A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes

Foguel, Marcos Vinicius [UNESP]; Giordano, Gabriela Furlan [UNESP]; Sylos, Celia Maria de [UNESP]; Carlos, Iracilda Zeppone [UNESP]; Pupim Ferreira, Antonio Aparecido [UNESP]; Benedetti, Assis Vicente [UNESP]; Yamanaka, Hideko [UNESP]

A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes

This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B-1 (AFB(1)). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The...

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Detalles Bibliográficos
Autores:	Foguel, Marcos Vinicius [UNESP], Giordano, Gabriela Furlan [UNESP], Sylos, Celia Maria de [UNESP], Carlos, Iracilda Zeppone [UNESP], Pupim Ferreira, Antonio Aparecido [UNESP], Benedetti, Assis Vicente [UNESP], Yamanaka, Hideko [UNESP]
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2016
País:	Brasil
Institución:	Universidade Estadual Paulista (UNESP)
Repositorio:	Repositório Institucional da UNESP
Idioma:	inglés
OAI Identifier:	oai:repositorio.unesp.br:11449/161873
Acceso en línea:	http://dx.doi.org/10.3390/chemosensors4030017 http://hdl.handle.net/11449/161873
Access Level:	acceso abierto
Palabra clave:	aflatoxin B-1 immunosensor electrochemical impedance spectroscopy

