The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis

Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.

Detalles Bibliográficos
Autores: Silva, Djane Clarys Baia da, Alvarez, Luis Carlos Salazar, Lizcano, Omaira Vera, Costa, Fabio Trindade Maranhão, Lopes, Stefanie Costa Pinto, Orfanó, Alessandra Silva, Pascoal, Denner Oliveira, Pimenta, Rafael Nacif, Rodriguez, Iria Cabral, Guerra, Maria das Graças Vale Barbosa, Lacerda, Marcus Vinícius Guimarães, Secundino, Nágila Francinete Costa, Monteiro, Wuelton Marcelo, Pimenta, Paulo Filemon Paolucci
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/33677
Acceso en línea:https://arca.fiocruz.br/handle/icict/33677
Access Level:acceso abierto
Palabra clave:Quitinase
hematocrito
Malária
Matriz peritrófica
Plasmodium vivax
Trypsin
Chitinase
Hematocrit
Malaria
Peritrophic matrix
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dc.title.none.fl_str_mv The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
title The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
spellingShingle The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
Silva, Djane Clarys Baia da
Quitinase
hematocrito
Malária
Matriz peritrófica
Plasmodium vivax
Trypsin
Chitinase
Hematocrit
Malaria
Peritrophic matrix
Plasmodium vivax
Trypsin
title_short The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
title_full The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
title_fullStr The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
title_full_unstemmed The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
title_sort The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
dc.creator.none.fl_str_mv Silva, Djane Clarys Baia da
Alvarez, Luis Carlos Salazar
Lizcano, Omaira Vera
Costa, Fabio Trindade Maranhão
Lopes, Stefanie Costa Pinto
Orfanó, Alessandra Silva
Pascoal, Denner Oliveira
Pimenta, Rafael Nacif
Rodriguez, Iria Cabral
Guerra, Maria das Graças Vale Barbosa
Lacerda, Marcus Vinícius Guimarães
Secundino, Nágila Francinete Costa
Monteiro, Wuelton Marcelo
Pimenta, Paulo Filemon Paolucci
author Silva, Djane Clarys Baia da
author_facet Silva, Djane Clarys Baia da
Alvarez, Luis Carlos Salazar
Lizcano, Omaira Vera
Costa, Fabio Trindade Maranhão
Lopes, Stefanie Costa Pinto
Orfanó, Alessandra Silva
Pascoal, Denner Oliveira
Pimenta, Rafael Nacif
Rodriguez, Iria Cabral
Guerra, Maria das Graças Vale Barbosa
Lacerda, Marcus Vinícius Guimarães
Secundino, Nágila Francinete Costa
Monteiro, Wuelton Marcelo
Pimenta, Paulo Filemon Paolucci
author_role author
author2 Alvarez, Luis Carlos Salazar
Lizcano, Omaira Vera
Costa, Fabio Trindade Maranhão
Lopes, Stefanie Costa Pinto
Orfanó, Alessandra Silva
Pascoal, Denner Oliveira
Pimenta, Rafael Nacif
Rodriguez, Iria Cabral
Guerra, Maria das Graças Vale Barbosa
Lacerda, Marcus Vinícius Guimarães
Secundino, Nágila Francinete Costa
Monteiro, Wuelton Marcelo
Pimenta, Paulo Filemon Paolucci
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.other.none.fl_str_mv Quitinase
hematocrito
Malária
Matriz peritrófica
Plasmodium vivax
Trypsin
topic Quitinase
hematocrito
Malária
Matriz peritrófica
Plasmodium vivax
Trypsin
Chitinase
Hematocrit
Malaria
Peritrophic matrix
Plasmodium vivax
Trypsin
dc.subject.en.none.fl_str_mv Chitinase
Hematocrit
Malaria
Peritrophic matrix
Plasmodium vivax
Trypsin
description Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-06-26T17:35:35Z
dc.date.available.fl_str_mv 2019-06-26T17:35:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Djane Clarys Baia da et al. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis. Parasites and Vectors, v. 11, n. 1, p. 1-10, 2018.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/33677
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-018-2752-5
identifier_str_mv SILVA, Djane Clarys Baia da et al. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis. Parasites and Vectors, v. 11, n. 1, p. 1-10, 2018.
