In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay

There is high interest in the natural products properties due to their use in popular medicine. Agaricus blazei Murrill ss. Heinem. (Ab) is native to Brazil and has been widely disseminated because its medicinal properties. In the present study, the genotoxic and antigenotoxic potential of Ab extrac...

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Detalhes bibliográficos
Autores: Bellini, Marilanda Ferreira, Terezan, Ana Paula, Ribeiro, Lúcia Regina [UNESP], Mantovani, Mário Sérgio
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/69062
Acesso em linha:http://dx.doi.org/10.1508/cytologia.71.205
http://hdl.handle.net/11449/69062
Access Level:acceso abierto
Palavra-chave:Agaricus blazei
Antigenotoxicity
Comet assay
Genotoxicity
Metabolism
Cricetulus griseus
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spelling In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assayAgaricus blazeiAntigenotoxicityComet assayGenotoxicityMetabolismCricetulus griseusThere is high interest in the natural products properties due to their use in popular medicine. Agaricus blazei Murrill ss. Heinem. (Ab) is native to Brazil and has been widely disseminated because its medicinal properties. In the present study, the genotoxic and antigenotoxic potential of Ab extracts were investigated using the comet assay. The cells utilized were the non drug-metabolizing line CHO-k1 (Chinese hamster ovary) and the drug-metabolizing line HTC (rat hepatoma). Cells were treated for 3 h in the absence of fetal bovain serum (FBS) with methanolic, hexanic and n-butanolic extracts at 50 μg/ml and 0.75% aqueous extract to test for genotoxicity. Antigenotoxic effects of extracts were determined in cells exposed to the DNA damage inducing agent ethyl methanesulfonate under simultaneous or simultaneous with 1 h pre-incubation conditions. The extracts did not show genotoxicity in HTC, while they were genotoxic in CHO-k1. No antigenotoxic effect was observed with any extract under any condition. These results demonstrate that the metabolism in presence or in absence has a direct influence on the genotoxicity of these extracts. © 2006 The Japan Mendel Society.Departamento de Biologia Geral Universidade Estadual de Londrina, Londrina-PRDepartamento de Química Universidade Federal de São Carlos-UFSCar, São Carlos-SPDepartamento de Patologia UNESP, Botucatu-SPDepartamento de Patologia UNESP, Botucatu-SPUniversidade Estadual de Londrina (UEL)Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)2014-05-27T11:21:57Z2014-05-27T11:21:57Z2006-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article205-211application/pdfhttp://dx.doi.org/10.1508/cytologia.71.205Cytologia, v. 71, n. 3, p. 205-211, 2006.0011-4545http://hdl.handle.net/11449/6906210.1508/cytologia.71.2052-s2.0-337507380932-s2.0-33750738093.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytologia0.4810,233info:eu-repo/semantics/openAccessBellini, Marilanda FerreiraTerezan, Ana PaulaRibeiro, Lúcia Regina [UNESP]Mantovani, Mário Sérgio2025-10-22T05:11:35Zoai:repositorio.unesp.br:11449/69062Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T05:11:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
title In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
spellingShingle In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
Bellini, Marilanda Ferreira
Agaricus blazei
Antigenotoxicity
Comet assay
Genotoxicity
Metabolism
Cricetulus griseus
title_short In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
title_full In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
title_fullStr In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
title_full_unstemmed In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
title_sort In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
dc.creator.none.fl_str_mv Bellini, Marilanda Ferreira
Terezan, Ana Paula
Ribeiro, Lúcia Regina [UNESP]
Mantovani, Mário Sérgio
author Bellini, Marilanda Ferreira
author_facet Bellini, Marilanda Ferreira
Terezan, Ana Paula
Ribeiro, Lúcia Regina [UNESP]
Mantovani, Mário Sérgio
author_role author
author2 Terezan, Ana Paula
Ribeiro, Lúcia Regina [UNESP]
Mantovani, Mário Sérgio
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv Agaricus blazei
Antigenotoxicity
Comet assay
Genotoxicity
Metabolism
Cricetulus griseus
topic Agaricus blazei
Antigenotoxicity
Comet assay
Genotoxicity
Metabolism
Cricetulus griseus
description There is high interest in the natural products properties due to their use in popular medicine. Agaricus blazei Murrill ss. Heinem. (Ab) is native to Brazil and has been widely disseminated because its medicinal properties. In the present study, the genotoxic and antigenotoxic potential of Ab extracts were investigated using the comet assay. The cells utilized were the non drug-metabolizing line CHO-k1 (Chinese hamster ovary) and the drug-metabolizing line HTC (rat hepatoma). Cells were treated for 3 h in the absence of fetal bovain serum (FBS) with methanolic, hexanic and n-butanolic extracts at 50 μg/ml and 0.75% aqueous extract to test for genotoxicity. Antigenotoxic effects of extracts were determined in cells exposed to the DNA damage inducing agent ethyl methanesulfonate under simultaneous or simultaneous with 1 h pre-incubation conditions. The extracts did not show genotoxicity in HTC, while they were genotoxic in CHO-k1. No antigenotoxic effect was observed with any extract under any condition. These results demonstrate that the metabolism in presence or in absence has a direct influence on the genotoxicity of these extracts. © 2006 The Japan Mendel Society.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
2014-05-27T11:21:57Z
2014-05-27T11:21:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1508/cytologia.71.205
Cytologia, v. 71, n. 3, p. 205-211, 2006.
0011-4545
http://hdl.handle.net/11449/69062
10.1508/cytologia.71.205
2-s2.0-33750738093
2-s2.0-33750738093.pdf
url http://dx.doi.org/10.1508/cytologia.71.205
http://hdl.handle.net/11449/69062
identifier_str_mv Cytologia, v. 71, n. 3, p. 205-211, 2006.
0011-4545
10.1508/cytologia.71.205
2-s2.0-33750738093
2-s2.0-33750738093.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cytologia
0.481
0,233
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 205-211
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300719

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