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spelling	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodesaflatoxin B-1immunosensorelectrochemical impedance spectroscopyThis work describes the investigation of a label-free immunosensor for the detection of aflatoxin B-1 (AFB(1)). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB(1). The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB(1) antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody-antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 x 10(-9) to 1 x 10(-7) mol . L-1 (1.56-31.2 ng . mL(-1)), limit of detection and limit of quantification, respectively, 3.6 x 10(-10) and 1.1 x 10(-9) mol . L-1 (0.11 and 0.34 ng.mL(-1)). The proposed immunosensor was applied to analyze peanut samples.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FP7-PEOPLE-IRSESConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP Univ Estadual Paulista, Inst Chem, Dept Analyt Chem, Rua Francisco Degni 55, BR-14800060 Araraquara, SP, BrazilUNESP Univ Estadual Paulista, Dept Food & Nutr, Sch Pharmaceut Sci, Rodovia Araraquara Jau,Km 01 S-N,Campos Ville, BR-14800903 Araraquara, SP, BrazilUNESP Univ Estadual Paulista, Dept Clin Anal, Sch Pharmaceut Sci, Rodovia Araraquara Jau,Km 01 S-N,Campos Ville, BR-14800903 Araraquara, SP, BrazilUNESP Univ Estadual Paulista, Inst Chem, Dept Analyt Chem, Rua Francisco Degni 55, BR-14800060 Araraquara, SP, BrazilUNESP Univ Estadual Paulista, Dept Food & Nutr, Sch Pharmaceut Sci, Rodovia Araraquara Jau,Km 01 S-N,Campos Ville, BR-14800903 Araraquara, SP, BrazilUNESP Univ Estadual Paulista, Dept Clin Anal, Sch Pharmaceut Sci, Rodovia Araraquara Jau,Km 01 S-N,Campos Ville, BR-14800903 Araraquara, SP, BrazilFAPESP: 2008/07729-8FP7-PEOPLE-IRSES: 2008-230849CNPq: 309275/2012-1Mdpi AgUniversidade Estadual Paulista (Unesp)2018-11-26T17:04:29Z2018-11-26T17:04:29Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.3390/chemosensors4030017Chemosensors. Basel: Mdpi Ag, v. 4, n. 3, 10 p., 2016.2227-9040http://hdl.handle.net/11449/16187310.3390/chemosensors4030017WOS:000382480000007WOS000382480000007.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosensorsinfo:eu-repo/semantics/openAccessFoguel, Marcos Vinicius [UNESP]Giordano, Gabriela Furlan [UNESP]Sylos, Celia Maria de [UNESP]Carlos, Iracilda Zeppone [UNESP]Pupim Ferreira, Antonio Aparecido [UNESP]Benedetti, Assis Vicente [UNESP]Yamanaka, Hideko [UNESP]2025-05-28T07:53:08Zoai:repositorio.unesp.br:11449/161873Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-05-28T07:53:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes
title	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes
spellingShingle	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes Foguel, Marcos Vinicius [UNESP] aflatoxin B-1 immunosensor electrochemical impedance spectroscopy
title_short	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes
title_full	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes
title_fullStr	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes
title_full_unstemmed	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes
title_sort	A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes
dc.creator.none.fl_str_mv	Foguel, Marcos Vinicius [UNESP] Giordano, Gabriela Furlan [UNESP] Sylos, Celia Maria de [UNESP] Carlos, Iracilda Zeppone [UNESP] Pupim Ferreira, Antonio Aparecido [UNESP] Benedetti, Assis Vicente [UNESP] Yamanaka, Hideko [UNESP]
author	Foguel, Marcos Vinicius [UNESP]
author_facet	Foguel, Marcos Vinicius [UNESP] Giordano, Gabriela Furlan [UNESP] Sylos, Celia Maria de [UNESP] Carlos, Iracilda Zeppone [UNESP] Pupim Ferreira, Antonio Aparecido [UNESP] Benedetti, Assis Vicente [UNESP] Yamanaka, Hideko [UNESP]
author_role	author
author2	Giordano, Gabriela Furlan [UNESP] Sylos, Celia Maria de [UNESP] Carlos, Iracilda Zeppone [UNESP] Pupim Ferreira, Antonio Aparecido [UNESP] Benedetti, Assis Vicente [UNESP] Yamanaka, Hideko [UNESP]
author2_role	author author author author author author
dc.contributor.none.fl_str_mv	Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv	aflatoxin B-1 immunosensor electrochemical impedance spectroscopy
topic	aflatoxin B-1 immunosensor electrochemical impedance spectroscopy
description	This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B-1 (AFB(1)). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB(1). The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB(1) antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody-antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 x 10(-9) to 1 x 10(-7) mol . L-1 (1.56-31.2 ng . mL(-1)), limit of detection and limit of quantification, respectively, 3.6 x 10(-10) and 1.1 x 10(-9) mol . L-1 (0.11 and 0.34 ng.mL(-1)). The proposed immunosensor was applied to analyze peanut samples.
publishDate	2016
dc.date.none.fl_str_mv	2016-09-01 2018-11-26T17:04:29Z 2018-11-26T17:04:29Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	http://dx.doi.org/10.3390/chemosensors4030017 Chemosensors. Basel: Mdpi Ag, v. 4, n. 3, 10 p., 2016. 2227-9040 http://hdl.handle.net/11449/161873 10.3390/chemosensors4030017 WOS:000382480000007 WOS000382480000007.pdf
url	http://dx.doi.org/10.3390/chemosensors4030017 http://hdl.handle.net/11449/161873
identifier_str_mv	Chemosensors. Basel: Mdpi Ag, v. 4, n. 3, 10 p., 2016. 2227-9040 10.3390/chemosensors4030017 WOS:000382480000007 WOS000382480000007.pdf
dc.language.iso.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	Chemosensors
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	10 application/pdf
dc.publisher.none.fl_str_mv	Mdpi Ag
publisher.none.fl_str_mv	Mdpi Ag
dc.source.none.fl_str_mv	Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP
instname_str	Universidade Estadual Paulista (UNESP)
instacron_str	UNESP
institution	UNESP
reponame_str	Repositório Institucional da UNESP
collection	Repositório Institucional da UNESP
repository.name.fl_str_mv	Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv	repositoriounesp@unesp.br
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score	15.301603

A Low-Cost Label-Free AFB(1) Impedimetric Immunosensor Based on Functionalized CD-Trodes

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