Simultaneous determination of paracetamol, dextromethorphan, chlorpheniramine, pseudoephedrine and major impurities of paracetamol and pseudoephedrine by using capillary electrophoresis
A capillary electrophoresis method was developed for the first time and optimized for thedetermination of paracetamol, pseudoephedrine, dextromethorphan, chlorpheniramine,4-aminophenol and ephedrine in tablet formulation. Optimum electrophoretic conditions wereachieved by using a background electrol...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Brasil |
| Institución: | Universidade de São Paulo (USP) |
| Repositorio: | Brazilian Journal of Pharmaceutical Sciences |
| Idioma: | inglés |
| OAI Identifier: | oai:revistas.usp.br:article/204237 |
| Acceso en línea: | https://www.revistas.usp.br/bjps/article/view/204237 |
| Access Level: | acceso abierto |
| Palabra clave: | Capillary electrophoresis Chlorpheniramine Dextromethorphan Paracetamol Pseudoephedrine |
| Sumario: | A capillary electrophoresis method was developed for the first time and optimized for thedetermination of paracetamol, pseudoephedrine, dextromethorphan, chlorpheniramine,4-aminophenol and ephedrine in tablet formulation. Optimum electrophoretic conditions wereachieved by using a background electrolyte of 75 mmol L-1 sodium borate buffer at pH 8.0, acapillary temperature of 30°C, a separation voltage of 30 kV and a pressure injection of the sampleat 50 mbar for 10 s. Calibration graphs showed a good linearity with a coefficient of determination(R2) of at least 0.999 for all compounds. Intraday and interday precision (expressed as relativestandard deviation (RSD) %) were lower than 1.39% for capillary electrophoresis method. Thedeveloped method was demonstrated to be simple and rapid for the determination of paracetamol,pseudoephedrine, dextromethorphan, chlorpheniramine, 4-aminophenol and ephedrine in tabletformulation providing recoveries in the range between 99.62 and 100.57% for all analytes. |
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