The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate

Permanent bilateral occlusion of the common carotid arteries (2VO) in the rat has been established as a valid experimental model to investigate the effects of chronic cerebral hypoperfusion on cognitive function and neurodegenerative processes. Our aim was to compare the cognitive and morphological...

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Autores: Cechetti, Fernanda, Worm, Paulo Valdeci, Silva, Lenir Orlandi Pereira, Siqueira, Ionara Rodrigues, Netto, Carlos Alexandre
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/30588
Acceso en línea:http://hdl.handle.net/10183/30588
Access Level:acceso abierto
Palabra clave:Memória
Hipocampo
Hipoperfusão crônica
Chronic cerebral hypoperfusion
Spatial memory
Hippocampus
Striatum
Water maze
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spelling 2011-08-04T06:01:26Z20100100-879Xhttp://hdl.handle.net/10183/30588000773158Permanent bilateral occlusion of the common carotid arteries (2VO) in the rat has been established as a valid experimental model to investigate the effects of chronic cerebral hypoperfusion on cognitive function and neurodegenerative processes. Our aim was to compare the cognitive and morphological outcomes following the standard 2VO procedure, in which there is concomitant artery ligation, with those of a modified protocol, with a 1-week interval between artery occlusions to avoid an abrupt reduction of cerebral blood flow, as assessed by animal performance in the water maze and damage extension to the hippocampus and striatum. Male Wistar rats (N = 47) aged 3 months were subjected to chronic hypoperfusion by permanent bilateral ligation of the common carotid arteries using either the standard or the modified protocol, with the right carotid being the first to be occluded. Three months after the surgical procedure, rat performance in the water maze was assessed to investigate long-term effects on spatial learning and memory and their brains were processed in order to estimate hippocampal volume and striatal area. Both groups of hypoperfused rats showed deficits in reference (F(8,172) = 7.0951, P < 0.00001) and working spatial memory [2nd (F(2,44) = 7.6884, P < 0.001), 3rd (F(2,44) = 21.481, P < 0.00001) and 4th trials (F(2,44) = 28.620, P < 0.0001)]; however, no evidence of tissue atrophy was found in the brain structures studied. Despite similar behavioral and morphological outcomes, the rats submitted to the modified protocol showed a significant increase in survival rate, during the 3 months of the experiment (P < 0.02).application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 43, no. 12 (Dez. 2010), p. 1178-1183MemóriaHipocampoHipoperfusão crônicaChronic cerebral hypoperfusionSpatial memoryHippocampusStriatumWater mazeThe modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rateinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSCechetti, FernandaWorm, Paulo ValdeciSilva, Lenir Orlandi PereiraSiqueira, Ionara RodriguesNetto, Carlos AlexandreTEXT000773158.pdf.txt000773158.pdf.txtExtracted Texttext/plain28509http://www.lume.ufrgs.br/bitstream/10183/30588/2/000773158.pdf.txtc47f5b922780f9ad939f13cf9e57ce02MD52ORIGINAL000773158.pdf000773158.pdfTexto completo (inglês)application/pdf870171http://www.lume.ufrgs.br/bitstream/10183/30588/1/000773158.pdfc98b8f4ef3367b5274241bad383d5f27MD51THUMBNAIL000773158.pdf.jpg000773158.pdf.jpgGenerated Thumbnailimage/jpeg2046http://www.lume.ufrgs.br/bitstream/10183/30588/3/000773158.pdf.jpg69388d0e782cfb86d32c49e8b27dfbacMD5310183/305882021-11-20 05:50:40.879573oai:www.lume.ufrgs.br:10183/30588Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2021-11-20T07:50:40Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
title The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
spellingShingle The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
Cechetti, Fernanda
Memória
Hipocampo
Hipoperfusão crônica
Chronic cerebral hypoperfusion
Spatial memory
Hippocampus
Striatum
Water maze
title_short The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
title_full The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
title_fullStr The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
title_full_unstemmed The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
title_sort The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
dc.creator.none.fl_str_mv Cechetti, Fernanda
Worm, Paulo Valdeci
Silva, Lenir Orlandi Pereira
Siqueira, Ionara Rodrigues
Netto, Carlos Alexandre
author Cechetti, Fernanda
author_facet Cechetti, Fernanda
Worm, Paulo Valdeci
Silva, Lenir Orlandi Pereira
Siqueira, Ionara Rodrigues
Netto, Carlos Alexandre
author_role author
author2 Worm, Paulo Valdeci
Silva, Lenir Orlandi Pereira
Siqueira, Ionara Rodrigues
Netto, Carlos Alexandre
author2_role author
author
author
author
dc.subject.por.fl_str_mv Memória
Hipocampo
Hipoperfusão crônica
topic Memória
Hipocampo
Hipoperfusão crônica
Chronic cerebral hypoperfusion
Spatial memory
Hippocampus
Striatum
Water maze
dc.subject.eng.fl_str_mv Chronic cerebral hypoperfusion
Spatial memory
Hippocampus
Striatum
Water maze
description Permanent bilateral occlusion of the common carotid arteries (2VO) in the rat has been established as a valid experimental model to investigate the effects of chronic cerebral hypoperfusion on cognitive function and neurodegenerative processes. Our aim was to compare the cognitive and morphological outcomes following the standard 2VO procedure, in which there is concomitant artery ligation, with those of a modified protocol, with a 1-week interval between artery occlusions to avoid an abrupt reduction of cerebral blood flow, as assessed by animal performance in the water maze and damage extension to the hippocampus and striatum. Male Wistar rats (N = 47) aged 3 months were subjected to chronic hypoperfusion by permanent bilateral ligation of the common carotid arteries using either the standard or the modified protocol, with the right carotid being the first to be occluded. Three months after the surgical procedure, rat performance in the water maze was assessed to investigate long-term effects on spatial learning and memory and their brains were processed in order to estimate hippocampal volume and striatal area. Both groups of hypoperfused rats showed deficits in reference (F(8,172) = 7.0951, P < 0.00001) and working spatial memory [2nd (F(2,44) = 7.6884, P < 0.001), 3rd (F(2,44) = 21.481, P < 0.00001) and 4th trials (F(2,44) = 28.620, P < 0.0001)]; however, no evidence of tissue atrophy was found in the brain structures studied. Despite similar behavioral and morphological outcomes, the rats submitted to the modified protocol showed a significant increase in survival rate, during the 3 months of the experiment (P < 0.02).
publishDate 2010
dc.date.issued.fl_str_mv 2010
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 43, no. 12 (Dez. 2010), p. 1178-1183
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instname:Universidade Federal do Rio Grande do Sul (UFRGS)
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reponame_str Repositório Institucional da UFRGS
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