Protective effect of N-acetyl-L-cysteine and rosuvastatin against oxidative stress in fibroblasts from asymptomatic patients with X-ALD : a preliminary study

Introduction: Several studies in the literature have evaluated the role of oxidative stress and adjuvant therapies for X-linked adrenoleukodystrophy (X-ALD). Here, we investigated whether n-acetyl-L-cysteine (NAC) and rosuvastatin (RSV) could influence the generation of reactive species, redox statu...

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Detalhes bibliográficos
Autores: Marchetti, Desirèe Padilha, Ribas, Graziela de Oliveira Schmitt, Donida, Bruna, Brito, Verônica Bidinotto, Moura, Dinara Jaqueline, Coelho, Daniella de Moura, Machado, Andryele Zaffari, Kessler, Rejane Gus, Coitinho, Adriana Simon, Vargas, Carmen Regla
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Recursos:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/225215
Acesso em linha:http://hdl.handle.net/10183/225215
Access Level:acceso abierto
Palavra-chave:Estresse oxidativo
Acetilcisteína
Adrenoleucodistrofia
Rosuvastatina cálcica
X-linked adrenoleukodystrophy
Fibroblasts
N-acetyl-L-cysteine
Rosuvastatin
Oxidative stress
Descrição
Resumo:Introduction: Several studies in the literature have evaluated the role of oxidative stress and adjuvant therapies for X-linked adrenoleukodystrophy (X-ALD). Here, we investigated whether n-acetyl-L-cysteine (NAC) and rosuvastatin (RSV) could influence the generation of reactive species, redox status and nitrative stress in fibroblasts from asymptomatic patients with X-ALD. Methods: Skin biopsy samples were cultured and treated for 2 hours (37 °C) with NAC and RSV. Results: X-ALD fibroblasts generated high levels of reactive oxygen species. These levels were significantly lower in fibroblasts treated with NAC and RSV relative to untreated samples. The X-ALD fibroblasts from asymptomatic patients also had higher catalase activity, and only NAC was able to increase enzyme activity in the samples. Conclusions: Our results indicated that NAC and RSV were able to improve oxidative stress parameters in fibroblasts from asymptomatic patients with X-ALD, showing that adjuvant antioxidant therapy may be a promising treatment strategy for asymptomatic patients with this disease.

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