DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite

Some antioxidant compounds have a pro-oxidant effect in the presence of transition metal ions, due to the reduction of Mn+ to M(n-1)+ with simultaneous formation of free radicals, which then promote DNA damage. In the present study, we evaluated the pUC19 DNA damage in a solution containing Cu(II) a...

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Authors: Boscolo Requeijo, Thais, Pires Serrano, Silvia Helena, Dorigan Moya, Horacio
Format: article
Status:Published version
Publication Date:2022
Country:Brasil
Institution:Universidade de São Paulo (USP)
Repository:Brazilian Journal of Pharmaceutical Sciences
Language:English
OAI Identifier:oai:revistas.usp.br:article/204110
Online Access:https://www.revistas.usp.br/bjps/article/view/204110
Access Level:Open access
Keyword:Gel electrophoresis. DNA damage. copper. ascorbic acid. sulfite.
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spelling DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfiteGel electrophoresis. DNA damage. copper. ascorbic acid. sulfite.Some antioxidant compounds have a pro-oxidant effect in the presence of transition metal ions, due to the reduction of Mn+ to M(n-1)+ with simultaneous formation of free radicals, which then promote DNA damage. In the present study, we evaluated the pUC19 DNA damage in a solution containing Cu(II) and ascorbic acid (AA) or S(IV) saturated with air by agarose gel electrophoresis. Our results showed that this damage decreases if AA and S(IV) are simultaneously added. This study also illustrates the importance of Cu(II) in this process, as no DNA damage was observed when AA or S(IV) were present in the absence of this metallic ion. Our data showed that DNA preservation depends on the concentration of AA and S(IV) and occurs when the [S(IV)]:[AA] ratio ranges from 1:1 to 20:1. Absorbance measurements and thermodynamic data show that no reaction occurs between AA and S(IV) when this mixture (pH 5.5) is added to pUC-19 DNA. The presence of dissolved oxygen may be the cause of AA consumption in the mixture of these two antioxidants, which subsequently decreases DNA damage.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.revistas.usp.br/bjps/article/view/20411010.1590/s2175-979020200004181084Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)Brazilian Journal of Pharmaceutical Sciences; v. 57 (2021)Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/204110/187726Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBoscolo Requeijo, ThaisPires Serrano, Silvia HelenaDorigan Moya, Horacio2022-11-09T19:19:51Zoai:revistas.usp.br:article/204110Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2022-11-09T19:19:51Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
title DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
spellingShingle DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
Boscolo Requeijo, Thais
Gel electrophoresis. DNA damage. copper. ascorbic acid. sulfite.
title_short DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
title_full DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
title_fullStr DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
title_full_unstemmed DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
title_sort DNA damage in a solution containing copper(II) ions and ascorbic acid: Effect of the presence of sulfite
dc.creator.none.fl_str_mv Boscolo Requeijo, Thais
Pires Serrano, Silvia Helena
Dorigan Moya, Horacio
author Boscolo Requeijo, Thais
author_facet Boscolo Requeijo, Thais
Pires Serrano, Silvia Helena
Dorigan Moya, Horacio
author_role author
author2 Pires Serrano, Silvia Helena
Dorigan Moya, Horacio
author2_role author
author
dc.subject.por.fl_str_mv Gel electrophoresis. DNA damage. copper. ascorbic acid. sulfite.
topic Gel electrophoresis. DNA damage. copper. ascorbic acid. sulfite.
description Some antioxidant compounds have a pro-oxidant effect in the presence of transition metal ions, due to the reduction of Mn+ to M(n-1)+ with simultaneous formation of free radicals, which then promote DNA damage. In the present study, we evaluated the pUC19 DNA damage in a solution containing Cu(II) and ascorbic acid (AA) or S(IV) saturated with air by agarose gel electrophoresis. Our results showed that this damage decreases if AA and S(IV) are simultaneously added. This study also illustrates the importance of Cu(II) in this process, as no DNA damage was observed when AA or S(IV) were present in the absence of this metallic ion. Our data showed that DNA preservation depends on the concentration of AA and S(IV) and occurs when the [S(IV)]:[AA] ratio ranges from 1:1 to 20:1. Absorbance measurements and thermodynamic data show that no reaction occurs between AA and S(IV) when this mixture (pH 5.5) is added to pUC-19 DNA. The presence of dissolved oxygen may be the cause of AA consumption in the mixture of these two antioxidants, which subsequently decreases DNA damage.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/204110
10.1590/s2175-979020200004181084
url https://www.revistas.usp.br/bjps/article/view/204110
identifier_str_mv 10.1590/s2175-979020200004181084
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/204110/187726
dc.rights.driver.fl_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)
Brazilian Journal of Pharmaceutical Sciences; v. 57 (2021)
Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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