1756-3305
10.1186/s13071-018-2752-5
url https://arca.fiocruz.br/handle/icict/33677
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2019-06-26T17:35:35Z2019-06-26T17:35:35Z2018SILVA, Djane Clarys Baia da et al. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis. Parasites and Vectors, v. 11, n. 1, p. 1-10, 2018.1756-3305https://arca.fiocruz.br/handle/icict/3367710.1186/s13071-018-2752-5engBioMed CentralQuitinasehematocritoMaláriaMatriz peritróficaPlasmodium vivaxTrypsinChitinaseHematocritMalariaPeritrophic matrixPlasmodium vivaxTrypsinThe role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Grupo de Investigación QUIBIO, Departamento de Biología, Universidad Santiago de Cali, Valle del Cauca, Colômbia.Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM Brasil / Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.BACKGROUND: Plasmodium vivax is predominant in the Amazon region, and enhanced knowledge of its development inside a natural vector, Anopheles aquasalis, is critical for future strategies aimed at blocking parasite development. The peritrophic matrix (PM), a chitinous layer produced by the mosquito midgut in response to blood ingestion, is a protective barrier against pathogens. Plasmodium can only complete its life-cycle, and consequently be transmitted to a new host, after successfully passing this barrier. Interestingly, fully engorged mosquitoes that had a complete blood meal form a thicker, well-developed PM than ones that feed in small amounts. The amount of red blood cells (RBC) in the blood meal directly influences the production of digestive enzymes and can protect parasites from being killed during the meal digestion. A specific study interrupting the development of the PM associated with the proteolytic activity inhibition, and distinct RBC concentrations, during the P. vivax infection of the New World malaria vector An. aquasalis is expected to clarify whether these factors affect the parasite development. RESULTS: Absence of PM in the vector caused a significant reduction in P. vivax infection. However, the association of chitinase with trypsin inhibitor restored infection rates to those of mosquitoes with a structured PM. Also, only the ingestion of trypsin inhibitor by non-chitinase treated mosquitoes increased the infection intensity. Moreover, the RBC concentration in the infected P. vivax blood meal directly influenced the infection rate and its intensity. A straight correlation was observed between RBC concentrations and infection intensity. CONCLUSIONS: This study established that there is a balance between the PM role, RBC concentration and digestive enzyme activity influencing the establishment and development of P. vivax infection inside An. aquasalis. Our results indicate that the absence of PM in the midgut facilitates digestive enzyme dispersion throughout the blood meal, causing direct damage to P. vivax. On the other hand, high RBC concentrations support a better and thick, well-developed PM and protect P. vivax from being killed. Further studies of this complex system may provide insights into other details of the malaria vector response to P. vivax infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZSilva, Djane Clarys Baia daAlvarez, Luis Carlos SalazarLizcano, Omaira VeraCosta, Fabio Trindade MaranhãoLopes, Stefanie Costa PintoOrfanó, Alessandra SilvaPascoal, Denner OliveiraPimenta, Rafael NacifRodriguez, Iria CabralGuerra, Maria das Graças Vale BarbosaLacerda, Marcus Vinícius GuimarãesSecundino, Nágila Francinete CostaMonteiro, Wuelton MarceloPimenta, Paulo Filemon PaolucciLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://arca.fiocruz.br/bitstreams/45c542db-0628-4c17-8e89-68a77f2a246e/download9193a7c197bc67acd023525e72a03240MD51falseAnonymousREADORIGINALThe role of the peritrophic matrix .pdfThe role of the peritrophic matrix .pdfapplication/pdf10772262https://arca.fiocruz.br/bitstreams/370b5cb6-2ed3-49d7-835c-20fc4641d01e/downloadd36cfaf152937c4735da0d1eb9e0ec02MD52trueAnonymousREADTEXTThe role of the peritrophic matrix .pdf.txtThe role of the peritrophic matrix .pdf.txtExtracted texttext/plain734https://arca.fiocruz.br/bitstreams/cf0779f7-67cc-4725-9397-2b53cd5c16ae/download9f94d6b957621f59eb959160a0fafc33MD57falseAnonymousREADTHUMBNAILThe role of the peritrophic matrix .pdf.jpgThe role of the peritrophic matrix .pdf.jpgGenerated Thumbnailimage/jpeg36529https://arca.fiocruz.br/bitstreams/8e98b86d-8c9d-4431-87b0-ad680f8d8962/download548f8279c8020486cc720c90664a4007MD58falseAnonymousREADicict/336772025-12-10 14:00:49.934open.accessoai:arca.fiocruz.br:icict/33677https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T17:00:49Repositório Institucional da FIOCRUZ (ARCA) - 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