Detection of enterotoxin genes of Staphylococcus sp isolated from nasal cavities and hands of food handlers

Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%)...

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Detalles Bibliográficos
Autores: Rall, V. L. M. [UNESP], Sforcin, J. M. [UNESP], Augustini, V. C. M. [UNESP], Watanabe, M. T. [UNESP], Fernandes Jr., A. [UNESP], Rall, R., Silva, M. G. [UNESP], Araújo Jr., J. P. [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/18127
Acceso en línea:http://dx.doi.org/10.1590/S1517-83822010000100011
http://hdl.handle.net/11449/18127
Access Level:acceso abierto
Palabra clave:Staphylococcal enterotoxins
coagulase-negative staphylococci
S. aureus
food handler
Descripción
Sumario:Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%) and 20 CPS strains (24.4%). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotoxins was high (46.8%). Despite of the staphylococcal species, the most common gene was sea (35.4%), followed by seh and sej (29.2%). The detection of new staphylococcal enterotoxins (SEs) genes showed a higher pathogenic potential in this genus. The presence of these gene points out the importance of CNS not only as contaminant bacteria but also as a pathogen.