Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates

This study identified Staphylococcus aureus and the methicillin-resistant (MRSA) strains in raw milk samples using molecular methods, and their minimum inhibitory concentrations (MIC) were evaluated. Two hundred fifty-one samples of raw milk samples from bulk tanks, collected in the state of Minas G...

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Autores: Teixeira, Junia Pacheco, Silva, Nivaldo, Fonseca, Leorges Moraes da, Costa, Geraldo Marcio da
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Brasil
Recursos:Instituto Adolfo Lutz
Repositorio:Revista do Instituto Adolfo Lutz (Online)
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.saude.sp.gov.br:article/33348
Acesso em linha:https://periodicos.saude.sp.gov.br/RIAL/article/view/33348
Access Level:acceso abierto
Palavra-chave:minimum inhibitory concentration
femA
mecA
Staphylococcus aureus
methicillin resistance
concentração inibitória mínima
resistência à meticilina
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oai_identifier_str oai:ojs.periodicos.saude.sp.gov.br:article/33348
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
Uso de PCR Duplex para detecção dos genes femA e mecA e determinação da concentração inibitória mínima (CIM) em Staphylococcus aureus isolados de leite cru
title Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
spellingShingle Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
Teixeira, Junia Pacheco
minimum inhibitory concentration
femA
mecA
Staphylococcus aureus
methicillin resistance
concentração inibitória mínima
femA
mecA
Staphylococcus aureus
resistência à meticilina
title_short Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
title_full Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
title_fullStr Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
title_full_unstemmed Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
title_sort Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolates
dc.creator.none.fl_str_mv Teixeira, Junia Pacheco
Silva, Nivaldo
Fonseca, Leorges Moraes da
Costa, Geraldo Marcio da
author Teixeira, Junia Pacheco
author_facet Teixeira, Junia Pacheco
Silva, Nivaldo
Fonseca, Leorges Moraes da
Costa, Geraldo Marcio da
author_role author
author2 Silva, Nivaldo
Fonseca, Leorges Moraes da
Costa, Geraldo Marcio da
author2_role author
author
author
dc.subject.por.fl_str_mv minimum inhibitory concentration
femA
mecA
Staphylococcus aureus
methicillin resistance
concentração inibitória mínima
femA
mecA
Staphylococcus aureus
resistência à meticilina
topic minimum inhibitory concentration
femA
mecA
Staphylococcus aureus
methicillin resistance
concentração inibitória mínima
femA
mecA
Staphylococcus aureus
resistência à meticilina
description This study identified Staphylococcus aureus and the methicillin-resistant (MRSA) strains in raw milk samples using molecular methods, and their minimum inhibitory concentrations (MIC) were evaluated. Two hundred fifty-one samples of raw milk samples from bulk tanks, collected in the state of Minas Gerais, Brazil were analyzed using polymerase chain reaction (PCR) to investigate the presence of femA gene in 278 S.aureus isolates. Furthermore, MRSA strains were investigated for detecting mecA gene. All of 278 isolates contained the femA gene (132 bp), and in 11 samples (3.95 %) the mecA gene (533 bp) was identified. The minimum inhibitory concentration against S. aureus was determined using enrofloxacin, trimethoprim-sulfamethoxazole, gentamicin, azithromycin, tetracycline, cephalothin, amoxicillin, penicillin G and lincomycin. Enrofloxacin and trimethoprim-sulfamethoxazole showed to be the most effective inhibitors, as this study identified 149 of 161 isolates (92.5 % of susceptibility) with MIC≤ 4 mg/ mL, and 55 of 66 isolates (83.3 % of susceptibility) with MIC≤ 2 mg/mL, for the first antimicrobial drug and the second one, respectively.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/33348
10.18241/0073-98552014731615
url https://periodicos.saude.sp.gov.br/RIAL/article/view/33348
identifier_str_mv 10.18241/0073-98552014731615
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/33348/32180
