Maximal lactate steady state for aerobic evaluation of swimming mice

The maximal lactate steady state (MLSS) has been considered the gold standard method to determine aerobic/anaerobic metabolism transition during continuous exercise executed by human beings and rats. Therefore, the aim of the present study was to determine MLSS for aerobic evaluation in swimming mic...

ver descrição completa

Detalhes bibliográficos
Autores: Gobatto, Claudio Alexandre [UNESP], de Barros Manchado-Gobatto, Fúlvia [UNESP], Carneiro, Ligia Giuzio [UNESP], de Araujo, Gustavo Gomes [UNESP], Masselli Dos Reis, Ivan Gustavo [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/228285
Acesso em linha:http://dx.doi.org/10.1017/S1755254009990109
http://hdl.handle.net/11449/228285
Access Level:acceso abierto
Palavra-chave:aerobic exercise intensity
anaerobic threshold
blood lactate
swimming mice
Descrição
Resumo:The maximal lactate steady state (MLSS) has been considered the gold standard method to determine aerobic/anaerobic metabolism transition during continuous exercise executed by human beings and rats. Therefore, the aim of the present study was to determine MLSS for aerobic evaluation in swimming mice. Twenty-five adult male mice (90-day-old animals) were adapted to the deep aquatic environment at the temperature of 31 ± 1°C. The mice were submitted to five continuous exercise loads of 3, 4, 5, 6 and 7% of the body weight (bw) tied to the back, executed with 25-min duration and 48-h intervals between them. Blood samples were collected from the tail during swimming exercise (rest, 5, 10, 15, 20 and 25 min) for blood lactate concentration (LAC) determinations. The individual MLSS was considered as the highest intensity in which the increase on the LAC was equal to or below 1 mmol l− 1 from the 10th to the 25th minute of exercise. The results showed that 36% of the swimming mice presented MLSS at 4% bw, 20% at 3% bw and 6% bw, 16% at 5% bw and 8% at 7% bw. The LAC at the MLSS was 5.78 ± 0.29 mmol l− 1 (4.40–6.67 mmol l− 1). These results indicate that the MLSS of mice swimming with additional weight for the final 15 of 25 min of exercise could be determined. © 2009, Cambridge University Press. All rights reserved.