Induced reproduction of dourado (Salminus brasiliensis): fertilization with sperm cryopreserved in ACP®-104
Experiments were conducted to improve the fertilization rate of cryopreserved semen of dourado, Salminus brasiliensis. The experiment tested two cryoprotectant solutions at different semen: cryoprotectant ratios (1:5, 1:15, 1:25 and 1:50). The standard solution for the species (mixture of dimethyl s...
| Autores: | , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | Brasil |
| Recursos: | Universidade Estadual de Maringá (UEM) |
| Repositorio: | Acta Scientiarum. Animal Sciences (Online) |
| Idioma: | inglés |
| OAI Identifier: | oai:periodicos.uem.br/ojs:article/28838 |
| Acesso em linha: | https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/28838 |
| Access Level: | acceso abierto |
| Palavra-chave: | cryopreservation sperm fertilization cryoprotectant coconut water |
| Resumo: | Experiments were conducted to improve the fertilization rate of cryopreserved semen of dourado, Salminus brasiliensis. The experiment tested two cryoprotectant solutions at different semen: cryoprotectant ratios (1:5, 1:15, 1:25 and 1:50). The standard solution for the species (mixture of dimethyl sulfoxide, glucose, egg yolk and distilled water) was compared to a 350 mOsm ACP®-104 solution, which is composed of powdered coconut water diluted in distilled water and methylglycol. Differences between the dilutions tested were significant only for ACP®. The fertilization potential by using the standard solution at the lowest dilution (1:5) is equated when the sperm is diluted in ACP® at 1:25 or 1:50. These results show that the standard solution is the most suitable for the cryopreservation of dourado sperm, since the dilution did not alter the fertilization rate, requiring smaller storage space. |
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