Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum

BACKGROUND: The gastrointestinal disorders have been associated with morphological alterations in the myenteric nervous plexus. AIM: To evaluate, through morphometric studies, the chronic effects of the subdiaphragmatic trunk vagotomy on the nervous plexus. METHODS: Fifteen male exemplars of Wistar...

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Detalles Bibliográficos
Autores: Martins-Júnior, Carlos Roberto, Magalhães-Júnior, Aezio de, Inagaki, Paola Mayumi, Pires, Pedro Paulo, Lachat, João José, Silva, Jesiel Mamedes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Brasil
Institución:Universidade Federal de Mato Grosso do Sul (UFMS)
Repositorio:Repositório Institucional da UFMS
Idioma:inglés
OAI Identifier:oai:repositorio.ufms.br:123456789/148
Acceso en línea:https://repositorio.ufms.br/handle/123456789/148
Access Level:acceso abierto
Palabra clave:Denervação Autônoma
Plexo Mientérico
Autonomic Denervation
Myenteric Plexus
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dc.title.pt_BR.fl_str_mv Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
dc.title.alternative.pt_BR.fl_str_mv Efeito crônico da vagotomia sobre a morfometria do plexo mioentérico do duodeno de ratos
title Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
spellingShingle Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
Martins-Júnior, Carlos Roberto
Denervação Autônoma
Plexo Mientérico
Autonomic Denervation
Myenteric Plexus
title_short Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
title_full Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
title_fullStr Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
title_full_unstemmed Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
title_sort Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum
dc.creator.none.fl_str_mv Martins-Júnior, Carlos Roberto
Magalhães-Júnior, Aezio de
Inagaki, Paola Mayumi
Pires, Pedro Paulo
Lachat, João José
Silva, Jesiel Mamedes
author Martins-Júnior, Carlos Roberto
author_facet Martins-Júnior, Carlos Roberto
Magalhães-Júnior, Aezio de
Inagaki, Paola Mayumi
Pires, Pedro Paulo
Lachat, João José
Silva, Jesiel Mamedes
author_role author
author2 Magalhães-Júnior, Aezio de
Inagaki, Paola Mayumi
Pires, Pedro Paulo
Lachat, João José
Silva, Jesiel Mamedes
author2_role author
author
author
author
author
dc.subject.por.fl_str_mv Denervação Autônoma
Plexo Mientérico
Autonomic Denervation
Myenteric Plexus
topic Denervação Autônoma
Plexo Mientérico
Autonomic Denervation
Myenteric Plexus
description BACKGROUND: The gastrointestinal disorders have been associated with morphological alterations in the myenteric nervous plexus. AIM: To evaluate, through morphometric studies, the chronic effects of the subdiaphragmatic trunk vagotomy on the nervous plexus. METHODS: Fifteen male exemplars of Wistar Rattus novergicus weighing about 150g, distributed into three groups, have been used: control (n=5), Sham (n=5) and vagotomized (n=5). The animals were sacrificed after 30 and 90 days post surgery. Fragments of duodenum were fixed in Bouin solution, embedded into paraffin and stained with HE and PAS. Morphometric analysis was performed by a Carl Zeiss KM 450 image system. The following aspects were observed: the density of nervous cells per linear micrometer (µm) (ND); the area of perikarya (µm2) (NA); the number of satellite cells per µm (SCD); and the number of satellite cells per neuron (SC/N). The averages were compared with the help of "software" program Sigma Plus through two way - ANOVA and Tuckey post-test. RESULTS: Denervation increased SC/N (p<0,05) and NA (p<0,05), in a time-dependent denervation way (p<0,05). However ND and SCD, decreased, which significantly with the animal's age (p<0,001). CONCLUSION: Vagotomy altered the myenteric plexus morphology in a time-dependent way.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2011-07-20T18:20:55Z
dc.date.available.fl_str_mv 2021-09-30T19:55:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS-JUNIOR, Carlos Roberto et al . Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum. ABCD, arq. bras. cir. dig., São Paulo, v. 23, n. 3, Sept. 2010 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202010000300006&lng=en&nrm=iso>. access on 20 July 2011. http://dx.doi.org/10.1590/S0102-67202010000300006.
dc.identifier.uri.fl_str_mv https://repositorio.ufms.br/handle/123456789/148
dc.identifier.doi.none.fl_str_mv 10.1590/S0102-67202010000300006
identifier_str_mv MARTINS-JUNIOR, Carlos Roberto et al . Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum. ABCD, arq. bras. cir. dig., São Paulo, v. 23, n. 3, Sept. 2010 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202010000300006&lng=en&nrm=iso>. access on 20 July 2011. http://dx.doi.org/10.1590/S0102-67202010000300006.