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 73 No. 3 (2014); 272-279
Revista del Instituto Adolfo Lutz - RIAL; Vol. 73 Núm. 3 (2014); 272-279
Revista do Instituto Adolfo Lutz; v. 73 n. 3 (2014); 272-279
1983-3814
0073-9855
10.18241/rial.v73i3
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
_version_ 1853663533821067264
spelling Detection of femA and mecA genes in Staphylococcus aureus isolated from bovine raw milk using duplex PCR and determination of the minimum inhibitory concentration of the isolatesUso de PCR Duplex para detecção dos genes femA e mecA e determinação da concentração inibitória mínima (CIM) em Staphylococcus aureus isolados de leite cruminimum inhibitory concentrationfemAmecAStaphylococcus aureusmethicillin resistanceconcentração inibitória mínimafemAmecAStaphylococcus aureusresistência à meticilinaThis study identified Staphylococcus aureus and the methicillin-resistant (MRSA) strains in raw milk samples using molecular methods, and their minimum inhibitory concentrations (MIC) were evaluated. Two hundred fifty-one samples of raw milk samples from bulk tanks, collected in the state of Minas Gerais, Brazil were analyzed using polymerase chain reaction (PCR) to investigate the presence of femA gene in 278 S.aureus isolates. Furthermore, MRSA strains were investigated for detecting mecA gene. All of 278 isolates contained the femA gene (132 bp), and in 11 samples (3.95 %) the mecA gene (533 bp) was identified. The minimum inhibitory concentration against S. aureus was determined using enrofloxacin, trimethoprim-sulfamethoxazole, gentamicin, azithromycin, tetracycline, cephalothin, amoxicillin, penicillin G and lincomycin. Enrofloxacin and trimethoprim-sulfamethoxazole showed to be the most effective inhibitors, as this study identified 149 of 161 isolates (92.5 % of susceptibility) with MIC≤ 4 mg/ mL, and 55 of 66 isolates (83.3 % of susceptibility) with MIC≤ 2 mg/mL, for the first antimicrobial drug and the second one, respectively.O objetivo deste trabalho foi de identificar Staphylococcus aureus e as linhagens resistentes a meticilina (MRSA) em amostras de leite cru bovino, por meio de métodos moleculares, e de determinar a concentração inibitória mínima (CIM) dos isolados. S. aureus pode apresentar linhagens resistentes a meticilina e a outros antimicrobianos utilizados na pratica medico-veterinária. Foram analisadas 251 amostras de leite cru de tanques de expansão, procedentes de cinco mesorregiões produtoras de leite de Minas Gerais – Brasil. Por meio de PCR foi identificada a presença do gene femA em 278 isolados de S. aureus; e as linhagens MRSA foram determinadas pela presença do gene mecA. Os ensaios de PCR foram positivos para gene femA em 100 % dos isolados testados; e entre essas amostras 11 (3,95 %) apresentaram produto amplificado de 533 pb correspondente ao gene mecA. Para analisar a susceptibilidade dos isolados foi realizado o teste de CIM para nove substancias antimicrobianas. Foram identificados 149 isolados de S. aureus que apresentaram CIM≤ 4 μg/mL para enrofloxacina, com 92,54 % de susceptibilidade, e 55 isolados testados para sulfametoxazol+trimetoprima, apresentaram CIM ≤ 2 μg/mL com 83,33 % de susceptibilidade. Estes antimicrobianos foram considerados os mais eficazes.Instituto Adolfo Lutz2015-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3334810.18241/0073-98552014731615Instituto Adolfo Lutz Journal - RIAL; Vol. 73 No. 3 (2014); 272-279Revista del Instituto Adolfo Lutz - RIAL; Vol. 73 Núm. 3 (2014); 272-279Revista do Instituto Adolfo Lutz; v. 73 n. 3 (2014); 272-2791983-38140073-985510.18241/rial.v73i3reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/33348/32180Copyright (c) 2015 Junia Pacheco Teixeira, Nivaldo Silva, Leorges Moraes da Fonseca, Geraldo Marcio da Costainfo:eu-repo/semantics/openAccessTeixeira, Junia PachecoSilva, NivaldoFonseca, Leorges Moraes daCosta, Geraldo Marcio da2023-11-07T19:38:41Zoai:ojs.periodicos.saude.sp.gov.br:article/33348Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2023-11-07T19:38:41Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
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