines and growth factor, respectively: tumor necrosis factor (TNF), interleukin 10 (IL-10) and transforming growth factor b (TGF-β) in SAS and exudate. Conclusion: In view of the determination of the antibacterial effect of the by-products of L-PRF, exudate and clot, promising results are also evident, in relation to the biomodulatory effect evidenced in this last step, when comparing the SAS and exudate. |
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