10.1590/S0102-67202010000300006
url https://repositorio.ufms.br/handle/123456789/148
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
publisher.none.fl_str_mv ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMS
instname:Universidade Federal de Mato Grosso do Sul (UFMS)
instacron:UFMS
instname_str Universidade Federal de Mato Grosso do Sul (UFMS)
instacron_str UFMS
institution UFMS
reponame_str Repositório Institucional da UFMS
collection Repositório Institucional da UFMS
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spelling 2011-07-20T18:20:55Z2021-09-30T19:55:10Z2010MARTINS-JUNIOR, Carlos Roberto et al . Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum. ABCD, arq. bras. cir. dig., São Paulo, v. 23, n. 3, Sept. 2010 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202010000300006&lng=en&nrm=iso>. access on 20 July 2011. http://dx.doi.org/10.1590/S0102-67202010000300006.https://repositorio.ufms.br/handle/123456789/14810.1590/S0102-67202010000300006BACKGROUND: The gastrointestinal disorders have been associated with morphological alterations in the myenteric nervous plexus. AIM: To evaluate, through morphometric studies, the chronic effects of the subdiaphragmatic trunk vagotomy on the nervous plexus. METHODS: Fifteen male exemplars of Wistar Rattus novergicus weighing about 150g, distributed into three groups, have been used: control (n=5), Sham (n=5) and vagotomized (n=5). The animals were sacrificed after 30 and 90 days post surgery. Fragments of duodenum were fixed in Bouin solution, embedded into paraffin and stained with HE and PAS. Morphometric analysis was performed by a Carl Zeiss KM 450 image system. The following aspects were observed: the density of nervous cells per linear micrometer (µm) (ND); the area of perikarya (µm2) (NA); the number of satellite cells per µm (SCD); and the number of satellite cells per neuron (SC/N). The averages were compared with the help of "software" program Sigma Plus through two way - ANOVA and Tuckey post-test. RESULTS: Denervation increased SC/N (p<0,05) and NA (p<0,05), in a time-dependent denervation way (p<0,05). However ND and SCD, decreased, which significantly with the animal's age (p<0,001). CONCLUSION: Vagotomy altered the myenteric plexus morphology in a time-dependent way.RACIONAL: As disfunções gastrintestinais têm sido associadas à alterações morfológicas no plexo nervoso mioentérico. OBJETIVO: Avaliar através do estudo morfométrico, os efeitos crônicos da vagotomia troncular subdiafragmática sobre esse plexo nervoso. MÉTODOS: Foram utilizados 15 exemplares machos de Rattus novergicus da variedade Wistar, com cerca de 150 g, distribuídos nos grupos controle (n=5), Sham (n=5) e vagotomizados (n=5). Os animais foram sacrificados depois de 30 e 90 dias após as operações. Em seguida, fragmentos do duodeno foram fixados em solução de Bouin, incluídos em parafina e corados por HE e PAS. A análise morfométrica foi realizada por meio do sistema de análise de imagem Carl Zeiss KM 450. Foram observados: a densidade de células nervosas por micrômetro linear (µm); a área dos pericários (µm2); o número de células satélites por µm; e o número de células satélites por neurônio. As médias foram comparadas com o auxílio do programa de "software" Sigma Plus através do Two way - ANOVA e do pós-teste de Tukey. RESULTADOS: A desnervação aumentou o número de células satélites por neurônios (p<0,05) e a área média dos pericários (p<0,05), de maneira dependente do tempo de desnervação (p<0,05), mas diminuiu significativamente a densidade de neurônios (p<0,05) e de células satélites (p<0,05) em função da idade (p<0,001). CONCLUSÃO: A vagotomia alterou a morfologia do plexo mioentérico de maneira dependente do tempo.engABCD. 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