Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model

This study aimed to evaluate the carcinogenic potential of the herbicide Diuron in a two-stage rat medium-term mammary carcinogenesis model initiated by 7,12-dimethylbenz(a)anthracene (DMBA). Female seven-week-old Sprague-Dawley (SD) rats were allocated to six groups: groups G1 to G4 received intrag...

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Detalles Bibliográficos
Autores: Grassi, Tony Fernando [UNESP], Rodrigues, Maria Aparecida Marchesan [UNESP], Camargo, João Lauro Viana de [UNESP], Barbisan, Luis Fernando [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/12895
Acceso en línea:http://dx.doi.org/10.1177/0192623310396904
http://hdl.handle.net/11449/12895
Access Level:acceso abierto
Palabra clave:pesticides
diuron
mammary carcinogenesis
Sprague-Dawley rats
toxicology
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spelling Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Modelpesticidesdiuronmammary carcinogenesisSprague-Dawley ratstoxicologyThis study aimed to evaluate the carcinogenic potential of the herbicide Diuron in a two-stage rat medium-term mammary carcinogenesis model initiated by 7,12-dimethylbenz(a)anthracene (DMBA). Female seven-week-old Sprague-Dawley (SD) rats were allocated to six groups: groups G1 to G4 received intragastrically (i.g.) a single 50 mg/kg dose of DMBA; groups G5 and G6 received single administration of canola oil (vehicle of DMBA). Groups G1 and G5 received a basal diet, and groups G2, G3, G4, and G6 were fed the basal diet with the addition of Diuron at 250, 1250, 2500, and 2500 ppm, respectively. After twenty-five weeks, the animals were euthanized and mammary tumors were histologically confirmed and quantified. Tumor samples were also processed for immunohistochemical evaluation of the expressions of proliferating cell nuclear antigen (PCNA), cleaved caspase-3, estrogen receptor-alpha (ER-alpha), p63, bcl-2, and bak. Diuron treatment did not increase the incidence or multiplicity of mammary tumors (groups G2 to G4 versus Group G1). Also, exposure to Diuron did not alter tumor growth (cell proliferation and apoptosis indexes) or immunoreactivity to ER-alpha, p63 (myoephitelial marker), or bcl-2 and bak (apoptosis regulatory proteins). These findings indicate that Diuron does not have a promoting potential on mammary carcinogenesis in female SD rats initiated with DMBA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)TOXICAM (Centre of the Evaluation of the Impact of the Environment on Human Health, Department of Pathology - Botucatu Medical School, UNESP, Brazil)UNESP São Paulo State Univ, Inst Biosci, Dept Morphol, Botucatu, SP, BrazilUNESP São Paulo State Univ, Dept Pathol, Sch Med, Botucatu, SP, BrazilUNESP São Paulo State Univ, Inst Biosci, Dept Morphol, Botucatu, SP, BrazilUNESP São Paulo State Univ, Dept Pathol, Sch Med, Botucatu, SP, BrazilFAPESP: 06/01330-0Sage Publications IncUniversidade Estadual Paulista (Unesp)2014-05-20T13:37:19Z2014-05-20T13:37:19Z2011-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article486-495http://dx.doi.org/10.1177/0192623310396904Toxicologic Pathology. Thousand Oaks: Sage Publications Inc, v. 39, n. 3, p. 486-495, 2011.0192-6233http://hdl.handle.net/11449/1289510.1177/0192623310396904WOS:0002933801000043278528112652257Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicologic Pathology1.9660,807info:eu-repo/semantics/openAccessGrassi, Tony Fernando [UNESP]Rodrigues, Maria Aparecida Marchesan [UNESP]Camargo, João Lauro Viana de [UNESP]Barbisan, Luis Fernando [UNESP]2025-10-24T03:02:46Zoai:repositorio.unesp.br:11449/12895Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-24T03:02:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
title Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
spellingShingle Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
Grassi, Tony Fernando [UNESP]
pesticides
diuron
mammary carcinogenesis
Sprague-Dawley rats
toxicology
title_short Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
title_full Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
title_fullStr Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
title_full_unstemmed Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
title_sort Evaluation of Carcinogenic Potential of Diuron in a Rat Mammary Two-Stage Carcinogenesis Model
dc.creator.none.fl_str_mv Grassi, Tony Fernando [UNESP]
Rodrigues, Maria Aparecida Marchesan [UNESP]
Camargo, João Lauro Viana de [UNESP]
Barbisan, Luis Fernando [UNESP]
author Grassi, Tony Fernando [UNESP]
author_facet Grassi, Tony Fernando [UNESP]
Rodrigues, Maria Aparecida Marchesan [UNESP]
Camargo, João Lauro Viana de [UNESP]
Barbisan, Luis Fernando [UNESP]
author_role author
author2 Rodrigues, Maria Aparecida Marchesan [UNESP]
Camargo, João Lauro Viana de [UNESP]
Barbisan, Luis Fernando [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv pesticides
diuron
mammary carcinogenesis
Sprague-Dawley rats
toxicology
topic pesticides
diuron
mammary carcinogenesis
Sprague-Dawley rats
toxicology
description This study aimed to evaluate the carcinogenic potential of the herbicide Diuron in a two-stage rat medium-term mammary carcinogenesis model initiated by 7,12-dimethylbenz(a)anthracene (DMBA). Female seven-week-old Sprague-Dawley (SD) rats were allocated to six groups: groups G1 to G4 received intragastrically (i.g.) a single 50 mg/kg dose of DMBA; groups G5 and G6 received single administration of canola oil (vehicle of DMBA). Groups G1 and G5 received a basal diet, and groups G2, G3, G4, and G6 were fed the basal diet with the addition of Diuron at 250, 1250, 2500, and 2500 ppm, respectively. After twenty-five weeks, the animals were euthanized and mammary tumors were histologically confirmed and quantified. Tumor samples were also processed for immunohistochemical evaluation of the expressions of proliferating cell nuclear antigen (PCNA), cleaved caspase-3, estrogen receptor-alpha (ER-alpha), p63, bcl-2, and bak. Diuron treatment did not increase the incidence or multiplicity of mammary tumors (groups G2 to G4 versus Group G1). Also, exposure to Diuron did not alter tumor growth (cell proliferation and apoptosis indexes) or immunoreactivity to ER-alpha, p63 (myoephitelial marker), or bcl-2 and bak (apoptosis regulatory proteins). These findings indicate that Diuron does not have a promoting potential on mammary carcinogenesis in female SD rats initiated with DMBA.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
2014-05-20T13:37:19Z
2014-05-20T13:37:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1177/0192623310396904
Toxicologic Pathology. Thousand Oaks: Sage Publications Inc, v. 39, n. 3, p. 486-495, 2011.
0192-6233
http://hdl.handle.net/11449/12895
10.1177/0192623310396904
WOS:000293380100004
3278528112652257
url http://dx.doi.org/10.1177/0192623310396904
http://hdl.handle.net/11449/12895
identifier_str_mv Toxicologic Pathology. Thousand Oaks: Sage Publications Inc, v. 39, n. 3, p. 486-495, 2011.
0192-6233
10.1177/0192623310396904
WOS:000293380100004
3278528112652257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicologic Pathology
1.966
0,807
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 486-495
dc.publisher.none.fl_str_mv Sage Publications Inc
publisher.none.fl_str_mv Sage Publications Inc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300724

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